Anti-Chromodomain helicase DNA binding protein 9 抗体 (ab114115)
Key features and details
- Rabbit polyclonal to Chromodomain helicase DNA binding protein 9
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Chromodomain helicase DNA binding protein 9 antibody -
製品の詳細
Rabbit polyclonal to Chromodomain helicase DNA binding protein 9 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Chicken, Cow, Dog, Chimpanzee, Rhesus monkey, Orangutan, Elephant
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免疫原
Synthetic peptide corresponding to Human Chromodomain helicase DNA binding protein 9 aa 1-100.
Database link: Q3L8U1 -
ポジティブ･コントロール
- HeLa and 293T whole cell lysates.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7-8 -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
ab114115 was affinity purified using an epitope specific to Chromodomain helicase DNA binding protein 9 immobilized on solid support. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab114115の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000 - 1/5000. Predicted molecular weight: 326 kDa.
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| IP |
Use at 2-5 µg/mg of lysate.
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| 特記事項 |
|---|
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WB
1/1000 - 1/5000. Predicted molecular weight: 326 kDa. |
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IP
Use at 2-5 µg/mg of lysate. |
ターゲット情報
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細胞内局在
Cytoplasmic and Nuclear -
参照データベース
- Entrez Gene: 80205 Human
- Entrez Gene: 109151 Mouse
- Entrez Gene: 307726 Rat
- SwissProt: Q3L8U1 Human
- SwissProt: Q8BYH8 Mouse
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別名
- 1810014J18Rik antibody
- 9030205D12Rik antibody
- A330063D19Rik antibody
see all
画像
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Western blot - Anti-Chromodomain helicase DNA binding protein 9 antibody (ab114115)All lanes : Anti-Chromodomain helicase DNA binding protein 9 antibody (ab114115) at 0.4 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 326 kDa
Exposure time: 30 seconds -
Immunoprecipitation - Anti-Chromodomain helicase DNA binding protein 9 antibody (ab114115)Detection of Chromodomain helicase DNA binding protein 9 by Western Blot of Immunprecipitate.
Lane 1: ab114115 at 1µg/ml staining Chromodomain helicase DNA binding protein 9 in HeLa whole cell lysate immunoprecipitated using ab114115 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Lane 2: Control IgG
Detection: Chemiluminescence with exposure time of 30 seconds.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab114115 は 1 報の論文で使用されています。
- Chen H et al. MiR-22-3p Suppresses Vascular Remodeling and Oxidative Stress by Targeting CHD9 during the Development of Hypertension. J Vasc Res 58:180-190 (2021). PubMed: 33794525