Anti-DDIT3 (phospho S30) 抗体 (ab63392)
Key features and details
- Rabbit polyclonal to CHOP (phospho S30 + S30)
- Suitable for: ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-DDIT3 (phospho S30) antibody
DDIT3 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to DDIT3 (phospho S30) -
由来種
Rabbit -
特異性
This antibody detects endogenous levels of GADD153 only when phosphorylated at serine 30. -
アプリケーション
適用あり: ICC/IF, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse
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免疫原
Synthetic peptide corresponding to Human DDIT3 (phospho S30).
Database link: P35638 -
ポジティブ･コントロール
- A549 cells and human breast carcinoma tissue
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab63392の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
1/500 - 1/1000.
|
|
| IHC-P |
1/50 - 1/100.
|
| 特記事項 |
|---|
|
ICC/IF
1/500 - 1/1000. |
|
IHC-P
1/50 - 1/100. |
ターゲット情報
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機能
Inhibits the DNA-binding activity of C/EBP and LAP by forming heterodimers that cannot bind DNA. -
関連疾患
Note=A chromosomal aberration involving DDIT3 is found in a patient with malignant myxoid liposarcoma. Translocation t(12;16)(q13;p11) with FUS. -
配列類似性
Belongs to the bZIP family.
Contains 1 bZIP domain. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 1649 Human
- Entrez Gene: 13198 Mouse
- Omim: 126337 Human
- SwissProt: P35638 Human
- SwissProt: P35639 Mouse
- Unigene: 505777 Human
- Unigene: 728989 Human
- Unigene: 110220 Mouse
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別名
- C/EBP homologous protein antibody
- C/EBP Homology Protein antibody
- C/EBP zeta antibody
see all
画像
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Immunocytochemistry/ Immunofluorescence - Anti-DDIT3 (phospho S30) antibody (ab63392)Immunofluorescence analysis of A549 cells, using ab63392 at 1:500 dilution.
Left image untreated.Right image treated with phospho peptide. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDIT3 (phospho S30) antibody (ab63392)Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using ab63392 at 1:50 dilution.
Left image untreated.Right image treated with phospho peptide.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab63392 は 1 報の論文で使用されています。
- Tian H et al. D4F alleviates the C/EBP homologous protein-mediated apoptosis in glycated high-density lipoprotein-treated macrophages by facilitating autophagy. Exp Biol Med (Maywood) 246:2595-2609 (2021). PubMed: 34525858