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AB27441

Anti-Cholecystokinin 抗体

Anti-Cholecystokinin antibody

5

(1 Review)

|

(9 Publications)

Rabbit Polyclonal Cholecystokinin antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 9 publications.

別名を表示する

Cholecystokinin, CCK

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-Cholecystokinin antibody (AB27441)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Cholecystokinin antibody (AB27441)

ICC/IF image of ab27441 stained CaCO2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab27441 at 10μg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.

Western blot - Anti-Cholecystokinin antibody (AB27441)
  • WB

Unknown

Western blot - Anti-Cholecystokinin antibody (AB27441)

All lanes:

Western blot - Anti-Cholecystokinin antibody (ab27441) at 1/25 dilution

All lanes:

Brain cell lysate

Predicted band size: 13 kDa

Observed band size: 12 kDa

false

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

No

交差種

Human

アプリケーション

IHC-P, ICC/IF, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p>Incubate for 10 minutes at room temperature.</p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Immunogen
バッファー組成
pH: 7.6 Preservative: 0.1% Sodium azide Constituents: PBS, 1% BSA
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Cholecystokinin often referenced as CCK is a peptide hormone with a mass of approximately 8 kDa. It plays a mechanical role by being released from the I-cells in the small intestine particularly the duodenum. This release occurs in response to nutrients in the gut such as fats and proteins. Cholecystokinin acts by binding to its receptors CCK-A and CCK-B to execute its functions. In addition to the gut CCK is expressed in the central nervous system where it contributes to diverse neurological functions.
Biological function summary

Beyond its presence in the digestive system cholecystokinin is important for stimulating digestive enzymes secretion from the pancreas and bile release from the gallbladder. This peptide is also part of the central nervous system where it influences appetite control satiety and anxiety regulation. While not part of a classic protein complex CCK interacts with various receptors and enzymes to perform its roles in these physiological processes.

Pathways

Several important physiological pathways include cholecystokinin to regulate digestive processes and satiety signals. It is significant in the digestion pathway interacting chiefly with proteins such as trypsinogen and pancreatic lipase enhancing the breakdown of nutrients. In the neural pathway CCK influences neurotransmitter release including dopamine and serotonin highlighting its role in appetite suppression and mood regulation.

Cholecystokinin has been implicated in gastrointestinal and neurological conditions. It links to gallstone disease by influencing bile composition and flow which can affect the development of gallstones. Additionally CCK has connections to anxiety disorders given its impact on neurotransmitter systems within the brain. The peptide's engagement with CCK receptors makes it a target for studying these disorders as alterations in its signaling can contribute to disease pathology.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

This peptide hormone induces gall bladder contraction and the release of pancreatic enzymes in the gut. Its function in the brain is not clear. Binding to CCK-A receptors stimulates amylase release from the pancreas, binding to CCK-B receptors stimulates gastric acid secretion.
See full target information Cholecystokinin
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文献 (9)

Recent publications for all applications. Explore the full list and refine your search

eLife 12: PubMed36810133

2023

Enteroendocrine cell lineages that differentially control feeding and gut motility.

Applications

Unspecified application

Species

Unspecified reactive species

Marito Hayashi,Judith A Kaye,Ella R Douglas,Narendra R Joshi,Fiona M Gribble,Frank Reimann,Stephen D Liberles

Frontiers in oncology 12:1098728 PubMed36733361

2023

CCKAR is a biomarker for prognosis and asynchronous brain metastasis of non-small cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Nan Liang,Suohui Sun,Zheng Li,Tao Wu,Chunpu Zhang,Tao Xin

Poultry science 102:102273 PubMed36436379

2022

Cholecystokinin (CCK) and its receptors (CCK1R and CCK2R) in chickens: functional analysis and tissue expression.

Applications

Unspecified application

Species

Unspecified reactive species

Yiping Wan,Qiuyang Deng,Zhichun Zhou,Yue Deng,Jiannan Zhang,Juan Li,Yajun Wang

JID innovations : skin science from molecules to population health 2:100153 PubMed36262666

2022

Cholecystokinin Receptor Antagonist Suppresses Melanoma Growth by Inducing Apoptosis of Tumor Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Atsuko Funakoshi,Tetsuya Honda,Taisuke Ito,Yoshiki Tokura

Scientific reports 11:4933 PubMed33654150

2021

First evidence for the presence of amino acid sensing mechanisms in the fish gastrointestinal tract.

Applications

Unspecified application

Species

Unspecified reactive species

Jessica Calo,Ayelén M Blanco,Sara Comesaña,Marta Conde-Sieira,Sofia Morais,José L Soengas

The Journal of dermatology 47:1454-1456 PubMed32794215

2020

Secukinumab promotes engagement of cholecystokinin and its receptor in epidermal keratinocytes of psoriasis patients.

Applications

Unspecified application

Species

Unspecified reactive species

Atsuko Funakoshi,Toshiharu Fujiyama,Kazuki Tatsuno,Takatoshi Shimauchi,Yoshiki Tokura

Anais da Academia Brasileira de Ciencias 92:e20181165 PubMed32556047

2020

Localization and distribution of CCK-8, NPY, Leu-ENK-, and Ghrelin- in the digestive tract of Prochilodus lineatus (Valenciennes, 1836).

Applications

Unspecified application

Species

Unspecified reactive species

Carlos E Barrios,Juan JosÉ SantinÓn,Hugo A Domitrovic,SebastiÁn SÁnchez,David R HernÁndez

Journal of immunology (Baltimore, Md. : 1950) 202:2609-2615 PubMed30902899

2019

Cholecystokinin Downregulates Psoriatic Inflammation by Its Possible Self-Regulatory Effect on Epidermal Keratinocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Atsuko Funakoshi,Kazuki Tatsuno,Takatoshi Shimauchi,Toshiharu Fujiyama,Taisuke Ito,Yoshiki Tokura

Gastroenterology 137:2052-62 PubMed19737569

2009

Foxa1 and Foxa2 control the differentiation of goblet and enteroendocrine L- and D-cells in mice.

Applications

IHC-P

Species

Mouse

Diana Z Ye,Klaus H Kaestner
View all publications
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