Anti-Chloramphenicol 抗体 (ab35658)
Key features and details
- Sheep polyclonal to Chloramphenicol
- Suitable for: ELISA, FPIA
- Reacts with: Species independent
- Isotype: IgG
製品の概要
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製品名
Anti-Chloramphenicol antibody
Chloramphenicol 一次抗体 製品一覧 -
製品の詳細
Sheep polyclonal to Chloramphenicol -
由来種
Sheep -
アプリケーション
適用あり: ELISA, FPIAmore details -
種交差性
交差種: Species independent -
免疫原
Aromatic amino derivative of chloramphenicol (prepared by reduction of the nitro group by high-pressure hydrogenation) conjugated to KLH using the diazotisation procedure.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.20
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度
Protein G purified -
特記事項（精製）
Prepared by Ammonium Sulphate fractionation. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab35658の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ELISA | ||
| FPIA |
| 特記事項 |
|---|
ab35658 was used in a simple indirect ELISA with 400 ng of Chloramphenicol-BSA per well. The titres detailed above were elucidated using an HRP labelled secondary antibody/TMB detection system.
FPIA: 1/1500.
When tested by polarisation fluoroimmunoassay, the final titre dilution was found to be 0.5 ml tracer + 0.5 ml diluted antibody. Tracer is aromatic amino derivative of chloramphenicol labelled by reaction with fluorescein isothiocyanate (10 nmol/L).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
ターゲット情報
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関連性
Chloramphenicol is an antibiotic that was derived from the bacterium Streptomyces venezuelae. It was the first antibiotic to be manufactured synthetically on a large scale. Chloramphenicol is effective against a wide variety of microorganisms, but due to serious side effects (eg damage to the bone marrow) in humans, it is usually reserved for the treatment of serious and life threatening infections (eg typhoid fever). It is also used in eye drops or ointment to treat bacterial conjunctivitis.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab35658 は論文での使用が確認できていません。