Anti-Chk2 (phospho T68) 抗体 (ab85743)
Key features and details
- Rabbit polyclonal to Chk2 (phospho T68)
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Chk2 (phospho T68) antibody
Chk2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Chk2 (phospho T68) -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, IHC-P, WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Cow, Pig
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- This antibody produced a positive signal in Irradiated HeLa. It also gave a positive result in FFPE human colon adenocarcinoma tissue sections.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, 98.98% PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab85743の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
Use a concentration of 5 µg/ml.
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| IHC-P | (1) |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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| WB | (1) |
Use a concentration of 1 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 61 kDa).
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| 特記事項 |
|---|
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ICC/IF
Use a concentration of 5 µg/ml. |
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IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 61 kDa). |
ターゲット情報
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機能
Regulates cell cycle checkpoints and apoptosis in response to DNA damage, particularly to DNA double-strand breaks. Inhibits CDC25C phosphatase by phosphorylation on 'Ser-216', preventing the entry into mitosis. May also play a role in meiosis. Regulates the TP53 tumor suppressor through phosphorylation at 'Thr-18' and 'Ser-20'. -
組織特異性
High expression is found in testis, spleen, colon and peripheral blood leukocytes. Low expression is found in other tissues. -
関連疾患
Defects in CHEK2 are associated with Li-Fraumeni syndrome 2 (LFS2) [MIM:609265]; a highly penetrant familial cancer phenotype usually associated with inherited mutations in p53/TP53.
Defects in CHEK2 may be a cause of susceptibility to prostate cancer (PC) [MIM:176807]. It is a malignancy originating in tissues of the prostate. Most prostate cancers are adenocarcinomas that develop in the acini of the prostatic ducts. Other rare histopathologic types of prostate cancer that occur in approximately 5% of patients include small cell carcinoma, mucinous carcinoma, prostatic ductal carcinoma, transitional cell carcinoma, squamous cell carcinoma, basal cell carcinoma, adenoid cystic carcinoma (basaloid), signet-ring cell carcinoma and neuroendocrine carcinoma.
Defects in CHEK2 are found in some patients with osteogenic sarcoma (OSRC) [MIM:259500]. -
配列類似性
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CHK2 subfamily.
Contains 1 FHA domain.
Contains 1 protein kinase domain. -
翻訳後修飾
Phosphorylated by PLK4. -
細胞内局在
Nucleus; Nucleus. Isoform 10 is present throughout the cell and Nucleus > PML body. Nucleus > nucleoplasm. Recruited into PML bodies together with TP53. - Information by UniProt
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参照データベース
- Entrez Gene: 518897 Cow
- Entrez Gene: 11200 Human
- Entrez Gene: 50883 Mouse
- Entrez Gene: 100192447 Pig
- Entrez Gene: 114212 Rat
- Omim: 604373 Human
- SwissProt: O96017 Human
- SwissProt: Q9Z265 Mouse
see all -
別名
- CDS 1 antibody
- Cds1 antibody
- Cds1 homolog antibody
see all
画像
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Western blot - Anti-Chk2 (phospho T68) antibody (ab85743)All lanes : Anti-Chk2 (phospho T68) antibody (ab85743) at 1 µg/ml
Lane 1 : HeLa Whole Cell Lysate - Irradiated (5Gy)
Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 3 : HeLa Whole Cell Lysate - Irradiated (5Gy) with Human Chk2 (phospho T68) peptide (ab111650) at 1 µg/ml
Lane 4 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate with Human Chk2 (phospho T68) peptide (ab111650) at 1 µg/ml
Lane 5 : HeLa Whole Cell Lysate - Irradiated (5Gy) with Human Chk2 peptide (ab118179) at 1 µg/ml
Lane 6 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate with Human Chk2 peptide (ab118179) at 1 µg/ml
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 61 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?
Additional bands at: 145 kDa, 38 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutesSecondary antibody - anti-rabbit HRP (IgG H&L; ab97080)
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Immunocytochemistry/ Immunofluorescence - Anti-Chk2 (phospho T68) antibody (ab85743)ICC/IF image of ab85743 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab85743 at 5µg/ml overnight at +4°C. The secondary antibody (green) was goat anti rabbit DyLight® 488 (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in formaldehyde fixed (4%, 10min) HeLa cells at 5ug/ml.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chk2 (phospho T68) antibody (ab85743)IHC image of Chk2 staining in human colon adenocarcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab85743, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (10)
ab85743 は 10 報の論文で使用されています。
- Wu J et al. DNA Double-strand Break Signaling Is a Therapeutic Target in Head and Neck Cancer. Anticancer Res 41:5393-5403 (2021). PubMed: 34732408
- Haeger A et al. Collective cancer invasion forms an integrin-dependent radioresistant niche. J Exp Med 217:N/A (2020). PubMed: 31658985
- Xie C et al. Inhibition of autophagy aggravates DNA damage response and gastric tumorigenesis via Rad51 ubiquitination in response to H. pylori infection. Gut Microbes 11:1567-1589 (2020). PubMed: 32588736
- Mehta RK et al. Low-Dose Hsp90 Inhibitor Selectively Radiosensitizes HNSCC and Pancreatic Xenografts. Clin Cancer Res 26:5246-5257 (2020). PubMed: 32718999
- Wu J & Crowe DL Molecular and cellular basis of mammary gland fibrosis and cancer risk. Int J Cancer 144:2239-2253 (2019). PubMed: 30450584
- Saxena S et al. ATR Signaling Uncouples the Role of RAD51 Paralogs in Homologous Recombination and Replication Stress Response. Cell Rep 29:551-559.e4 (2019). PubMed: 31618626
- Huang D et al. Isoorientin triggers apoptosis of hepatoblastoma by inducing DNA double-strand breaks and suppressing homologous recombination repair. Biomed Pharmacother 101:719-728 (2018). WB ; Human . PubMed: 29524880
- Saxena S et al. XRCC2 Regulates Replication Fork Progression during dNTP Alterations. Cell Rep 25:3273-3282.e6 (2018). PubMed: 30566856
- Wang H et al. Azidothymidine inhibits cell growth and telomerase activity and induces DNA damage in human esophageal cancer. Mol Med Rep 15:4055-4060 (2017). PubMed: 28487971
- Lagunas AM et al. Telomere DNA damage signaling regulates cancer stem cell evolution, epithelial mesenchymal transition, and metastasis. Oncotarget 8:80139-80155 (2017). PubMed: 29113290