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Anti-Chk2 (phospho T68) 抗体 (ab3501)

Reviews (2)Q&A (2)References (11)
Western blot - Anti-Chk2 (phospho T68) antibody (ab3501)

    Key features and details

    • Rabbit polyclonal to Chk2 (phospho T68)
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    製品の概要

    • 製品名

      Anti-Chk2 (phospho T68) antibody
      Chk2 一次抗体 製品一覧

    • 製品の詳細

      Rabbit polyclonal to Chk2 (phospho T68)

    • 由来種

      Rabbit

    • 特異性

      ab3501 was tested using MCF7 cells treated with different amounts of doxorubicin (a DNA damaging agent). As the treatment increased the WB signal at 60kDa representing Chk2 increased, indicating that ab3501 was detecting the phosphorylated form of the protein. It has also been shown by peptide ELISA to be specific for the phosphorylated form of the immunizing peptide versus the non-phosphorylated form.

      Reactivity occurs against human CHK2 pT68 protein and the antibody is specific for the phosphorylated form of the protein. Reactivity with non-phosphorylated human CHK2 is minimal by ELISA. The antibody does not cross-react with Chk2 phosphorylated at other sites. A BLAST analysis was used to suggest reactivity with this protein from human and chimpanzee based on 100% homology for the immunogen sequence. Cross reactivity with CHK2 protein from mouse and rat may occur as sequence homology varies by one amino acid residues in this sequence (90% homology). Cross reactivity with CHK2 homologues from other sources has not been determined.

    • アプリケーション

      適用あり: WBmore details

    • 種交差性

      交差種: Human
      交差が予測される動物種: Mouse, Rat, Chimpanzee

    • 免疫原

      Synthetic peptide corresponding to Chk2 aa 50-150 (phospho T68).

      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

    • 特記事項

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    製品の特性

    • 製品の状態

      Liquid

    • 保存方法

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    • バッファー

      Preservative: 0.01% Sodium azide
      Constituents: 0.424% Tripotassium orthophosphate, 0.88% Sodium chloride
    • Concentration information loading...

    • 精製度

      Immunogen affinity purified

    • 特記事項（精製）

      This affinity purified antibody is directed against the phosphorylated form of human CHK2 at the pT68 residue. The product was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross adsorbed against the non-phosphorylated form of the immunizing peptide.

    • ポリ/モノ

      ポリクローナル

    • アイソタイプ

      IgG

    • 研究分野

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab3501の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    WB (2)
    1/200 - 1/2000. Detects a band of approximately 61 kDa (predicted molecular weight: 61 kDa).
    特記事項
    WB
    1/200 - 1/2000. Detects a band of approximately 61 kDa (predicted molecular weight: 61 kDa).

    ターゲット情報

    • 機能

      Regulates cell cycle checkpoints and apoptosis in response to DNA damage, particularly to DNA double-strand breaks. Inhibits CDC25C phosphatase by phosphorylation on 'Ser-216', preventing the entry into mitosis. May also play a role in meiosis. Regulates the TP53 tumor suppressor through phosphorylation at 'Thr-18' and 'Ser-20'.

    • 組織特異性

      High expression is found in testis, spleen, colon and peripheral blood leukocytes. Low expression is found in other tissues.

    • 関連疾患

      Defects in CHEK2 are associated with Li-Fraumeni syndrome 2 (LFS2) [MIM:609265]; a highly penetrant familial cancer phenotype usually associated with inherited mutations in p53/TP53.
      Defects in CHEK2 may be a cause of susceptibility to prostate cancer (PC) [MIM:176807]. It is a malignancy originating in tissues of the prostate. Most prostate cancers are adenocarcinomas that develop in the acini of the prostatic ducts. Other rare histopathologic types of prostate cancer that occur in approximately 5% of patients include small cell carcinoma, mucinous carcinoma, prostatic ductal carcinoma, transitional cell carcinoma, squamous cell carcinoma, basal cell carcinoma, adenoid cystic carcinoma (basaloid), signet-ring cell carcinoma and neuroendocrine carcinoma.
      Defects in CHEK2 are found in some patients with osteogenic sarcoma (OSRC) [MIM:259500].

    • 配列類似性

      Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CHK2 subfamily.
      Contains 1 FHA domain.
      Contains 1 protein kinase domain.

    • 翻訳後修飾

      Phosphorylated by PLK4.

    • 細胞内局在

      Nucleus; Nucleus. Isoform 10 is present throughout the cell and Nucleus > PML body. Nucleus > nucleoplasm. Recruited into PML bodies together with TP53.
    • Information by UniProt

    • 参照データベース

      see all

    • 別名

      • CDS 1 antibody
      • Cds1 antibody
      • Cds1 homolog antibody
      • Checkpoint kinase 2 antibody
      • Checkpoint like protein CHK2 antibody
      • CHEK 2 antibody
      • Chek2 antibody
      • Chk 2 antibody
      • CHK2 checkpoint homolog (S. pombe) antibody
      • CHK2 checkpoint homolog antibody
      • CHK2_HUMAN antibody
      • hCds1 antibody
      • HuCds 1 antibody
      • LFS 2 antibody
      • LFS2 antibody
      • PP1425 antibody
      • RAD 53 antibody
      • RAD53 antibody
      • Rad53 homolog antibody
      • Serine/threonine protein kinase Chk2 antibody
      • Serine/threonine-protein kinase Chk2 antibody
      see all

    画像

    • Western blot - Anti-Chk2 (phospho T68) antibody (ab3501)
      Western blot - Anti-Chk2 (phospho T68) antibody (ab3501)

      The Western blot shows lysates from MCF-7 cells treated for 24 hours with increasing concentrations of the DNA damaging agent Doxorubicin (0-10µM). The blot clearly shows that Chk2 phosphorylation is increased with the higher concentrations of Doxorubicin.

    参考文献 (11)

    ab3501 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab3501 は 11 報の論文で使用されています。

    • Einig E  et al. RNAPII-dependent ATM signaling at collisions with replication forks. Nat Commun 14:5147 (2023). PubMed: 37620345
    • Lan B  et al. CRISPR-Cas9 Screen Identifies DYRK1A as a Target for Radiotherapy Sensitization in Pancreatic Cancer. Cancers (Basel) 14:N/A (2022). PubMed: 35053488
    • Dunlop CR  et al. Complete loss of ATM function augments replication catastrophe induced by ATR inhibition and gemcitabine in pancreatic cancer models. Br J Cancer 123:1424-1436 (2020). PubMed: 32741974
    • Wang J  et al. Bacterial quorum-sensing signal IQS induces host cell apoptosis by targeting POT1-p53 signalling pathway. Cell Microbiol 21:e13076 (2019). PubMed: 31254473
    • Pan Z  et al. Cinobufagin Induces Cell Cycle Arrest at the G2/M Phase and Promotes Apoptosis in Malignant Melanoma Cells. Front Oncol 9:853 (2019). PubMed: 31552178
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-4 of 4 Abreviews or Q&A

    Western blot abreview for Anti-Chk2 (phospho T68) antibody

     Good
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (mouse prostate cells)
    Gel Running Conditions
    Reduced Denaturing (4-12% Bis-Tris)
    Loading amount
    20 µg
    Treatment
    10 Gy irradiation
    Specification
    mouse prostate cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Jan 11 2021

    Western blot abreview for Anti-Chk2 (phospho T68) antibody

     Inconclusive
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    25 µg
    Gel Running Conditions
    Reduced Denaturing (10%)
    Sample
    Mouse Cell lysate - whole cell (Neural stem cells)
    Specification
    Neural stem cells
    Treatment
    MMS 0,02% Adriamicine 0,5 ´g /mL
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. J Igual

    Verified customer

    投稿 May 08 2014

    Question


    I have been looking for a phospho-Chk2 (Thr68) antibody that works on material from rats or mice, for use in WB or IHC. I see on your website that you have 3 antibodies , no.s’ ab38461, ab85743 and ab3501 in your catalogue, and I just wondered if you could tell me whether they would work in rodent tissue? I know the latter 2 are ‘predicted’ to work in rat and mouse, but I wondered if anyone had commented saying that this has been confirmed?

    If this remains unknown, would it be possible to one of the antibodies at a discounted rate, and I could test it for you, to see if it does work?

    Many thanks

    Read More
    Answer

    Thank you very much for your interest in our antibodies against Chk2 phospho Thr68.
    To our knowledge, all these three antibodies have not been tested in rodent species. All these three antibodies are however predicted to react against the mouse and the rat protein, as the sequence around the Thr68 is quite conserved. Please see here the alignment:
    http://www.ebi.ac.uk/Tools/services/web_clustalw2/toolresult.ebi?tool=clustalw2&jobId=clustalw2-I20120420-160419-0159-55604608-oy
    The crossreaction needs however to be tested experimentally and I can make you an interesting offer if you would like to test this: I can offer a discount off a future purchase if you buy one of these antibodies now, test it in rodent species and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of: 1 free primary antibody.
    If you would like to test one of these antibodies, I would recommend to test the ab38461, as it is the only of these three antibodies which has been tested in Western blot and IHC-P. I leave this decision however up to you, and I am happy to provide the code for testing also another one of these three antibodies on rat and mouse.
    If you are interested in this offer, please follow these steps:
    1. Reply to this e-mail to let me know which antibody you would like to test in rat and mouse. I will then send a discount code. This code must be issued before purchasing the antibody so please wait for my reply before ordering.
    2. Purchase the antibody either by phone, fax, or online (www.abcam.com).
    3. Test it in mouse and rat.
    4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.
    5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue.
    We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if the anti Chk2 phospho Thr68 turns out to be unsuitable for rat or mouse, you will still receive the discount on your next purchase after your Abreview has been submitted.
    Please let me know if you have any questions about this offer and I would be happy to help you further.
    The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

    Read More

    Question

    I am looking for a phosphorylated checkpoint kinase 2 antibody for mouse at threonine 68. On the data sheet of ab3501 it stated it was specific for humans and based on 90% homology may react with mouse. I was wondering if you had any feedback that this antibody does react with mouse samples.

    Read More
    Answer

    Thank you for your enquiry. We have not received any feedback from customers who have tried this antibody for cross-reactivity with mouse. Although it is predicted to cross-react due to 90% sequence homology, we cannot guarantee it. Should you decide to go ahead and purchase this product, please let us know how you get on by submitting an Abreview and in return we will award you 50 Abcam Points, which can be redeemed on a number of rewards (a further 100 points will be offered for an image). Please let me know if you require any further assistance.

    Read More

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