Anti-Chk1 (phospho S345) 抗体 (ab58567)
Key features and details
- Rabbit polyclonal to Chk1 (phospho S345)
- Suitable for: WB, IHC-P, ELISA
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Chk1 (phospho S345) antibody
Chk1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Chk1 (phospho S345) -
由来種
Rabbit -
特異性
Specific for Chk1 (phospho S345) in ELISA, with <1% reactivity with the non-modified form of the immunogen. -
アプリケーション
適用あり: WB, IHC-P, ELISAmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Chicken, Cow
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- This antibody gave a positive signal in Hydroxyurea Treated (48hr, 2uM) Hela whole cell lysate. IHC-P: FFPE Human cervix normal.
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特記事項
Please note we cannot guarantee ICC/IF, however should you use this application for this product then please use a specific protocol which can be found in the protocols section of our datasheet here.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Control Peptide
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab58567の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (4) |
Use a concentration of 1 µg/ml. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).
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| IHC-P |
Use a concentration of 1 - 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| ELISA |
Use at an assay dependent concentration.
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| 特記事項 |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa). |
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IHC-P
Use a concentration of 1 - 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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ELISA
Use at an assay dependent concentration. |
ターゲット情報
- Information by UniProt
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参照データベース
- Entrez Gene: 513678 Cow
- Entrez Gene: 1111 Human
- Entrez Gene: 12649 Mouse
- Entrez Gene: 140583 Rat
- Omim: 603078 Human
- SwissProt: O14757 Human
- SwissProt: O35280 Mouse
- SwissProt: Q91ZN7 Rat
see all -
別名
- C85740 antibody
- Cell cycle checkpoint kinase antibody
- Checkpoint , S. pombe, homolog of, 1 antibody
see all
画像
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ELISA - Anti-Chk1 (phospho S345) antibody (ab58567)
ELISA using ab58567 at varying antibody concentrations. Curve_SPL5 indicates binding to the Chk1 (phospho S345) peptide. Binding to the following peptides was not seen: Chk1 control peptide (Curve_SPL6).
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Western blot - Anti-Chk1 (phospho S345) antibody (ab58567)All lanes : Anti-Chk1 (phospho S345) antibody (ab58567) at 1 µg/ml
Lane 1 : HeLa Whole Cell Lysate - Untreated
Lane 2 : HeLa Whole Cell Lysate - Hydroxyurea Treated (48hr, 2uM)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 54 kDa
Observed band size: 54 kDa
Additional bands at: 105 kDa, 42 kDa, 90 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab58567 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chk1 (phospho S345) antibody (ab58567)IHC image of ab58567 staining Human Cervical Carcinoma formalin fixed paraffin embedded tissue section*, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab58567, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (18)
ab58567 は 18 報の論文で使用されています。
- Hao X et al. Disrupted mitochondrial homeostasis coupled with mitotic arrest generates antineoplastic oxidative stress. Oncogene 41:427-443 (2022). PubMed: 34773075
- Niwa Y et al. Cyclin D1 Binding Protein 1 Responds to DNA Damage through the ATM-CHK2 Pathway. J Clin Med 11:N/A (2022). PubMed: 35160302
- Frittoli E et al. Tissue fluidification promotes a cGAS-STING cytosolic DNA response in invasive breast cancer. Nat Mater N/A:N/A (2022). PubMed: 36581770
- Llerena Cari E et al. Integrated stress response control of granulosa cell translation and proliferation during normal ovarian follicle development. Mol Hum Reprod 27:N/A (2021). PubMed: 34314477
- Napoletano F et al. The prolyl-isomerase PIN1 is essential for nuclear Lamin-B structure and function and protects heterochromatin under mechanical stress. Cell Rep 36:109694 (2021). PubMed: 34525372