Anti-Chk1 (phospho S317) 抗体 (ab38518)
Key features and details
- Rabbit polyclonal to Chk1 (phospho S317)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
-
製品名
Anti-Chk1 (phospho S317) antibody
Chk1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Chk1 (phospho S317) -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to Human Chk1 aa 250-350 (phospho S317).
Database link: O14757 -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, PBS
Without Mg2+ and Ca2+ -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
ab38518 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab38518の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (2) |
1/500 - 1/1000. Detects a band of approximately 63 kDa (predicted molecular weight: 54 kDa).
|
| 特記事項 |
|---|
|
WB
1/500 - 1/1000. Detects a band of approximately 63 kDa (predicted molecular weight: 54 kDa). |
ターゲット情報
- Information by UniProt
-
参照データベース
- Entrez Gene: 1111 Human
- Omim: 603078 Human
- SwissProt: O14757 Human
- Unigene: 24529 Human
-
別名
- C85740 antibody
- Cell cycle checkpoint kinase antibody
- Checkpoint , S. pombe, homolog of, 1 antibody
see all
画像
-
Western blot - Anti-Chk1 (phospho S317) antibody (ab38518)All lanes : Anti-Chk1 (phospho S317) antibody (ab38518) at 1/500 dilution
Lane 1 : Extracts from MCF7 cells (5-30ug of total protein)
Lane 2 : Extracts from COLO cells (5-30ug of total protein)
Secondary
All lanes : Alkaline Phosphatase Goat Anti Rabbit IgG (H+L)
Predicted band size: 54 kDa
Observed band size: 63 kDa why is the actual band size different from the predicted?
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (2)
ab38518 は 2 報の論文で使用されています。
- Jiang D et al. TET3-mediated DNA oxidation promotes ATR-dependent DNA damage response. EMBO Rep 18:781-796 (2017). PubMed: 28325772
- Kadaja M et al. Mechanism of genomic instability in cells infected with the high-risk human papillomaviruses. PLoS Pathog 5:e1000397 (2009). ICC/IF ; Human . PubMed: 19390600