Anti-CHD8 抗体 (ab84527)
Key features and details
- Rabbit polyclonal to CHD8
- Suitable for: IHC-P, WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-CHD8 antibody
CHD8 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to CHD8 -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, WB, IPmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rabbit, Horse, Guinea pig, Cow, Pig, Chimpanzee, Monkey, Rhesus monkey, Gorilla, Orangutan, Elephant
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免疫原
Synthetic peptide within Human CHD8 aa 2250-2350. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. NP_065971.2 (GeneID 57680).
Database link: Q9HCK8 -
ポジティブ･コントロール
- IHC-P: Human breast carcinoma and prostate carcinoma tissue.
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特記事項
Concentration is optimized for IHC and not determined
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab84527の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| WB |
Use at an assay dependent concentration. Predicted molecular weight: 291 kDa. PubMed: 24705355
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| IP |
Use at an assay dependent concentration. PubMed: 23285124
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| 特記事項 |
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IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 291 kDa. PubMed: 24705355 |
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IP
Use at an assay dependent concentration. PubMed: 23285124 |
ターゲット情報
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機能
DNA helicase that acts as a chromatin remodeling factor and regulates transcription. Acts as a transcription repressor by remodeling chromatin structure and recruiting histone H1 to target genes. Suppresses p53/TP53-mediated apoptosis by recruiting histone H1 and preventing p53/TP53 transactivation activity. Acts as a negative regulator of Wnt signaling pathway by regulating beta-catenin (CTNNB1) activity. Negatively regulates CTNNB1-targeted gene expression by being recruited specifically to the promoter regions of several CTNNB1 responsive genes. Involved in both enhancer blocking and epigenetic remodeling at chromatin boundary via its interaction with CTCF. Acts as a suppressor of STAT3 activity by suppressing the LIF-induced STAT3 transcriptional activity. Also acts as a transcription activator via its interaction with ZNF143 by participating to efficient U6 RNA polymerase III transcription. -
配列類似性
Belongs to the SNF2/RAD54 helicase family. CHD8 subfamily.
Contains 2 chromo domains.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain. -
翻訳後修飾
Sumoylated. -
細胞内局在
Nucleus. Localizes to the promoter regions of several CTNNB1-responsive genes. Also present at known CTCF target sites. - Information by UniProt
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参照データベース
- Entrez Gene: 57680 Human
- Entrez Gene: 67772 Mouse
- Omim: 610528 Human
- SwissProt: Q9HCK8 Human
- SwissProt: Q09XV5 Mouse
- Unigene: 530698 Human
- Unigene: 289934 Mouse
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別名
- ATP dependent helicase CHD8 antibody
- ATP-dependent helicase CHD8 antibody
- Axis duplication inhibitor antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CHD8 antibody (ab84527)ab84527, at 1/100 dilution, staining CHD8 in formalin-fixed, paraffin-embedded human breast carcinoma by Immunohistochemistry, using DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CHD8 antibody (ab84527)ab84527, at 1/100 dilution, staining CHD8 in formalin-fixed, paraffin-embedded human prostate carcinoma by Immunohistochemistry using DAB staining.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (10)
ab84527 は 10 報の論文で使用されています。
- Pintacuda G et al. Protein interaction studies in human induced neurons indicate convergent biology underlying autism spectrum disorders. Cell Genom 3:100250 (2023). PubMed: 36950384
- Chatterjee I et al. CHD8 regulates gut epithelial cell function and affects autism-related behaviors through the gut-brain axis. Transl Psychiatry 13:305 (2023). PubMed: 37783686
- Dong C et al. Conserved and Distinct Functions of the Autism-Related Chromatin Remodeler CHD8 in Embryonic and Adult Forebrain Neurogenesis. J Neurosci 42:8373-8392 (2022). PubMed: 36127134
- Kweon H et al. Excitatory neuronal CHD8 in the regulation of neocortical development and sensory-motor behaviors. Cell Rep 34:108780 (2021). PubMed: 33626347
- Jiménez JA et al. Chd8 haploinsufficiency impairs early brain development and protein homeostasis later in life. Mol Autism 11:74 (2020). PubMed: 33023670
- Zhao C et al. Dual Requirement of CHD8 for Chromatin Landscape Establishment and Histone Methyltransferase Recruitment to Promote CNS Myelination and Repair. Dev Cell 45:753-768.e8 (2018). PubMed: 29920279
- Akhade VS et al. Mechanism of Wnt signaling induced down regulation of mrhl long non-coding RNA in mouse spermatogonial cells. Nucleic Acids Res 44:387-401 (2016). PubMed: 26446991
- Tahara T et al. Colorectal carcinomas with CpG island methylator phenotype 1 frequently contain mutations in chromatin regulators. Gastroenterology 146:530-538.e5 (2014). IHC-P ; Human . PubMed: 24211491
- Schulz Y et al. CHARGE and Kabuki syndromes: a phenotypic and molecular link. Hum Mol Genet N/A:N/A (2014). WB ; Human . PubMed: 24705355
- Batsukh T et al. Identification and characterization of FAM124B as a novel component of a CHD7 and CHD8 containing complex. PLoS One 7:e52640 (2012). IP ; Human . PubMed: 23285124