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Anti-CHD6 抗体 [2174C2a] (ab51330)

Reviews (1) Submit a question References (1)
Western blot - Anti-CHD6 antibody [2174C2a] (ab51330)

    Key features and details

    • Mouse monoclonal [2174C2a] to CHD6
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG1

    製品の概要

    • 製品名

      Anti-CHD6 antibody [2174C2a]
      CHD6 一次抗体 製品一覧

    • 製品の詳細

      Mouse monoclonal [2174C2a] to CHD6

    • 由来種

      Mouse

    • アプリケーション

      適用あり: WBmore details

    • 種交差性

      交差種: Human

    • 免疫原

      Recombinant human CHD6 fragment

    • ポジティブ･コントロール

      • HeLa whole cell lysate

    • 特記事項

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    製品の特性

    • 製品の状態

      Liquid

    • 保存方法

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.

    • バッファー

      pH: 7.40
      Preservative: 0.05% Sodium azide
      Constituents: 0.812% Sodium chloride, 0.1312% Sodium phosphate, 0.0225% Potassium chloride, 1% BSA, 0.03% Potassium phosphate, PBS
    • Concentration information loading...

    • 精製度

      Protein G purified

    • ポリ/モノ

      モノクローナル

    • クローン名

      2174C2a

    • アイソタイプ

      IgG1

    • 研究分野

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab51330の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    WB (1)
    1/50. Predicted molecular weight: 305 kDa.
    特記事項
    WB
    1/50. Predicted molecular weight: 305 kDa.

    ターゲット情報

    • 機能

      Probable transcription regulator.

    • 組織特異性

      Ubiquitous.

    • 配列類似性

      Belongs to the SNF2/RAD54 helicase family.
      Contains 2 chromo domains.
      Contains 1 helicase ATP-binding domain.
      Contains 1 helicase C-terminal domain.

    • 翻訳後修飾

      Phosphorylated upon DNA damage, probably by ATM or ATR.

    • 細胞内局在

      Nucleus.
    • Information by UniProt

    • 参照データベース

      • 別名

        • ATP dependent helicase CHD6 antibody
        • ATP-dependent helicase CHD6 antibody
        • CHD-6 antibody
        • CHD5 antibody
        • CHD6 antibody
        • CHD6_HUMAN antibody
        • chromodomain helicase DNA binding protein 6 antibody
        • Chromodomain-helicase-DNA-binding protein 6 antibody
        • helicase C-terminal domain- and SNF2 N-terminal domain-containing protein antibody
        • KIAA1335 antibody
        • Radiation induced gene B protein antibody
        • Radiation-induced gene B protein antibody
        • RIGB antibody
        • RP4-620E11.2 antibody
        see all

      画像

      • Western blot - Anti-CHD6 antibody [2174C2a] (ab51330)
        Western blot - Anti-CHD6 antibody [2174C2a] (ab51330)
        Anti-CHD6 antibody [2174C2a] (ab51330) at 1/50 dilution + HeLa whole cell lysate at 25 µg

        Secondary
        anti-mouse IgG at 1/2500 dilution

        Developed using the ECL technique.

        Predicted band size: 305 kDa
        Observed band size: 305 kDa

      参考文献 (1)

      ab51330 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab51330 は 1 報の論文で使用されています。

      • Alfonso R  et al. CHD6 chromatin remodeler is a negative modulator of influenza virus replication that relocates to inactive chromatin upon infection. Cell Microbiol 13:1894-906 (2011). PubMed: 21899694

      レビューと Q&A

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      レビューを送る 質問を送る

      Western blot abreview for Anti-CHD6 antibody [2174C2a]

       Poor
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Mouse Cell lysate - nuclear (mouse and human cell lines)
      Gel Running Conditions
      Reduced Denaturing
      Loading amount
      20 µg
      Specification
      mouse and human cell lines
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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      Verified customer

      投稿 Apr 20 2016

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