Anti-CHD1L 抗体 [2170C3a] (ab51324)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CHD1L antibody [2170C3a] (ab51324)](https://www.abcam.co.jp/ps/products/51/ab51324/Images/ab51324-63353-anti-chd1l-antibody-2170c3a-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CHD1L antibody [2170C3a] (ab51324)")
Key features and details
- Mouse monoclonal [2170C3a] to CHD1L
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
- Isotype: IgG1
製品の概要
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製品名
Anti-CHD1L antibody [2170C3a]
CHD1L 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [2170C3a] to CHD1L -
由来種
Mouse -
アプリケーション
適用あり: WB, IP, IHC-Pmore details -
種交差性
交差種: Human -
免疫原
Recombinant human CHD1L fragment
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ポジティブ･コントロール
- HEK293 whole cell lysate.
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特記事項
This product was changed from ascites to tissue culture supernatant on 9th August 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
バッファー
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 1% BSA, 0.03% Potassium phosphate, 0.812% Sodium chloride, 0.1312% Sodium phosphate, 0.0225% Potassium chloride, PBS -
Concentration information loading... -
精製度
Protein G purified -
特記事項（精製）
Purified from TCS -
ポリ/モノ
モノクローナル -
クローン名
2170C3a -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab51324の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (2) | |
| IP | ||
| IHC-P |
| 特記事項 |
|---|
追加情報
Dot: Use at an assay dependent dilution.
IHC-P: Use at an assay dependent dilution (PMID 20335658).
IP: Use at 10µg/mg RIPA lysate of HeLa cells.
WB: 1/50. Detects a band of approximately 101 kDa (predicted molecular weight: 101 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use at an assay dependent dilution (PMID 20335658).
IP: Use at 10µg/mg RIPA lysate of HeLa cells.
WB: 1/50. Detects a band of approximately 101 kDa (predicted molecular weight: 101 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
ターゲット情報
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機能
DNA helicase which plays a role in chromatin-remodeling following DNA damage. Targeted to sites of DNA damage through interaction with poly(ADP-ribose) and functions to regulate chromatin during DNA repair. Able to catalyze nucleosome sliding in an ATP-dependent manner. Helicase activity is strongly stimulated upon poly(ADP-ribose)-binding. -
組織特異性
Frequently overexpressed in hepatomacellular carcinomas. -
配列類似性
Belongs to the SNF2/RAD54 helicase family.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
Contains 1 Macro domain. -
ドメイン
The macro domain mediates non-covalent poly(ADP-ribose)-binding and recruitment to DNA damage sites. -
細胞内局在
Nucleus. Localizes at sites of DNA damage. Probably recruited to DNA damage sites by PARylated PARP1. - Information by UniProt
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参照データベース
- Entrez Gene: 9557 Human
- Omim: 613039 Human
- SwissProt: Q86WJ1 Human
- Unigene: 191164 Human
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別名
- ALC1 antibody
- Amplified in liver cancer 1 antibody
- Amplified in liver cancer protein 1 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CHD1L antibody [2170C3a] (ab51324)IHC image of ab51324 staining in human kidney formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab51324, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. -
![Western blot - Anti-CHD1L antibody [2170C3a] (ab51324)](https://www.abcam.co.jp/ps/products/51/ab51324/Images/ab51324-63355-anti-chd1l-antibody-2170c3a-western-blot.gif)
Western blot - Anti-CHD1L antibody [2170C3a] (ab51324)Anti-CHD1L antibody [2170C3a] (ab51324) at 1/50 dilution + HEK293 whole cell lysate at 25 µg
Secondary
anti-mouse IgG at 1/2500 dilution
Developed using the ECL technique.
Predicted band size: 101 kDa
Observed band size: 101 kDa -
![Immunoprecipitation - Anti-CHD1L antibody [2170C3a] (ab51324)](https://www.abcam.co.jp/ps/products/51/ab51324/Images/ab51324-63354-anti-chd1l-antibody-2170c3a-immunoprecipitation.gif)
Immunoprecipitation - Anti-CHD1L antibody [2170C3a] (ab51324)Immunoprecipitation analysis using ab51324 at 5ug/500ug RIPA lysate of HeLa cells. Immunoblot uisng ab51324 at 1/50 dilution and 1/2500 anti-mouse IgG as secondary.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (8)
ab51324 は 8 報の論文で使用されています。
- Ding X et al. PARP1-SNAI2 transcription axis drives resistance to PARP inhibitor, Talazoparib. Sci Rep 12:12501 (2022). PubMed: 35864202
- Wang G et al. CHD1L prevents lipopolysaccharide-induced hepatocellular carcinomar cell death by activating hnRNP A2/B1-nmMYLK axis. Cell Death Dis 12:891 (2021). PubMed: 34588420
- Blessing C et al. The Oncogenic Helicase ALC1 Regulates PARP Inhibitor Potency by Trapping PARP2 at DNA Breaks. Mol Cell 80:862-875.e6 (2020). PubMed: 33275888
- Tsuda M et al. ALC1/CHD1L, a chromatin-remodeling enzyme, is required for efficient base excision repair. PLoS One 12:e0188320 (2017). PubMed: 29149203
- Oza J et al. A Novel Role of Chromodomain Protein CBX8 in DNA Damage Response. J Biol Chem 291:22881-22893 (2016). PubMed: 27555324
- Hyeon J et al. CHD1L Is a Marker for Poor Prognosis of Hepatocellular Carcinoma after Surgical Resection. Korean J Pathol 47:9-15 (2013). IHC . PubMed: 23482400
- Chen L et al. Clinical significance of CHD1L in hepatocellular carcinoma and therapeutic potentials of virus-mediated CHD1L depletion. Gut 60:534-43 (2011). WB ; Human . PubMed: 21068133
- Chen L et al. CHD1L promotes hepatocellular carcinoma progression and metastasis in mice and is associated with these processes in human patients. J Clin Invest 120:1178-91 (2010). IHC-P ; Mouse . PubMed: 20335658
