Anti-CGRP 抗体 [4901] (ab81887)
![Immunocytochemistry/ Immunofluorescence - Anti-CGRP antibody [4901] (ab81887)](https://www.abcam.co.jp/ps/products/81/ab81887/Images/ab81887-96924-anti-cgrp-antibody-4901-immunofluorescence.gif "Immunocytochemistry/ Immunofluorescence - Anti-CGRP antibody [4901] (ab81887)")
Key features and details
- Mouse monoclonal [4901] to CGRP
- Suitable for: ICC/IF, IHC-Fr, Flow Cyt
- Reacts with: Mouse, Rat, Guinea pig, Human
- Isotype: IgG2a
製品の概要
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製品名
Anti-CGRP antibody [4901]
CGRP 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [4901] to CGRP -
由来種
Mouse -
特異性
ab81887 is specific for Calcitonin Gene Related Peptide. It also recognizes rat beta CGRP and human alpha CGRP (10 fold less sensitivity compared to rat alpha CGRP). -
アプリケーション
適用あり: ICC/IF, IHC-Fr, Flow Cytmore details
適用なし: WB -
種交差性
交差種: Mouse, Rat, Guinea pig, Human
交差が予測される動物種: Mammals
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免疫原
Full length protein corresponding to Rat CGRP aa 1 to the C-terminus.
Database link: P01256 -
ポジティブ･コントロール
- Dorsal root ganglia
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.50
Constituents: 0.01% BSA, 50% Glycerol, 0.87% Sodium chloride, 0.238% HEPES -
Concentration information loading... -
精製度
Protein G purified -
一次抗体 備考
This antibody is an excellent tool for immunohistochemistry and for multiple labeling immunofluorescence of peripheral tissue like stomach, intestine, pancreas, sensory ganglia, and all the regions of the body that are innervated by primary afferent neurons as well as central nervous tissue. -
ポリ/モノ
モノクローナル -
クローン名
4901 -
アイソタイプ
IgG2a -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab81887の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF | (1) |
1/50 - 1/100.
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| IHC-Fr | (4) |
Use at an assay dependent concentration.
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| Flow Cyt |
Use at an assay dependent concentration. PubMed: 24430801
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
| 特記事項 |
|---|
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ICC/IF
1/50 - 1/100. |
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IHC-Fr
Use at an assay dependent concentration. |
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Flow Cyt
Use at an assay dependent concentration. PubMed: 24430801 ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
追加情報
Is unsuitable for WB.
ターゲット情報
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関連性
CGRP is present in C-cells of the thyroid and in central and peripheral nerves. CGRP has several important physiologic roles: it is a potent vasodilator, and can affect the force and rate of heart beat; it can modulate acetylcholine receptor function at the neuromuscular junction; it has been demonstrated to block tolerance to morphine; and it can modulate antigen presentation in Langerhans cells in the skin. -
細胞内局在
Endoplasmic reticulum and Secreted -
参照データベース
- Entrez Gene: 796 Human
- Entrez Gene: 797 Human
- Entrez Gene: 116903 Mouse
- Entrez Gene: 12310 Mouse
- Entrez Gene: 171519 Rat
- Entrez Gene: 24241 Rat
- Omim: 114130 Human
- Omim: 114160 Human
see all -
別名
- Calcitonin/calcitonin related polypeptide alpha antibody
- Alpha type CGRP antibody
- Beta type CGRP antibody
see all
画像
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Immunocytochemistry/ Immunofluorescence - Anti-CGRP antibody [4901] (ab81887)Immunofluorescent staining of CGRP (red) in cryopreserved DRG neurons grown on coverslips using ab81887 at 1/50 dilution. Green staining is PGP9.5. Yellow staining is the colocalization of CGRP and PGP9.5.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (153)
ab81887 は 153 報の論文で使用されています。
- Wu H et al. Temporal alterations of pituitary adenylate cyclase activating polypeptide and its receptors in a rat model induced by recurrent chemical stimulations: Relevant to chronic migraine. Mol Pain 19:17448069231152129 (2023). PubMed: 36604785
- Belinskaia M et al. Bipartite Activation of Sensory Neurons by a TRPA1 Agonist Allyl Isothiocyanate Is Reflected by Complex Ca2+ Influx and CGRP Release Patterns: Enhancement by NGF and Inhibition with VAMP and SNAP-25 Cleaving Botulinum Neurotoxins. Int J Mol Sci 24:N/A (2023). PubMed: 36674850
- Yang OJ et al. Evaluating the transduction efficiency of systemically delivered AAV vectors in the rat nervous system. Front Neurosci 17:1001007 (2023). PubMed: 36755734
- Arthurs JW et al. Activation of Parabrachial Tachykinin 1 Neurons Counteracts Some Behaviors Mediated by Parabrachial Calcitonin Gene-related Peptide Neurons. Neuroscience 517:105-116 (2023). PubMed: 36898496
- Nimbalkar S et al. Development of a functional human induced pluripotent stem cell-derived nociceptor MEA system as a pain model for analgesic drug testing. Front Cell Dev Biol 11:1011145 (2023). PubMed: 36936691