Anti-cGAS 抗体 [EPR26492-84] - BSA and Azide free (ab302618)

This data was developed using ab302617, the same antibody clone in a different buffer formulation.

Blocking / Diluting buffer and concentration: 5% NFDM/TBST

Lysate was freshly made and used for Western blotting immediately to minimize protein degradation.
The identity of the bands above 250 kDa are unknown.

This data was developed using ab302617, the same antibody clone in a different buffer formulation.

Blocking / Diluting buffer and concentration: 5% NFDM/TBST

Negative control: HCT 116 (PMID: 33790360)
HT-29 lysate was freshly made and used for Western blotting immediately to minimize protein degradation.
The bands between 40 kDa and 23 kDa are likely to be degraded target fragments.

This data was developed using ab302617, the same antibody clone in a different buffer formulation.

Blocking / Diluting buffer and concentration: 5% NFDM/TBST

Lysate was freshly made and used for Western blotting immediately to minimize protein degradation.
The band beneath the target band (23 kDa) is likely to be degraded target fragment.

This data was developed using ab302617, the same antibody clone in a different buffer formulation.

Blocking / Diluting buffer and concentration: 5% NFDM/TBST

Lysate was freshly made and used for Western blotting immediately to minimize protein degradation.
The identity of the higher MW band at approximately 250 kDa is unknown.

This data was developed using ab302617, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized No-GFP-CD16.NK-92 (human lymphoblast natural killer cell; nk cell) cells labeling cGAS with ab302617 at 1/50 (10.0 µg/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed antibody at 1/1000 (2µg/ml) dilution (Green). Confocal image showing cytoplasmic and membranous staining in No-GFP-CD16.NK-92 cell line. Negative control: HCT 116 (PMID: 33790360) was used to counterstain tubulin at 1/200 2.5 dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 (2µg/ml) dilution.

This data was developed using ab302617, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HCT 116 (human colorectal carcinoma epithelial cell, Left) / No-GFP-CD16.NK-92 (human malignant non-Hodgkin's lymphoma natural killer cell, Right) cells labeling cGAS with ab302617 at 1/500 dilution (0.1µg) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor^® 488, ab150081) at 1/2000 dilution was used as the secondary antibody. Negative control: HCT 116 (PMID: 33790360)

This data was developed using ab302617, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HT-29 (Human colorectal adenocarcinoma epithelial cell) cells labeling cGAS with ab302617 at 1/500 dilution (0.1µg) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor^® 488, ab150081) at 1/2000 dilution was used as the secondary antibody.

This data was developed using ab302617, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized MCF7 (human breast adenocarcinoma epithelial cell) cells labeling cGAS with ab302617 at 1/500 dilution (0.1µg) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor^® 488, ab150081) at 1/2000 dilution was used as the secondary antibody.