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AB92738

Anti-CGA/hCG alpha 抗体 [EP3373]

Anti-CGA/hCG alpha antibody [EP3373]

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(2 Publications)

Rabbit Recombinant Monoclonal GLHA antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 2 publications.

別名を表示する

Glycoprotein hormones alpha chain, Anterior pituitary glycoprotein hormones common subunit alpha, Choriogonadotropin alpha chain, Chorionic gonadotrophin subunit alpha, Follicle-stimulating hormone alpha chain, Follitropin alpha chain, Luteinizing hormone alpha chain, Lutropin alpha chain, Thyroid-stimulating hormone alpha chain, Thyrotropin alpha chain, CG-alpha, FSH-alpha, LSH-alpha, TSH-alpha, CGA

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CGA/hCG alpha antibody [EP3373] (AB92738)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CGA/hCG alpha antibody [EP3373] (AB92738)

Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling CGA/hCG alpha with ab92738 at 1/600 dilution (0.87 µg/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Western blot - Anti-CGA/hCG alpha antibody [EP3373] (AB92738)
  • WB

Lab

Western blot - Anti-CGA/hCG alpha antibody [EP3373] (AB92738)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Lanes 1 - 2:

Western blot - Anti-CGA/hCG alpha antibody [EP3373] (ab92738) at 1/2000 dilution

Lane 1:

Western blot - Anti-PKR antibody [EPR19374] (<a href='/products/primary-antibodies/pkr-antibody-epr19374-ab184257'>ab184257</a>) at 1/1000 dilution

Lane 1:

JAR (Human placenta choriocarcinoma epithelial cell) whole cell lysate

Lane 1:

bEnd.3 (mouse brain endothelial cell) whole cell lysate at 20 µg

Lane 2:

JAR (Human placenta choriocarcinoma epithelial cell) whole cell lysate treated with Hydroxyurea (10mM 24h)

Secondary

Lanes 1 - 2:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Lane 1:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 18 kDa

false

Exposure time: 180s

関連する標識済み抗体及び組成の異なる製品 (1)

  • Carrier free

    Anti-CGA/hCG alpha antibody [EP3373] - BSA and Azide free

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EP3373

アイソタイプ

IgG

キャリアフリー

No

交差種

Human

アプリケーション

WB, IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/600", "IHCP-species-notes": "<p><strong>For unpurified, use 1/100 - 1/250.</strong></p>" } } }
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製品の詳細

This product has switched from a hybridoma to recombinant production method on 2nd August 2023.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Stable for 12 months at -20°C
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

CGA also known as the hCG alpha subunit is a glycoprotein hormone free alpha subunit. Its molecular mass is approximately 15-22 kDa. It is a part of the human chorionic gonadotropin (hCG) protein. It is expressed in the placenta and various neoplastic tissues. CGA along with the beta subunit forms the complete structure of hCG which plays a significant role in signaling for the reproductive processes.
Biological function summary

This alpha subunit plays a fundamental role in forming the heterodimer with different beta subunits of hCG TSH LH and FSH resulting in various glycoprotein hormones. These hormones are essential for maintaining pregnancy fetal development and regulating the menstrual cycle. The CGA protein is key to the activity of the hCG protein which significantly impacts fetal and trophoblastic growth during pregnancy. The heterodimeric complex to which it contributes assigns it unique biological roles that differ depending on the linked beta subunit.

Pathways

The CGA subunit functions within the reproductive hormone signaling pathway. The uniform alpha subunit pairs with distinct beta subunits such as LH beta or FSH beta influencing different signaling pathways. It plays a vital part in the hormonal regulation pathway modulating endocrine and reproductive processes. The related proteins include the beta subunits like hCG beta LH beta and FSH beta which pair with CGA to perform distinct biological functions in different tissues.

CGA's expression relates to trophoblastic diseases and certain types of cancer such as choriocarcinoma. Aberrant expression or regulation can be seen in these conditions linking it to tumorigenesis and abnormal reproductive disorders. Choriocarcinoma significantly involves hCG protein and its subunits where CGA influences the progression by contributing to this hormone's abnormal activity. Elevated levels of CGA can correlate with the progression of these diseases serving as a clinical marker for diagnosis.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Shared alpha chain of the active heterodimeric glycoprotein hormones thyrotropin/thyroid stimulating hormone/TSH, lutropin/luteinizing hormone/LH, follitropin/follicle stimulating hormone/FSH and choriogonadotropin/CG. These hormones bind specific receptors on target cells that in turn activate downstream signaling pathways.
See full target information CGA
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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Annals of medicine and surgery (2012) 86:2644-2650 PubMed38694326

2024

S100A6 could not promote the differentiation of Calu-6 lung cancer cell line.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Li,Ting Wang,Dan Dang

Pituitary 18:31-41 PubMed24402129

2014

Expression of the pituitary stem/progenitor marker GFRα2 in human pituitary adenomas and normal pituitary.

Applications

Unspecified application

Species

Unspecified reactive species

Nestoras Mathioudakis,Ram Sundaresh,Alexandra Larsen,William Ruff,Jennifer Schiller,Hugo Guerrero-Cázares,Peter Burger,Roberto Salvatori,Alfredo Quiñones-Hinojosa
View all publications
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組合せ製品

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Primary Antibodies

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSH beta antibody [EPR8198] (AB155958)

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Primary Antibodies

AB131170

Anti-hCG beta antibody [EPHCGR2]

20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hCG beta antibody [EPHCGR2] (AB131170)

  • 0
  • 0

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