Anti-CFTR 抗体 (ab181782)
Key features and details
- Rabbit polyclonal to CFTR
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-CFTR antibody
CFTR 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to CFTR -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse
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免疫原
Synthetic peptide corresponding to Human CFTR.
Database link: P13569 -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
Constituents: 1.21% Tris, 0.75% Glycine, 2% Sucrose -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab181782の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/1000. Predicted molecular weight: 168 kDa.
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| ICC/IF |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
|
WB
1/500 - 1/1000. Predicted molecular weight: 168 kDa. |
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ICC/IF
Use at an assay dependent concentration. |
ターゲット情報
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機能
Involved in the transport of chloride ions. May regulate bicarbonate secretion and salvage in epithelial cells by regulating the SLC4A7 transporter. -
組織特異性
Found on the surface of the epithelial cells that line the lungs and other organs. -
関連疾患
Defects in CFTR are the cause of cystic fibrosis (CF) [MIM:219700]; also known as mucoviscidosis. CF is the most common genetic disease in the Caucasian population, with a prevalence of about 1 in 2'000 live births. Inheritance is autosomal recessive. CF is a common generalized disorder of exocrine gland function which impairs clearance of secretions in a variety of organs. It is characterized by the triad of chronic bronchopulmonary disease (with recurrent respiratory infections), pancreatic insufficiency (which leads to malabsorption and growth retardation) and elevated sweat electrolytes.
Defects in CFTR are the cause of congenital bilateral absence of the vas deferens (CBAVD) [MIM:277180]. CBAVD is an important cause of sterility in men and could represent an incomplete form of cystic fibrosis, as the majority of men suffering from cystic fibrosis lack the vas deferens. -
配列類似性
Belongs to the ABC transporter superfamily. ABCC family. CFTR transporter (TC 3.A.1.202) subfamily.
Contains 2 ABC transmembrane type-1 domains.
Contains 2 ABC transporter domains. -
ドメイン
The PDZ-binding motif mediates interactions with GOPC and with the SLC4A7, SLC9A3R1/EBP50 complex. -
翻訳後修飾
Phosphorylated; activates the channel. It is not clear whether PKC phosphorylation itself activates the channel or permits activation by phosphorylation at PKA sites.
Ubiquitinated, leading to its degradation in the lysosome. Deubiquitination by USP10 in early endosomes, enhances its endocytic recycling. -
細胞内局在
Early endosome membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 1080 Human
- Entrez Gene: 12638 Mouse
- Omim: 602421 Human
- SwissProt: P13569 Human
- SwissProt: P26361 Mouse
- Unigene: 489786 Human
- Unigene: 621460 Human
- Unigene: 661104 Human
see all -
別名
- ABC 35 antibody
- ABC35 antibody
- ABCC 7 antibody
see all
画像
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Western blot - Anti-CFTR antibody (ab181782)All lanes : Anti-CFTR antibody (ab181782) at 1/500 dilution
Lane 1 : BHK cells
Lane 2 : BHK cells transfected with CFTR-3HA tag
Lane 3 : Immunprecipitated wt CFTR from BHK cells
Predicted band size: 168 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-CFTR antibody (ab181782)Immunofluorescent analysis of BHK cells expressing wild-type (wt) or mutated (rΔF508) CFTR, labeling CFTR with ab181782.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (7)
ab181782 は 7 報の論文で使用されています。
- Chen Y et al. Deletion of sphingosine kinase 2 attenuates cigarette smoke-mediated chronic obstructive pulmonary disease-like symptoms by reducing lung inflammation. Biomol Biomed 23:259-270 (2023). PubMed: 36226596
- Cao T et al. A Hyperglycemic Microenvironment Inhibits Tendon-to-Bone Healing through the let-7b-5p/CFTR Pathway. Comput Math Methods Med 2022:8268067 (2022). PubMed: 35126637
- Zhan Y et al. Effects of Maren Pills on the Intestinal Microflora and Short-Chain Fatty Acid Profile in Drug-Induced Slow Transit Constipation Model Rats. Front Pharmacol 13:804723 (2022). PubMed: 35496291
- Hu J et al. LncRNA MITA1 promotes gefitinib resistance by inducing autophagy in lung cancer cells. Biochem Biophys Res Commun 551:21-26 (2021). PubMed: 33714755
- McCarron A et al. Phenotypic Characterization and Comparison of Cystic Fibrosis Rat Models Generated Using CRISPR/Cas9 Gene Editing. Am J Pathol 190:977-993 (2020). PubMed: 32084371
- Xu X et al. Effect of lentivirus-mediated CFTR overexpression on oxidative stress injury and inflammatory response in the lung tissue of COPD mouse model. Biosci Rep 40:N/A (2020). PubMed: 31894837
- Sasaki S et al. Steric Inhibition of 5' UTR Regulatory Elements Results in Upregulation of Human CFTR. Mol Ther 27:1749-1757 (2019). PubMed: 31351782