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リコンビナントRabMAb

Anti-CFHR3 抗体 [EPR25759-72] (ab302792)

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Western blot - Anti-CFHR3 antibody [EPR25759-72] (AB302792)
  • Western blot - Anti-CFHR3 antibody [EPR25759-72] (AB302792)
  • Western blot - Anti-CFHR3 antibody [EPR25759-72] (ab302792)
  • Immunocytochemistry/ Immunofluorescence - Anti-CFHR3 antibody [EPR25759-72] (AB302792)
  • Immunoprecipitation - Anti-CFHR3 antibody [EPR25759-72] (AB302792)
  • Anti-CFHR3 antibody [EPR25759-72] (AB302792)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR25759-72] to Complement factor H-related protein 3
  • Suitable for: WB, IP, ICC/IF
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-CFHR3 antibody [EPR25759-72]
    Complement factor H-related protein 3 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR25759-72] to Complement factor H-related protein 3

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IP, ICC/IFmore details
    適用なし: Flow Cyt (Intra) or IHC-P

  • 種交差性

    交差種: Human
    非交差種: Mouse, Rat

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: Whole cell lysates: Daudi (human Burkitt's lymphoma lymphoblast), Raji (human Burkitt's lymphoma B lymphocyte), Low expression: HepG2 (human hepatocellular carcinoma epithelial cell), untreated human plasma, hman plasma treated with Protein Deglycosylation Mix II, His-tagged human CFHR3 recombinant protein. ICC/IF: Raji (human Burkitt's lymphoma B lymphocyte). IP: Raji (human Burkitt's lymphoma B lymphocyte).

  • 特記事項

    ab302792 does not react in: WB with mouse and rat samples; ICC/IF and IP with mouse tissues; intracellular flow cytometry with human and mouse specimens. 

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR25759-72

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab302792の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/1000. Predicted molecular weight: 37 kDa.

Observed Mass of target (kDa) 40, 35,50, 37, 35,45-50
IP
1/30.
ICC/IF
1/50.
特記事項
WB
1/1000. Predicted molecular weight: 37 kDa.

Observed Mass of target (kDa) 40, 35,50, 37, 35,45-50
IP
1/30.
ICC/IF
1/50.
追加情報
Is unsuitable for Flow Cyt (Intra) or IHC-P.

ターゲット情報

  • 機能

    Might be involved in complement regulation.

  • 組織特異性

    Expressed by the liver and secreted in plasma.

  • 配列類似性

    Contains 5 Sushi (CCP/SCR) domains.

  • 細胞内局在

    Secreted.
  • Information by UniProt

  • 参照データベース

    • 別名

      • CFHR3 antibody
      • Complement factor H-related protein 3 antibody
      • DOWN16 antibody
      • FHR-3 antibody
      • FHR3_HUMAN antibody
      • H factor-like protein 3 antibody
      see all

    画像

    • Western blot - Anti-CFHR3 antibody [EPR25759-72] (AB302792)
      Western blot - Anti-CFHR3 antibody [EPR25759-72] (AB302792)
      All lanes : Anti-CFHR3 antibody [EPR25759-72] (ab302792) at 1/1000 dilution

      Lane 1 : His-tagged human CFHR3 recombinant protein 10 ng
      Lane 2 : His-tagged human CFHR4 recombinant protein 10 ng

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 37 kDa
      Observed band size: 45-50 kDa why is the actual band size different from the predicted?


      Exposure time: 10 seconds


      Blocking and dilution buffer and concentration: 5% NFDM/TBST. 

      This antibody does not cross-react with human CFHR4.

      Both recombinant proteins were made in-house and expressed from a mammalian expression system.

    • Western blot - Anti-CFHR3 antibody [EPR25759-72] (AB302792)
      Western blot - Anti-CFHR3 antibody [EPR25759-72] (AB302792)
      All lanes : Anti-CFHR3 antibody [EPR25759-72] (ab302792) at 1/1000 dilution

      Lane 1 : Untreated human plasma
      Lane 2 : Human plasma treated with Protein Deglycosylation Mix II

      Lysates/proteins at 15 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

      Predicted band size: 37 kDa
      Observed band size: 50, 37, 35 kDa why is the actual band size different from the predicted?


      Exposure time: 103 seconds


      Blocking and dilution buffer and concentration: 5% NFDM/TBST. 

      CFHR3 is a glycoprotein of approximately 50 kDa and detected as 37/35 kDa bands after Protein Deglycosylation Mix II treatment.(PMID: 23830046)

    • Western blot - Anti-CFHR3 antibody [EPR25759-72] (ab302792)
      Western blot - Anti-CFHR3 antibody [EPR25759-72] (ab302792)
      All lanes : Anti-CFHR3 antibody [EPR25759-72] (ab302792) at 1/1000 dilution

      Lane 1 : Daudi (human Burkitt's lymphoma lymphoblast), whole cell lysate
      Lane 2 : Raji (human Burkitt's lymphoma B lymphocyte), whole cell lysate
      Lane 3 : HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Developed using the ECL technique.

      Predicted band size: 37 kDa
      Observed band size: 40, 35 kDa why is the actual band size different from the predicted?


      Exposure time: 37 seconds


      Blocking and dilution buffer and concentration: 5% NFDM/TBST

      The doublet bands represent two isoforms of CFHR3 (PMID: 8428964).
       
      Low expression: HepG2 (Human Protein Atlas).
       
      This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.
    • Immunocytochemistry/ Immunofluorescence - Anti-CFHR3 antibody [EPR25759-72] (AB302792)
      Immunocytochemistry/ Immunofluorescence - Anti-CFHR3 antibody [EPR25759-72] (AB302792)

      Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Raji (human Burkitt's lymphoma B lymphocyte) cells labeling CFHR3 with ab302792 at 1/50 dilution (10.74 µg/ml), followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (2 µg/ml) (Green). Confocal image showing cytoplasmic staining in Raji cell line and weak staining in HepG2 cell line. Low expression is observed in HepG2. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (2.5 µg/ml) (Red). The Nuclear counterstain was DAPI (Blue).

      Secondary antibody only control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 µg/ml).

    • Immunoprecipitation - Anti-CFHR3 antibody [EPR25759-72] (AB302792)
      Immunoprecipitation - Anti-CFHR3 antibody [EPR25759-72] (AB302792)

      CFHR3 was immunoprecipitated from 0.35 mg Raji (human Burkitt's lymphoma B lymphocyte), whole cell lysate with ab302792 at 1/30 dilution (2 µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab302792 at 1/1000 dilution. VeriBlot for IP secondary antibody (HRP)(ab131366) was used at 1/5000 dilution.

      Lane 1: Raji (human Burkitt's lymphoma B lymphocyte), whole cell lysate 10 µg (Inset)

      Lane 2: ab302792 IP in Raji whole cell lysate

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab302792 in Raji whole cell lysate

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 10 seconds

      Obeserved MW (kDa): 35. 

       

    • Anti-CFHR3 antibody [EPR25759-72] (AB302792)
      Anti-CFHR3 antibody [EPR25759-72] (AB302792)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab302792 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab302792 は論文での使用が確認できていません。

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