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AB123626

CF405M Anti-CD45 抗体 [D3/9]

CF405M Anti-CD45 antibody [D3/9]

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(2 Publications)

Mouse Monoclonal CD45 antibody - conjugated to CF®405M. Suitable for Flow Cyt and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Cell preparation containing PTPRC protein.

別名を表示する

CD45, PTPRC, Receptor-type tyrosine-protein phosphatase C, Leukocyte common antigen, T200, L-CA

1 Images
Flow Cytometry - CF405M Anti-CD45 antibody [D3/9] (AB123626)
  • Flow Cyt

Unknown

Flow Cytometry - CF405M Anti-CD45 antibody [D3/9] (AB123626)

ab123626, at 5 μl/106 cells, staining CD45 in normal Human peripheral blood cells by Flow Cytometry.

Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

D3/9

アイソタイプ

IgG1

標識

CF®405M

励起波長/蛍光波長

Ex: 408nm, Em: 452nm

キャリアフリー

No

交差種

Human

アプリケーション

Flow Cyt

applications

免疫原

Cell preparation containing PTPRC protein. The exact immunogen used to generate this antibody is proprietary information.

P08575

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "5 µL for 10^6 Cells", "FlowCyt-species-notes": "<p><a href='/products/primary-antibodies/cf405m-mouse-igg1-kappa-monoclonal-b11-6-isotype-control-ab126026'>ab126026</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>" } } }
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製品の詳細

CF405M (Abs/Em Max: 408/450nm). Direct replacement for Pacific Blue dye®, BD Horizon™ V450.
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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Immunogen
バッファー組成
pH: 7.2 Preservative: 0.09% Sodium azide
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
+4°C
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

CD45 also known as Protein tyrosine phosphatase receptor type C (PTPRC) is a transmembrane glycoprotein with a molecular mass ranging between 180-240 kDa depending on its isoform. It is expressed on the surface of almost all hematopoietic cells except for mature erythrocytes and platelets. CD45 has a critical role in regulating antigen receptor signaling by modifying kinases involved in signal transduction and it is essential in lymphocyte development and activation. Because of its broad expression on immune cells CD45 is a valuable marker for differentiating various immune cell types in assays like flow cytometry and immunohistochemistry (IHC) often referred to as CD45 stain.
Biological function summary

CD45 acts by regulating tyrosine phosphorylation in the immune cell signaling context participating directly in signal transduction. It functions by dephosphorylating specific phosphotyrosine residues on various proteins modulating the signaling threshold required for lymphocyte activation. Although not known to be part of a protein complex CD45 itself shows isoform variation that associates with specific immune cell types impacting their function. Importantly CD45 interacts with multiple signaling molecules to affect cell growth and differentiation.

Pathways

Scientists associate CD45 with signaling pathways such as the T-cell receptor (TCR) and B-cell receptor (BCR) signaling. CD45 modulates the activity of Src family kinases important elements in these pathways making it essential for effective immune response and tolerance. Furthermore interactions between CD45 and proteins like Lck in T-cells or Lyn in B-cells highlight its pivotal role in executing its signaling functions.

Scientists often link CD45 to immune-related conditions including autoimmunity and leukemia. In autoimmune diseases altered CD45 expression or activity can disrupt normal immune function contributing to pathogenesis. In leukemia CD45 expression levels can assist in disease classification and prognosis as it interacts with proteins involved in cell cycle regulation. The anti-CD45 antibodies can provide diagnostic and therapeutic avenues highlighting their utility in disease management.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor (PubMed : 35767951). Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity (By similarity).. (Microbial infection) Acts as a receptor for human cytomegalovirus protein UL11 and mediates binding of UL11 to T-cells, leading to reduced induction of tyrosine phosphorylation of multiple signaling proteins upon T-cell receptor stimulation and impaired T-cell proliferation.
See full target information PTPRC
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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Macromolecular bioscience 24:e2300270 PubMed37700543

2023

Crosslinked Collagen-Hyaluronic Acid Scaffold Enhances Interleukin-10 Under Co-Culture of Macrophages And Adipose-Derived Stem Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Moema de Alencar Hausen,Ariana de Souza Moraes,Flavia Pedrini,Felipe Grabarz,José Angelo Camilli,Eliana Aparecida de Rezende Duek

Nanoscale advances 5:4435-4446 PubMed37638157

2023

Separation and isolation of CD9-positive extracellular vesicles from plasma using flow cytometry.

Applications

Unspecified application

Species

Unspecified reactive species

Karan Khanna,Nikki Salmond,Sina Halvaei,Andrew Johnson,Karla C Williams
View all publications
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代替製品

Primary Antibodies

AB200315

Alexa Fluor® 488 Anti-CD45 antibody [EP322Y]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-CD45 antibody [EP322Y] (AB200315)

  • 0
  • 0
Primary Antibodies

AB221245

APC Anti-CD45 antibody [EP322Y]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - APC Anti-CD45 antibody [EP322Y] (AB221245)

  • 0
  • 0
Primary Antibodies

AB40763

Anti-CD45 antibody [EP322Y]

20ul selling size|RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-CD45 antibody [EP322Y] (AB40763)

  • 5
  • 14
Primary Antibodies

AB208022

Anti-CD45 antibody [EPR20033]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD45 antibody [EPR20033] (AB208022)

  • 5
  • 2
Primary Antibodies

AB229292

Anti-CD45 antibody [EPR20033] - BSA and Azide free

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD45 antibody [EPR20033] - BSA and Azide free (AB229292)

  • 5
  • 2
Primary Antibodies

AB214437

Anti-CD45 antibody [EP322Y] - BSA and Azide free

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-CD45 antibody [EP322Y] - BSA and Azide free (AB214437)

  • 5
  • 1

組合せ製品

Assay Kits

AB277445

Mouse CD45 ELISA kit

ELISA - Mouse CD45 ELISA kit (AB277445)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB223930

PE Anti-CD34 antibody [EP373Y]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - PE Anti-CD34 antibody [EP373Y] (AB223930)

  • 5
  • 1
Primary Antibodies

AB314277

PE Anti-CD105 antibody [EPR22811-18]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - PE Anti-CD105 antibody [EPR22811-18] (AB314277)

  • 0
  • 0
Primary Antibodies

AB282789

PE Anti-CD73 antibody [EPR23048-139]

RabMAb|Recombinant

Flow Cytometry - PE Anti-CD73 antibody [EPR23048-139] (AB282789)

  • 0
  • 0

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