Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
リコンビナントRabMAb

Anti-CES2 抗体 [EPR14856] (ab184957)

Reviews (1) Submit a question References (3)
Western blot - Anti-CES2 antibody [EPR14856] (ab184957)
  • Western blot - Anti-CES2 antibody [EPR14856] (ab184957)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CES2 antibody [EPR14856] (ab184957)
  • Anti-CES2 antibody [EPR14856] (ab184957)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14856] to CES2
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-CES2 antibody [EPR14856]
    CES2 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR14856] to CES2

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IHC-Pmore details

  • 種交差性

    交差種: Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • HepG2 cell lysate, Human fetal liver lysate, Human colon tissue

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab184957の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB (1)
1/1000 - 1/2000. Detects a band of approximately 62 kDa (predicted molecular weight: 62 kDa).
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
特記事項
WB
1/1000 - 1/2000. Detects a band of approximately 62 kDa (predicted molecular weight: 62 kDa).
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

ターゲット情報

  • 機能

    Involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs. Shows high catalytic efficiency for hydrolysis of cocaine, 4-methyumbelliferyl acetate, heroin and 6-monoacetylmorphine.

  • 組織特異性

    Preferentially expressed in intestine with moderate expression in liver. Within the intestine, highest expression is found in small intestine with lower expression in colon and rectum.

  • 配列類似性

    Belongs to the type-B carboxylesterase/lipase family.

  • 翻訳後修飾

    Glycosylated.

  • 細胞内局在

    Endoplasmic reticulum lumen.
  • Information by UniProt

  • 参照データベース

    • 別名

      • Carboxylesterase 2 (intestine, liver) antibody
      • Carboxylesterase 2 antibody
      • CE-2 antibody
      • CES2 antibody
      • CES2A1 antibody
      • Cocaine esterase antibody
      • EC 3.1.1.1 antibody
      • EST2_HUMAN antibody
      • hCE-2 antibody
      • ICE antibody
      • Iintestinal carboxylesterase liver carboxylesterase 2 antibody
      • Intestinal carboxylesterase; liver carboxylesterase-2 antibody
      • Methylumbelliferyl acetate deacetylase 2 antibody
      • PCE 2 antibody
      • PCE-2 antibody
      see all

    画像

    • Western blot - Anti-CES2 antibody [EPR14856] (ab184957)
      Western blot - Anti-CES2 antibody [EPR14856] (ab184957)
      Anti-CES2 antibody [EPR14856] (ab184957) at 1/1000 dilution + HepG2 cell lysate at 20 µg

      Secondary
      Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 62 kDa

    • Western blot - Anti-CES2 antibody [EPR14856] (ab184957)
      Western blot - Anti-CES2 antibody [EPR14856] (ab184957)
      Anti-CES2 antibody [EPR14856] (ab184957) at 1/1000 dilution + Human fetal liver lysate at 10 µg

      Secondary
      Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 62 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CES2 antibody [EPR14856] (ab184957)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CES2 antibody [EPR14856] (ab184957)

      Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling CES2 with ab184957 at 1/500 dilution.  Counterstained with Hematoxylin.

      Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

    • Anti-CES2 antibody [EPR14856] (ab184957)
      Anti-CES2 antibody [EPR14856] (ab184957)

    参考文献 (3)

    ab184957 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab184957 は 3 報の論文で使用されています。

    • Qin M  et al. Phosphorylation-Induced Ubiquitination and Degradation of PXR through CDK2-TRIM21 Axis. Cells 11:N/A (2022). PubMed: 35053380
    • Li H  et al. Cross-species single-cell transcriptomic analysis reveals divergence of cell composition and functions in mammalian ileum epithelium. Cell Regen 11:19 (2022). PubMed: 35511361
    • Yu J  et al. High-throughput proteomics integrated with gene microarray for discovery of colorectal cancer potential biomarkers. Oncotarget 7:75279-75292 (2016). WB, IHC-P ; Human . PubMed: 27661117

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    Western blot abreview for Anti-CES2 antibody [EPR14856]

     Poor
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (liver)
    Gel Running Conditions
    Reduced Denaturing (8%)
    Loading amount
    30 µg
    Specification
    liver
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Oct 13 2015

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com