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Anti-Ceruloplasmin 抗体 (ab48614)

Reviews (2)Q&A (3)References (15)
Western blot - Anti-Ceruloplasmin antibody (ab48614)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ceruloplasmin antibody (ab48614)

Key features and details

  • Rabbit polyclonal to Ceruloplasmin
  • Suitable for: IHC-P, IP, RIA, EIA, ELISA, ICC/IF, WB, IHC-FoFr
  • Reacts with: Human
  • Isotype: IgG

リコンビナント抗体で、ロット間での高い再現性を実現

Product image
Anti-Ceruloplasmin antibody [EPSISR6-42] (ab157452)
  • 異なるロット間での安定した再現性
  • 容易なスケールアップ
  • 評価試験による特異性の確認済み
  • 倫理基準に準拠 - アニマル・フリーの生産

製品の概要

  • 製品名

    Anti-Ceruloplasmin antibody
    Ceruloplasmin 一次抗体 製品一覧

  • 製品の詳細

    Rabbit polyclonal to Ceruloplasmin

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: IHC-P, IP, RIA, EIA, ELISA, ICC/IF, WB, IHC-FoFrmore details

  • 種交差性

    交差種: Human

  • 免疫原

    Human ceruloplasmin purified from human plasma

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

  • バッファー

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 49.98% PBS, 50% Glycerol (glycerin, glycerine)
  • Concentration information loading...

  • 精製度

    Protein G purified

  • ポリ/モノ

    ポリクローナル

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab48614の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-P
Use a concentration of 2 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IP
Use at an assay dependent concentration.
RIA
Use at an assay dependent concentration.
EIA
Use at an assay dependent concentration.
ELISA
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
WB (1)
Use at an assay dependent concentration. Predicted molecular weight: 122 kDa.
IHC-FoFr (1)
Use at an assay dependent concentration.
特記事項
IHC-P
Use a concentration of 2 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IP
Use at an assay dependent concentration.
RIA
Use at an assay dependent concentration.
EIA
Use at an assay dependent concentration.
ELISA
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 122 kDa.
IHC-FoFr
Use at an assay dependent concentration.

ターゲット情報

  • 機能

    Ceruloplasmin is a blue, copper-binding (6-7 atoms per molecule) glycoprotein. It has ferroxidase activity oxidizing Fe(2+) to Fe(3+) without releasing radical oxygen species. It is involved in iron transport across the cell membrane.

  • 組織特異性

    Expressed by the liver and secreted in plasma.

  • 関連疾患

    Defects in CP are the cause of aceruloplasminemia (ACERULOP) [MIM:604290]. It is an autosomal recessive disorder of iron metabolism characterized by iron accumulation in the brain as well as visceral organs. Clinical features consist of the triad of retinal degeneration, diabetes mellitus and neurological disturbances.
    Note=Ceruloplasmin levels are decreased in Wilson disease, in which copper cannot be incorporated into ceruloplasmin in liver because of defects in the copper-transporting ATPase 2.

  • 配列類似性

    Belongs to the multicopper oxidase family.
    Contains 3 F5/8 type A domains.
    Contains 6 plastocyanin-like domains.

  • 細胞内局在

    Secreted.
  • Information by UniProt

  • 参照データベース

    • 別名

      • CERU_HUMAN antibody
      • Ceruloplasmin antibody
      • CP 2 antibody
      • CP antibody
      • CP2 antibody
      • Ferroxidase antibody
      see all

    画像

    • Western blot - Anti-Ceruloplasmin antibody (ab48614)
      Western blot - Anti-Ceruloplasmin antibody (ab48614)
      Anti-Ceruloplasmin antibody (ab48614) at 1 µg/ml + Human Plasma Total Protein Lysate at 10 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 122 kDa
      Observed band size: 122,148 kDa why is the actual band size different from the predicted?
      Additional bands at: 34 kDa, 76 kDa. We are unsure as to the identity of these extra bands.


      Exposure time: 30 seconds


      Ceruloplasmin contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted (148 kDa).
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ceruloplasmin antibody (ab48614)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ceruloplasmin antibody (ab48614)
      Ab48614 staining human tonsil. Staining is localized to the cytoplasm.
      Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.
      Sections were stained using an automated system (Dako PT Link), at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 antigen retrieval buffer, EDTA pH 9.0. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

    参考文献 (15)

    ab48614 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab48614 は 15 報の論文で使用されています。

    • Ahmad A  et al. Induction of Hepcidin Expression in the Renal Cortex of Sickle Cell Disease Mice. Int J Mol Sci 24:N/A (2023). PubMed: 37445980
    • Jacquemin G  et al. Paracrine signalling between intestinal epithelial and tumour cells induces a regenerative programme. Elife 11:N/A (2022). PubMed: 35543624
    • Su C  et al. Single-Cell RNA Sequencing in Multiple Pathologic Types of Renal Cell Carcinoma Revealed Novel Potential Tumor-Specific Markers. Front Oncol 11:719564 (2021). PubMed: 34722263
    • Shin HJ  et al. Lipocalin-2 Deficiency Reduces Oxidative Stress and Neuroinflammation and Results in Attenuation of Kainic Acid-Induced Hippocampal Cell Death. Antioxidants (Basel) 10:N/A (2021). PubMed: 33445746
    • Madani AY  et al. Signal Transducer and Activator of Transcription 3 (STAT3) Suppresses STAT1/Interferon Signaling Pathway and Inflammation in Senescent Preadipocytes. Antioxidants (Basel) 10:N/A (2021). PubMed: 33672392
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-5 of 5 Abreviews or Q&A

    Western blot abreview for Anti-Ceruloplasmin antibody

     Good
    Abreviews
    Application
    Western blot
    Loading amount
    1 µg
    Gel Running Conditions
    Reduced Denaturing (10% PAGE)
    Sample
    Fish Serum (plasma)
    Specification
    plasma
    Blocking step
    (agent) for 24 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Jan 26 2015

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Ceruloplasmin antibody

     Excellent
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Mouse Cell (Duodenum)
    Specification
    Duodenum
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.3% TritonX in 0.1% PBS
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Ruma Raha-Chowdhury

    Verified customer

    投稿 Sep 02 2011

    Question

    For the below antibodies I am looking for both isotype control and positive controls. It would be great if you could give the information for both in case you have them.
    ab36204 --> Rabbit IgG
    ab48614 --> Rabbit IgG
    ab66219 --> Rabbit IgG
    ab83508 --> Mouse IgG1
    ab102998 --> Rabbit IgG
    In the list I was asking for isotype controls, but still the positive controls would be interesting to know, too.
    All the best,

    Read More
    Answer

    Thank you for your clarification.

    I can confirm the concentration of ab66219 (Batch: GR9812) of this ab is 1 mg/ml. The other antibody ab36204 (Lot: GR42382) is not affinity purified but whole antiserum so the IgG is not quantified. However, I can provide a range of the IgG: 10-15 mg/ml.

    Isotype controls:



    can be searched at this site: https://www.abcam.com/index.html?c=718

    ab36204, ab48614, ab66219, ab102998 --> Rabbit IgG - ab37406, ab125938, ab128142

    ab83508 --> Mouse IgG1 - ab91353, ab27479, ab81032



    Positive control:



    ab36204 - CRMP4 - It is mainly expressed in heart and skeletal muscle and also strongly expressed in fetal brain and spinal cord (human, mouse or rat),

    ab48614 --Ceruloplasmin - It is expressed by the liver and secreted in plasma,

    ab66219 -- Oprl1 - Brain (cortex),

    ab83508 -- NG2 - It is detected only in malignant melanoma cells, SK-MEL-28 cells,

    ab102998 --B3GNT5- only tested in WB - Widely expressed. It is highly expressed in lung, colon, placenta, testis, pituitary gland and cerebellum, MDA-MB435 cell line

    If you need any further assistance in the future, please do not hesitate to contact me.

    Read More

    Question

    Dear Sir or Madam,
    We recently bought the following antibodies from your company: ab36204; ab48614; ab66219; ab83508; ab102998. As we would like to test at least four of them on 4%PFA, sucrose fixed brain tissue and include the correct controls I have a few questions:
    I received the following list:
    ab36204 (Lot: GR42382) --> concentration: N/A 100µl
    ab48614 (Lot: GR62244) --> concentration: 0,7699 mg/ml (100µg)
    ab66219 (Lot: GR9812) --> concentration: N/A 100µl
    ab83508 (Lot: GR38090) --> concentration: 0.5 mg/ml (100µg)
    ab102998 (Lot: GR80395) --> concentration 0.25mg/ml (100µg)
    In order to include a control with the same antibody concentration as for the primary antibody it would be good to know the concentration of ab36204 and ab66219, too. Could you help me concerning that matter?
    Second question, do you have any experience with good control antibodies? I thought the following:
    ab36204 --> Rabbit IgG
    ab48614 --> Rabbit IgG
    ab66219 --> Rabbit IgG
    ab83508 --> Mouse IgG1
    ab102998 --> Rabbit IgG
    I think that is also what you suggested in the data sheets, I just would like to make sure, that I am correct.
    I would be very happy about a rapid answer.
    Best regards,

    Read More
    Answer

    Thank you for your enquiry and your interest in our products.

    This is to confirm that we can only provide the concentration for affinity purified antibodies. For whole serum or supernatant we do not quantify the IgG concentration. I have contacted the lab to get the requested information for you concerning ab66219 (Lot: GR9812).

    Would you be so kind to clarify if you wish to look for isotype control or positive control for these primary antibodies listed below?

    ab36204 --> Rabbit IgG
    ab48614 --> Rabbit IgG
    ab66219 --> Rabbit IgG
    ab83508 --> Mouse IgG1
    ab102998 --> Rabbit IgG

    I look forward to hearing from you soon. As soon as I have further information on the concentration (ab66219), I will contact you again.

    Read More

    Question

    ¿Podríais por favor informarme si los anticuerpos ab110449 y ab48614 pueden conjugarse?
    Nos gustaría conjugarlos con HRP o fosfatasa alcalina.
    Muchas gracias.

    Read More
    Answer

    Gracias po contactarnos.

    En principio no habría ningún problema para conjugar estos anticuerpos. Lo que si es necesario tener en cuenta es que el kit de conjugación sea compatible con los buffers y aditivos en los que conserva el anticuerpo.
    Ignoro si disponéis de un kit de conjugación de anticuerpos, si os hiciera falta existen en nuestro catálogo los kits EasyLink para llevar a cabo la conjugación de anticuerpos con distintas enzimas o fluorocromos.
    https://www.abcam.com/index.html?pageconfig=resource&rid=13148]
    En el apartado correspondiente a las cuestiones técnicas del uso de estos kits encontrarás los buffers compatibles con los reactivos del kit, así como cuestiones relativas a la purificación y la concentración del anticuerpo que resultan útiles antes de usarlos.
    https://www.abcam.com/index.html?pageconfig=resource&rid=13156
    En el primero de los links indicado hay a su vez links directos a los kits de conjugación EasyLink tanto con HRP como con AP.
    Espero que esta información te sea útil, de lo contrario, por favor no dudes en volver a contactarnos.

    Read More

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