Anti-CERK 抗体 (ab155061)
Key features and details
- Rabbit polyclonal to CERK
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-CERK antibody -
製品の詳細
Rabbit polyclonal to CERK -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat
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免疫原
Recombinant fragment, corresponding to a region within amino acids 85-335 of Human CERK (Uniprot ID: Q8TCT0).
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ポジティブ･コントロール
- Human breast carcinoma tissue; H1299, HeLa, HepG2 and Raji whole cell lysates.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab155061の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 60 kDa.
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| IHC-P |
1/100 - 1/1000.
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| 特記事項 |
|---|
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WB
1/500 - 1/3000. Predicted molecular weight: 60 kDa. |
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IHC-P
1/100 - 1/1000. |
ターゲット情報
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機能
Catalyzes specifically the phosphorylation of ceramide to form ceramide 1-phosphate. Acts efficiently on natural and analog ceramides (C6, C8, C16 ceramides, and C8-dihydroceramide), to a lesser extent on C2-ceramide and C6-dihydroceramide, but not on other lipids, such as various sphingosines. Binds phosphoinositides. -
組織特異性
High level expression in heart, brain, skeletal muscle, kidney and liver; moderate in peripheral blood leukocytes and thymus; very low in spleen, small intestine, placenta and lung. -
配列類似性
Contains 1 DAGKc domain. -
細胞内局在
Cytoplasm. Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 64781 Human
- Entrez Gene: 223753 Mouse
- Entrez Gene: 300129 Rat
- Omim: 610307 Human
- SwissProt: Q8TCT0 Human
- SwissProt: Q8K4Q7 Mouse
- Unigene: 200668 Human
- Unigene: 222685 Mouse
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別名
- Acylsphingosine kinase antibody
- Ceramide kinase antibody
- Cerk antibody
see all
画像
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Western blot - Anti-CERK antibody (ab155061)Anti-CERK antibody (ab155061) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg
Predicted band size: 60 kDa
7.5% SDS-PAGE. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CERK antibody (ab155061)Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling CERK with ab155061 at 1/500 dilution.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab155061 は 1 報の論文で使用されています。
- Ku CW et al. Decreased Innate Migration of Pro-Inflammatory M1 Macrophages through the Mesothelial Membrane Is Affected by Ceramide Kinase and Ceramide 1-P. Int J Mol Sci 23:N/A (2022). PubMed: 36555618