Anti-Centaurin alpha 1 抗体 (ab27476)
Key features and details
- Goat polyclonal to Centaurin alpha 1
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human, Pig
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
-
製品名
Anti-Centaurin alpha 1 antibody
Centaurin alpha 1 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to Centaurin alpha 1 -
由来種
Goat -
アプリケーション
適用あり: IHC-P, WBmore details -
種交差性
交差種: Mouse, Rat, Human, Pig -
免疫原
Synthetic peptide:
CQEYAVEAHFKHKP
, corresponding to amino acids 362-374 of Human Centaurin alpha 1. -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% Tris, 0.5% BSA -
Concentration information loading...
-
精製度
Immunogen affinity purified -
特記事項(精製)
This antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab27476の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
IHC-P |
Use at an assay dependent concentration.
|
|
WB |
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 43 kDa).
1 hour primary incubation is recommended for this product. |
特記事項 |
---|
IHC-P
Use at an assay dependent concentration. |
WB
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 43 kDa). 1 hour primary incubation is recommended for this product. |
ターゲット情報
-
関連性
The centaurin alpha 1 protein is a high affinity PtdIns(3,4,5)P3 binding protein enriched in brain. By acting as a GTPase activating protein for ADP ribosylation factor 6 (ARF6), centaurin alpha 1 is able to switch off ARF6 and inhibit its ability to mediate beta 2 adrenoceptor internalization and negatively regulates ARF6 activity by functioning as an in vivo PIP3 dependent ARF6 GAP. -
細胞内局在
Cytoplasmic, Nuclear and Plasma membrane -
参照データベース
- Entrez Gene: 11033 Human
- Entrez Gene: 231821 Mouse
- Entrez Gene: 397453 Pig
- Entrez Gene: 171097 Rat
- Omim: 608114 Human
- SwissProt: O75689 Human
-
別名
- ArfGAP with dual PH domains 1 antibody
- CENTA 1 antibody
- CENTA1 antibody
see all
画像
-
All lanes : Anti-Centaurin alpha 1 antibody (ab27476) at 1 µg/ml
Lane 1 : Human brain tissue lysate
Lane 2 : Mouse brain tissue lysate
Lane 3 : Rat brain tissue lysate
Lane 4 : Pig brain tissue lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 43 kDaPrimary incubation was 1 hour. Detected by chemiluminescence.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Centaurin alpha 1 antibody (ab27476)IHC image of ab27476 staining in human normal hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab27476, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
プロトコール
データシートおよび資料
-
Datasheet download
参考文献 (4)
ab27476 は 4 報の論文で使用されています。
- Gutiérrez Y et al. KIF13A drives AMPA receptor synaptic delivery for long-term potentiation via endosomal remodeling. J Cell Biol 220:N/A (2021). PubMed: 33999113
- Van Duzer A et al. ADAP1 promotes invasive squamous cell carcinoma progression and predicts patient survival. Life Sci Alliance 2:N/A (2019). PubMed: 31792062
- Szatmari EM et al. Centaurin-a1-Ras-Elk-1 signaling at mitochondria mediates ß-amyloid-induced synaptic dysfunction. J Neurosci 33:5367-74 (2013). WB ; Rat . PubMed: 23516302
- Moore CD et al. The neuronal Arf GAP centaurin alpha1 modulates dendritic differentiation. J Cell Sci 120:2683-93 (2007). WB, ICC/IF, IHC-P ; Human, Rat . PubMed: 17635995