Anti-CENPT 抗体 (ab86595)
Key features and details
- Rabbit polyclonal to CENPT
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-CENPT antibody
CENPT 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to CENPT -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Human
交差が予測される動物種: Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant
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免疫原
Synthetic peptide corresponding to a region within amino acids 480 - 520 (LEMVEKCLDK YFQHLCDDLE VFAAHAGRKT VKPEDLELLM) of Human CENPT (NP_079358.3)
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ポジティブ･コントロール
- Whole cell lysate from HeLa cells or 293T cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab86595の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/2000 - 1/10000. Predicted molecular weight: 60 kDa.
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| IP |
Use at 10 µg/mg of lysate.
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| 特記事項 |
|---|
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WB
1/2000 - 1/10000. Predicted molecular weight: 60 kDa. |
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IP
Use at 10 µg/mg of lysate. |
ターゲット情報
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機能
Component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres. CENP-T is one of the basic inner kinetochore proteins with most further proteins binding downstream, suggesting a fundamental role of CENP-T in kinetochore function. -
配列類似性
Belongs to the CENPT family. -
翻訳後修飾
Phosphorylated upon DNA damage, probably by ATM or ATR. -
細胞内局在
Nucleus. Chromosome > centromere > kinetochore. Localizes exclusively in the kinetochore domain of centromeres. - Information by UniProt
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参照データベース
- Entrez Gene: 80152 Human
- Omim: 611510 Human
- SwissProt: Q96BT3 Human
- Unigene: 288382 Human
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別名
- C16orf56 antibody
- CENP T antibody
- CENP-T antibody
see all
画像
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Western blot - Anti-CENPT antibody (ab86595)All lanes : Anti-CENPT antibody (ab86595) at 0.1 µg/ml
Lane 1 : Whole cell lysate from HeLa cells at 50 µg
Lane 2 : Whole cell lysate from HeLa cells at 15 µg
Lane 3 : Whole cell lysate from HeLa cells at 5 µg
Lane 4 : Whole cell lysate from 293T cells at 50 µg
Predicted band size: 60 kDa
Exposure time: 3 minutes -
Immunoprecipitation - Anti-CENPT antibody (ab86595)
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab86595 は 1 報の論文で使用されています。
- Tarasovetc EV et al. Permitted and restricted steps of human kinetochore assembly in mitotic cell extracts. Mol Biol Cell 32:1241-1255 (2021). PubMed: 33956511