Anti-CENPB 抗体 (ab167361)
Key features and details
- Mouse polyclonal to CENPB
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-CENPB antibody
CENPB 一次抗体 製品一覧 -
製品の詳細
Mouse polyclonal to CENPB -
由来種
Mouse -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Human -
免疫原
Recombinant full length protein within Human CENPB aa 1-600. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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ポジティブ･コントロール
- Recombinant Human CENPB protein (ab73636) can be used as a positive control in WB. A431 cell lysate, CENPB transfected 293T cell line lysate, HeLa cells
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
バッファー
pH: 7.4
Constituent: 99% PBS -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab167361の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 65 kDa.
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| ICC/IF |
Use a concentration of 10 µg/ml.
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| 特記事項 |
|---|
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 65 kDa. |
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ICC/IF
Use a concentration of 10 µg/ml. |
ターゲット情報
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機能
Interacts with centromeric heterochromatin in chromosomes and binds to a specific subset of alphoid satellite DNA, called the CENP-B box. May organize arrays of centromere satellite DNA into a higher order structure which then directs centromere formation and kinetochore assembly in mammalian chromosomes. -
配列類似性
Contains 1 HTH CENPB-type DNA-binding domain.
Contains 1 HTH psq-type DNA-binding domain. -
細胞内局在
Nucleus. Chromosome > centromere. - Information by UniProt
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参照データベース
- Entrez Gene: 1059 Human
- Omim: 117140 Human
- SwissProt: P07199 Human
- Unigene: 516855 Human
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別名
- CENP B antibody
- CENP-B antibody
- CENPB antibody
see all
画像
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Western blot - Anti-CENPB antibody (ab167361)Anti-CENPB antibody (ab167361) at 1 µg/ml + A431 cell lysate at 50 µg
Secondary
Goat anti-Mouse IgG HRP at 1/2500 dilution
Predicted band size: 65 kDa -
Western blot - Anti-CENPB antibody (ab167361)All lanes : Anti-CENPB antibody (ab167361) at 1 µg/ml
Lane 1 : CENPB transfected 293T cell line lysate
Lane 2 : Non-transfected 293T cell line lysate
Lysates/proteins at 15 µl per lane.
Secondary
All lanes : Goat anti-Mouse IgG HRP at 1/2500 dilution
Predicted band size: 65 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-CENPB antibody (ab167361)Immunofluorescence analysis of HeLa cells labeling CENPB using ab167361 at 10 µg/ml.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (2)
ab167361 は 2 報の論文で使用されています。
- Murillo-Pineda M et al. Induction of spontaneous human neocentromere formation and long-term maturation. J Cell Biol 220:N/A (2021). PubMed: 33443568
- Mitra S et al. Genetic screening identifies a SUMO protease dynamically maintaining centromeric chromatin. Nat Commun 11:501 (2020). PubMed: 31980633
