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Anti-Cenexin1/ODF2 抗体 (ab43840)

Reviews (4)Q&A (5)References (36)
Western blot - Anti-Cenexin1/ODF2 antibody (ab43840)
  • Immunocytochemistry/ Immunofluorescence - Anti-Cenexin1/ODF2 antibody (ab43840)
  • Western blot - Anti-Cenexin1/ODF2 antibody (ab43840)

Key features and details

  • Rabbit polyclonal to Cenexin1/ODF2
  • Suitable for: WB, ICC/IF
  • Reacts with: Mouse, Human
  • Isotype: IgG

リコンビナント抗体で、ロット間での高い再現性を実現

Product image
Anti-Cenexin1/ODF2 antibody [EPR13561] (ab186412)
  • 異なるロット間での安定した再現性
  • 容易なスケールアップ
  • 評価試験による特異性の確認済み
  • 倫理基準に準拠 - アニマル・フリーの生産

製品の概要

  • 製品名

    Anti-Cenexin1/ODF2 antibody
    Cenexin1/ODF2 一次抗体 製品一覧

  • 製品の詳細

    Rabbit polyclonal to Cenexin1/ODF2

  • 由来種

    Rabbit

  • 特異性

    On the basis of the immunogen sequence, we would expect this antibody to recognise the following isoforms of Cenexin1/ODF2 as listed in Swissprot: Q5BJF6-1 (95 kDa), Q5BJF6-2 (89 kDa), Q5BJF6-3 (93 kDa), and Q5BJF6-4 (95 kDa).

  • アプリケーション

    適用あり: WB, ICC/IFmore details

  • 種交差性

    交差種: Mouse, Human

  • 免疫原

    Synthetic peptide corresponding to Human Cenexin1/ODF2 aa 800 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab43839)

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 98.98% PBS, 1% BSA

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...

  • 精製度

    Immunogen affinity purified

  • ポリ/モノ

    ポリクローナル

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab43840の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB (1)
Use a concentration of 1 µg/ml.
ICC/IF (3)
1/400.
特記事項
WB
Use a concentration of 1 µg/ml.
ICC/IF
1/400.

ターゲット情報

  • 機能

    Seems to be a major component of sperm tail outer dense fibers (ODF). ODFs are filamentous structures located on the outside of the axoneme in the midpiece and principal piece of the mammalian sperm tail and may help to maintain the passive elastic structures and elastic recoil of the sperm tail. May have a modulating influence on sperm motility. Functions as a general scaffold protein that is specifically localized at the distal/subdistal appendages of mother centrioles. Component of the centrosome matrix required for the localization of PLK1 and NIN to the centrosomes. Required for the formation and/or maintenance of normal CETN1 assembly.

  • 組織特異性

    Testis-specific (at protein level). Highly expressed in cytoplasm of step 2 round spermatids. Detected in the middle piece and extends to about half the principal piece of the sperm tails.

  • 配列類似性

    Belongs to the ODF2 family.

  • 翻訳後修飾

    Tyrosine phosphorylated.

  • 細胞内局在

    Cytoplasm > cytoskeleton > centrosome. Cell projection > cilium. Cytoplasm > cytoskeleton > centrosome > centriole. Cytoplasm > cytoskeleton > spindle pole. Localized at the microtubule organizing centers in interphase and spindle poles in mitosis. Localized at the distal/subdistal appendages of mother centrioles.
  • Information by UniProt

  • 参照データベース

    • 別名

      • 84 kDa outer dense fiber protein antibody
      • Cancer/testis antigen 134 antibody
      • Cenexin 1 antibody
      • Cenexin antibody
      • Cenexin1 variant 1 antibody
      • CT134 antibody
      • FLJ44866 antibody
      • KKT 4 antibody
      • KKT4 antibody
      • MGC111096 antibody
      • MGC9034 antibody
      • ODF 2 antibody
      • ODF 84 antibody
      • odf2 antibody
      • ODF2/1 antibody
      • ODF2/2 antibody
      • ODF84 antibody
      • ODFP2_HUMAN antibody
      • OTTHUMP00000022274 antibody
      • OTTHUMP00000022275 antibody
      • Outer dense fiber of sperm tail 2 antibody
      • Outer dense fiber of sperm tails 2 antibody
      • Outer dense fiber of sperm tails protein 2 antibody
      • Outer dense fiber of sperm tails, 84 kD antibody
      • Outer dense fiber protein 2 antibody
      • Sperm outer dense fiber major protein 2 antibody
      • Sperm tail structural protein antibody
      • Testis specific autoantigen antibody
      see all

    画像

    • Western blot - Anti-Cenexin1/ODF2 antibody (ab43840)
      Western blot - Anti-Cenexin1/ODF2 antibody (ab43840)
      All lanes : ab43840 at 1 µg/ml

      Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 25 µg
      Lane 2 : MCF7 (Human breast adenocarcinoma) at 10 µg

      Secondary
      All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) (ab65484) at 1/3000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 93 kDa
      Observed band size: 100 kDa why is the actual band size different from the predicted?


      Exposure time: 20 minutes


      Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.

    • Immunocytochemistry/ Immunofluorescence - Anti-Cenexin1/ODF2 antibody (ab43840)
      Immunocytochemistry/ Immunofluorescence - Anti-Cenexin1/ODF2 antibody (ab43840)This image is courtesy of an Abreview submitted by Kirk McManus, Univ. of Manitoba/Cancer Care MICB.

      Immunocytochemistry/ Immunofluorescence analysis of human HCT116 cells labeling Cenexin1/ODF2 with ab43840 at 1/400 dilution. Cells were fixed in formaldehyde and permeabilized with 0.5% Triton X-100 in PBS. Staining with ab43840 at 1/400 was carried out for 1 hour at 22°C in PBS buffer. ab150081, a Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed secondary antibody was used at 1/200 dilution. DAPI was used to counterstain.

      See Abreview

    • Western blot - Anti-Cenexin1/ODF2 antibody (ab43840)
      Western blot - Anti-Cenexin1/ODF2 antibody (ab43840)Image courtesy of an anonymous abreview.
      All lanes : Anti-Cenexin1/ODF2 antibody (ab43840) at 1/500 dilution

      Lane 1 : 25 ug HeLa cell lysate
      Lane 2 : 12.5 ug HeLa cell lysate
      Lane 3 : 25 ug HeLa cell lysate with Human Cenexin1/ODF2 peptide (ab43839) at 4 µg/ml
      Lane 4 : 12.5 ug HeLa cell lysate with Human Cenexin1/ODF2 peptide (ab43839) at 4 µg/ml

      Secondary
      All lanes : Anti-Rabbit IgG HRP-conjugated at 1/5000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 93 kDa
      Observed band size: ~75 kDa why is the actual band size different from the predicted?


      Exposure time: 4 minutes


      Detection of Cenexin 1 by ab43840 in Western blot of HeLa cell lysates. Please see anonymous abreview for further details.

      See Abreview

    参考文献 (36)

    ab43840 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab43840 は 36 報の論文で使用されています。

    • Uzbas F & O'Neill AC Spatial Centrosome Proteomic Profiling of Human iPSC-derived Neural Cells. Bio Protoc 13:e4812 (2023). PubMed: 37727868
    • Ali A  et al. Microtubule nucleation and γTuRC centrosome localization in interphase cells require ch-TOG. Nat Commun 14:289 (2023). PubMed: 36702836
    • Zhu ZJ  et al. Novel mutation in ODF2 causes multiple morphological abnormalities of the sperm flagella in an infertile male. Asian J Androl 24:463-472 (2022). PubMed: 35102900
    • Magistrati E  et al. Myosin VI regulates ciliogenesis by promoting the turnover of the centrosomal/satellite protein OFD1. EMBO Rep 23:e54160 (2022). PubMed: 34957672
    • Klebanovych A  et al. C53 Interacting with UFM1-Protein Ligase 1 Regulates Microtubule Nucleation in Response to ER Stress. Cells 11:N/A (2022). PubMed: 35159364
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-9 of 9 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Cenexin1/ODF2 antibody

     Excellent
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HCT116)
    Permeabilization
    Yes - 0.5% Triton-X100 in PBS
    Specification
    HCT116
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Kirk Mcmanus

    Verified customer

    投稿 Jan 16 2017

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Cenexin1/ODF2 antibody

     Poor
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Specification
    HeLa
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.1% Triton
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: RT°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Jun 22 2011

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Cenexin1/ODF2 antibody

     Excellent
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Specification
    HeLa
    Fixative
    Formaldehyde
    Permeabilization
    Yes - PBS, 0.1% Triton
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 21°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Apr 08 2010

    Western blot abreview for Anti-Cenexin1/ODF2 antibody

     Good
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HeLa)
    Loading amount
    25 µg
    Specification
    HeLa
    Treatment
    4 ug/ml immunogen peptide
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Apr 08 2010

    Question

    Hello,
    I have attached the images mentioned in my previous letter. They illustrate my concern with this antibody. I will try to answer your specific questions:
    1. Are you staining fixed tissues (i.e. paraffin-embedded or frozen) or cells? What is the fixative used and fixation protocol?
    I it was tissue fixated in 3.7% paraformaldehyde for 30'.
    2. Is there a reason why you used this antibody instead of one of our centrosome markers like Ninein or Gamma tubulin?
    This antibody reportedly recognized the protein associated with basal foot of the basal body
    Ref1), I needed to resolve this particular structure within the basal body.
    3. According to SwissProt, the tissue specificity for this protein is testis-specific (at protein level). We also list HeLa cells as a positive control. Have you tested any of these controls?
    I am currently awaiting the results of this experiment and may contact you again once we get them.
    4.What had you tried for blocking?
    5. What was the dilution of the primary antibody used?
    The antibody was used in 1:200 dilution. At this dilution the antibody gives an excellent bright signal with almost no background, but the protein, which it recognizes, is localized most likely within the cilia axoneme, (above the apical cell surface) , and totally absent from the centrosomes, which are localized in the apical surface . It is unlikely that blocking may change this fact.
    Reference
    1. Cell. 2012 Jan 20;148(1-2):189-200.
    Coordinated ciliary beating requires Odf2-mediated polarization of basal bodies via basal feet.
    Kunimoto K, Yamazaki Y, Nishida T, Shinohara K, Ishikawa H, Hasegawa T, Okanoue T, Hamada H, Noda T, Tamura A, Tsukita S, Tsukita S.

    Read More
    Answer

    Thank you for your reply and for providing this additional information.
    Unfortunately the attachment didn't go through. Could you send it again compiled as 1 PDF, PPT, or DOC file?
    Please let me know what the results are for your control experiments. Right now it is difficult for us to determine why the antibody isn't working since the application IHC-P hasn't been tested and the tissue type is not validated. If you could run a positive control WB with HeLa or testis lysate, that would provide us with important information about whether or not the antibody is working.
    We look forward to hearing more information about the positive control.

    Read More

    Question

    Hello, With an aim to localize basal bodies/centrosomes in mouse trachea ciliated cells I have purchased recently the antibody to Anti-Cenexin1/ODF2 (ab43840). However, as it was shown on Fig1 , this Ab does not recognize centriole/ basal bodies in mouse trachea in IH experiments ( secondary antibody-anti rabbit Alexa A568 red , green –actin). It appears that Ab 43840 recognizes protein localized in cilia axoneme ,well above the cell apical surface , as it could be seen on the orthogonal view of the image shown on Fig 2. I ended up using the antibody purchased from Sigma that does recognize centrosomes, as it is shown on Fig 4 . For I cannot use the Ab 43840 for localization of centrosomes, as it was supposed to recognise , it renders it useless for my study . I would like ask you to issue me a credit to buy another antibody from your catalog that I can use for other aspects of my research . I hope that you find my request reasonable .

    Read More
    Answer

    Thank you for contacting us. I am sorry to hear that this antibody is not providing satisfactory results. The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality.
    You said that you're staining mouse trachea ciliated cells. Are you staining fixed tissues (i.e. paraffin-embedded or frozen) or cells? What is the fixative used and fixation protocol?
    According to SwissProt, the tissue specificity for this protein is testis-specific (at protein level). We also list HeLa cells as a positive control. Have you tested any of these controls?
    Was the antibody that worked from the other vendor also to this target protein, or was it to a different protein? Is there a reason why you used this antibody instead of one of our centrosome markers like Ninein or Gamma tubulin?
    What had you tried for blocking?
    What was the dilution of the primary antibody used?
    Should the suggestions not improve the results, please do let me know.
    In the event that a product is not functioning in the species and applications cited on the product datasheet (and the problem has been reported within 6 months of purchase), we would be pleased to provide a free of charge replacement, credit note, or refund.
    I hope this information is helpful, and I thank you for your cooperation.

    Read More

    Question

    Dear xxxx,

    Thank you very much for arranging everything, it's much appreciated. The attached pdf has details regarding the Ab and some images.

    Many Thanks

    Read More
    Answer

    Thank you very much for providing that information for me. It will be very helpful to us.

    I have just had a look at the status of your order of ab43840 and it has been shipped today so will therefore be with you tomorrow as I said. Please do let me know how you get on with this antibody.

    Until then, I wish you all the best with your research.

    Read More

    Question

    Dear xxxxxx,

    Thank you very much for the reply and am happy to know that you will able to provide me with an alternative.
    My address is partially correct so please use the one as follows:

    xxxxxxx
    I will be happy to send you images and additional details ASAP.
    Many Thanks for your support.

    Read More
    Answer

    Thank you for confirming those details.

    I have now arranged for the new vial ofab43840 to be sent out to you. This is on the order number 1097435(Purchase order number FOCR 303778). This should get to you tomorrow. If you have any problems receiving it please dolet me know.

    As mentioned in my previous email, it would be very useful for us to look into the problems you have had with ab72505. To this end, if you could share with me the protocol details and some images of the results I would be very grateful.

    If you encounterany further problemswith the new antibody ab43840 please dolet me know. I look forward to receiving your reply.

    Read More

    Question

    Has been stainingcentrioles but sees no differentiation between mature and non-mature as should be seen with this ab.

    Read More
    Answer

    Thank you for contacting us yesterday and sorry for the delay in getting back to you.

    I was sorry to hear that youhavebeenhavingdifficulties with the anti-CEP170 antibody(ab43840). We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

    As discussed over the phone, I would be happy to arrange forthereplacement antibody you requested ()to be sent to you. Could you please just confirm for me that the delivery address that I have for you is correct:

    >Once I have this confirmation I will arrange for the antibody ab43840 to be sent out to you.

    I understand the problems you have encountered with this antibody and the optimisation you have had to perform in trying to make it work as expected. If possible, so that I can investigate this case further with thelab, would you be able to give me a little more detail about the experiment carried out? If you wouldn't mind could you please fill out the questionnaire I have attached to this email, including any images you have if possible. This information will also allowme to investigate this case internally and initiate additional testing where necessary.

    I look forward to receiving your reply.

    Read More

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