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リコンビナントRabMAb

Anti-CEND1 抗体 [EPR3739] (ab113076)

Reviews (1) Submit a question References (3)
Western blot - Anti-CEND1 antibody [EPR3739] (ab113076)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEND1 antibody [EPR3739] (ab113076)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEND1 antibody [EPR3739] (ab113076)
  • Anti-CEND1 antibody [EPR3739] (ab113076)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3739] to CEND1
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-CEND1 antibody [EPR3739]
    CEND1 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR3739] to CEND1

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IHC-Pmore details
    適用なし: Flow Cyt

  • 種交差性

    交差種: Mouse, Human
    交差が予測される動物種: Rat

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • Human fetal brain lysate, U-87 MG and Neuro 2a cell lysates; Human brain glioma tissues.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab113076の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/10000 - 1/50000. Detects a band of approximately 22 kDa (predicted molecular weight: 15 kDa).
IHC-P
1/500 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min
特記事項
WB
1/10000 - 1/50000. Detects a band of approximately 22 kDa (predicted molecular weight: 15 kDa).
IHC-P
1/500 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min
追加情報
Is unsuitable for Flow Cyt.

ターゲット情報

  • 関連性

    CEND1 is a neuron-specific protein. The similar protein in pig enhances neuroblastoma cell differentiation in vitro and may be involved in neuronal differentiation in vivo. Multiple pseudogenes have been reported for this gene.

  • 細胞内局在

    Membrane; Single-pass type IV membrane protein.

  • 参照データベース

    • 別名

      • BM88 antibody
      • BM88 antigen antibody
      • Cell cycle exit and neuronal differentiation 1 antibody
      • Cell cycle exit and neuronal differentiation protein 1 antibody
      • FLJ90066 antibody
      • MGC34326 antibody
      see all

    画像

    • Western blot - Anti-CEND1 antibody [EPR3739] (ab113076)
      Western blot - Anti-CEND1 antibody [EPR3739] (ab113076)
      All lanes : Anti-CEND1 antibody [EPR3739] (ab113076) at 1/10000 dilution

      Lane 1 : Human fetal brain tissue lysate
      Lane 2 : U-87 MG cell lysate
      Lane 3 : Neuro-2a cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 15 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEND1 antibody [EPR3739] (ab113076)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEND1 antibody [EPR3739] (ab113076)

      ab113076 at 1/500 dilution, staining CEND1 in Praffin-embedded Human brain tissue by Immunohistochemistry.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEND1 antibody [EPR3739] (ab113076)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEND1 antibody [EPR3739] (ab113076)

      ab113076 at 1/500 dilution, staining CEND1 in Praffin-embedded Human glioma tissue by Immunohistochemistry.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Anti-CEND1 antibody [EPR3739] (ab113076)
      Anti-CEND1 antibody [EPR3739] (ab113076)

    参考文献 (3)

    ab113076 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab113076 は 3 報の論文で使用されています。

    • Wang R  et al. Cell cycle exit and neuronal differentiation 1-engineered embryonic neural stem cells enhance neuronal differentiation and neurobehavioral recovery after experimental traumatic brain injury. Neural Regen Res 17:130-136 (2022). PubMed: 34100448
    • Zhang L  et al. Loss of ZC4H2 and RNF220 Inhibits Neural Stem Cell Proliferation and Promotes Neuronal Differentiation. Cells 9:N/A (2020). PubMed: 32630355
    • Scaturro P  et al. An orthogonal proteomic survey uncovers novel Zika virus host factors. Nature 561:253-257 (2018). PubMed: 30177828

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    Immunohistochemistry (Frozen sections) abreview for Anti-CEND1 antibody [EPR3739]

     Excellent
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 22°C
    Sample
    Rat Tissue sections (retina of rat at birth)
    Specification
    retina of rat at birth
    Permeabilization
    Yes - 0.1% Triton
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    MRS. Claudine Botteri

    Verified customer

    投稿 Feb 25 2014

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com