Anti-Cellubrevin 抗体 [EPR16866] - BSA and Azide free

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Cellubrevin antibody [EPR16866] - BSA and Azide free (AB251315)

This data was developed using ab200657, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized A-375 (Human malignant melanoma cell line) cells labeling Cellubrevin with ab200657 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Cytoplasm staining on A-375 cell line is observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).

The negative controls are as follows :
-ve control 1 : ab200657 at 1/250 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
-ve control 2 : ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Cellubrevin antibody [EPR16866] - BSA and Azide free (AB251315)

This data was developed using ab200657, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized A549 (Human lung carcinoma cell line) cells labeling Cellubrevin with ab200657 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Cytoplasm staining on A549 cell line is observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).

The negative controls are as follows :
-ve control 1 : ab200657 at 1/250 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
-ve control 2 : ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Cellubrevin antibody [EPR16866] - BSA and Azide free (AB251315)

This data was developed using ab200657, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed A-375 (Human malignant melanoma cell line) cells labeling Cellubrevin with ab200657 at 1/60 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.

• IP

Supplier Data

Immunoprecipitation - Anti-Cellubrevin antibody [EPR16866] - BSA and Azide free (AB251315)

This data was developed using ab200657, the same antibody clone in a different buffer formulation.

Cellubrevin was immunoprecipitated from 1mg of A-375 (Human malignant melanoma cell line) whole cell lysate with ab200657 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab200657 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.
Lane 1 : A-375 whole cell lysate 10μg (Input).
Lane 2 : ab200657 IP in A-375 whole cell lysate.
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab200657 in A-375 whole cell lysate.
Blocking and dilution buffer and concentration : 5% NFDM/TBST.
Exposure time : 5 seconds.

All lanes:

Immunoprecipitation - Anti-Cellubrevin antibody [EPR16866] (<a href='/products/primary-antibodies/cellubrevin-antibody-epr16866-ab200657'>ab200657</a>)

Predicted band size: 11 kDa

false

• WB

Supplier Data

Western blot - Anti-Cellubrevin antibody [EPR16866] - BSA and Azide free (AB251315)

This data was developed using ab200657, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Cellubrevin antibody [EPR16866] (<a href='/products/primary-antibodies/cellubrevin-antibody-epr16866-ab200657'>ab200657</a>) at 1/2000 dilution

All lanes:

Human fetal lung lysate at 20 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 11 kDa

Observed band size: 11 kDa

false

Exposure time: 30s

• WB

Supplier Data

Western blot - Anti-Cellubrevin antibody [EPR16866] - BSA and Azide free (AB251315)

This data was developed using ab200657, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Cellubrevin antibody [EPR16866] (<a href='/products/primary-antibodies/cellubrevin-antibody-epr16866-ab200657'>ab200657</a>) at 1/10000 dilution

All lanes:

A-375 (Human malignant melanoma cell line) whole cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 11 kDa

Observed band size: 11 kDa

false

Exposure time: 5s

• WB

Supplier Data

Western blot - Anti-Cellubrevin antibody [EPR16866] - BSA and Azide free (AB251315)

This data was developed using ab200657, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Cellubrevin antibody [EPR16866] (<a href='/products/primary-antibodies/cellubrevin-antibody-epr16866-ab200657'>ab200657</a>) at 1/10000 dilution

All lanes:

Human placenta tissue lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 11 kDa

Observed band size: 11 kDa

false

Exposure time: 5s