Anti-CEBP Beta 抗体 (ab53138)
Key features and details
- Rabbit polyclonal to CEBP Beta
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
-
製品名
Anti-CEBP Beta antibody
CEBP Beta 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to CEBP Beta -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Mouse, Human -
免疫原
A synthetic non-phosphopeptide derived from human CEBP Beta around the phosphorylation site of threonine 235 (P-G-T-P-S).
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 0.87% Sodium chloride, 50% Glycerol, PBS
Without Mg+2 and Ca+2 -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab53138の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/300 - 1/1000. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).
|
|
| IHC-P |
Use at an assay dependent concentration.
|
| 特記事項 |
|---|
|
WB
1/300 - 1/1000. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa). |
|
IHC-P
Use at an assay dependent concentration. |
ターゲット情報
-
機能
Important transcriptional activator in the regulation of genes involved in immune and inflammatory responses. Specifically binds to an IL-1 response element in the IL-6 gene. NF-IL6 also binds to regulatory regions of several acute-phase and cytokines genes. It probably plays a role in the regulation of acute-phase reaction, inflammation and hemopoiesis. The consensus recognition site is 5'-T[TG]NNGNAA[TG]-3'. Functions in brown adipose tissue (BAT) differentiation. -
組織特異性
Expressed at low levels in the lung, kidney and spleen. -
配列類似性
Belongs to the bZIP family. C/EBP subfamily.
Contains 1 bZIP domain. -
ドメイン
the 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors. -
翻訳後修飾
Sumoylated by polymeric chains of SUMO2 or SUMO3. -
細胞内局在
Nucleus. - Information by UniProt
-
参照データベース
- Entrez Gene: 1051 Human
- Entrez Gene: 12608 Mouse
- Omim: 189965 Human
- SwissProt: P17676 Human
- SwissProt: P28033 Mouse
- Unigene: 517106 Human
- Unigene: 719041 Human
- Unigene: 720603 Human
see all -
別名
- AGP/EBP antibody
- C EBP beta antibody
- C/EBP beta antibody
see all
画像
-
Western blot - Anti-CEBP Beta antibody (ab53138)All lanes : Anti-CEBP Beta antibody (ab53138) at 1/300 dilution
Lane 1 : NIH/3T3 cell extract
Lane 2 : NIH/3T3 cell extract with immunizing peptide
Predicted band size: 36 kDa
Observed band size: 36 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEBP Beta antibody (ab53138)ab53138 at 1/50 dilution staining CEBP Beta in human lung carcinoma by Immunohistochemistry, Paraffin embedded tissue, in the absence or presence of the immunising peptide.
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (9)
ab53138 は 9 報の論文で使用されています。
- Feng Y et al. MicroRNA-376b-3p Suppresses Choroidal Neovascularization by Regulating Glutaminolysis in Endothelial Cells. Invest Ophthalmol Vis Sci 64:22 (2023). PubMed: 36719700
- Shi S et al. Andrographolide in atherosclerosis: integrating network pharmacology and in vitro pharmacological evaluation. Biosci Rep 42:N/A (2022). PubMed: 35543243
- Chu S et al. C-EBPβ mediates in cigarette/IL-17A-induced bronchial epithelial-mesenchymal transition in COPD mice. BMC Pulm Med 21:376 (2021). PubMed: 34794427
- Gao J et al. LncRNA LEF1-AS1 silencing diminishes EZH2 expression to delay hepatocellular carcinoma development by impairing CEBPB-interaction with CDCA7. Cell Cycle 19:870-883 (2020). PubMed: 32178558
- Li F et al. MicroRNA-191 targets CCAAT/enhanced binding protein ß and functions as an oncogenic molecule in human non-small cell lung carcinoma cells. Exp Ther Med 18:1175-1183 (2019). PubMed: 31316611
- Gao S et al. Effects of the combination of tanshinone IIA and puerarin on cardiac function and inflammatory response in myocardial ischemia mice. J Mol Cell Cardiol 137:59-70 (2019). PubMed: 31629735
- Richter M et al. Pancreatic progenitor-derived hepatocytes are viable and functional in a 3D high density bioreactor culture system. Toxicol Res (Camb) 5:278-290 (2016). PubMed: 30090344
- Mir RA & Chauhan SS Down Regulation of a Matrix Degrading Cysteine Protease Cathepsin L, by Acetaldehyde: Role of C/EBPa. PLoS One 6:e20768 (2011). WB ; Human . PubMed: 21687683
- Younce CW et al. MCP-1 (monocyte chemotactic protein-1)-induced protein, a recently identified zinc finger protein, induces adipogenesis in 3T3-L1 pre-adipocytes without peroxisome proliferator-activated receptor gamma. J Biol Chem 284:27620-8 (2009). WB ; Mouse . PubMed: 19666473