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リコンビナントRabMAb

Anti-CEBP Alpha/CEBPA 抗体 [EP708Y] (ab40761)

Reviews (2)Q&A (1)References (19)
Western blot - Anti-CEBP Alpha/CEBPA antibody [EP708Y] (ab40761)
  • Immunocytochemistry/ Immunofluorescence - Anti-CEBP Alpha/CEBPA antibody [EP708Y] (ab40761)
  • Flow Cytometry (Intracellular) - Anti-CEBP Alpha/CEBPA antibody [EP708Y] (ab40761)
  • Western blot - Anti-CEBP Alpha/CEBPA antibody [EP708Y] (ab40761)
  • Anti-CEBP Alpha/CEBPA antibody [EP708Y] (ab40761)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP708Y] to CEBP Alpha/CEBPA
  • Suitable for: Flow Cyt (Intra), WB, ICC/IF
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-CEBP Alpha/CEBPA antibody [EP708Y]
    CEBP Alpha/CEBPA 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EP708Y] to CEBP Alpha/CEBPA

  • 由来種

    Rabbit

  • 特異性

    This antibody recognizes isoform1 and isoform 4.

  • アプリケーション

    適用あり: Flow Cyt (Intra), WB, ICC/IFmore details
    適用なし: IHC or IP

  • 種交差性

    交差種: Human

  • 免疫原

    Synthetic peptide within Human CEBP Alpha/CEBPA aa 1-100 (N terminal). The exact sequence is proprietary.

  • ポジティブ･コントロール

    • U937+LPS cell lysate, HeLa cells.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab40761の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt (Intra)
1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB (2)
1/1000 - 1/10000. Detects a band of approximately 30,43 kDa (predicted molecular weight: 43 kDa).
ICC/IF
1/250 - 1/500.
特記事項
Flow Cyt (Intra)
1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/1000 - 1/10000. Detects a band of approximately 30,43 kDa (predicted molecular weight: 43 kDa).
ICC/IF
1/250 - 1/500.
追加情報
Is unsuitable for IHC or IP.

ターゲット情報

  • 機能

    C/EBP is a DNA-binding protein that recognizes two different motifs: the CCAAT homology common to many promoters and the enhanced core homology common to many enhancers.

  • 配列類似性

    Belongs to the bZIP family. C/EBP subfamily.
    Contains 1 bZIP domain.

  • 細胞内局在

    Nucleus.
  • Information by UniProt

  • 参照データベース

    • 別名

      • Apoptotic cysteine protease antibody
      • Apoptotic protease Mch 5 antibody
      • C/EBP alpha antibody
      • C/ebpalpha antibody
      • CAP4 antibody
      • Caspase 8 precursor antibody
      • CBF-A antibody
      • CCAAT Enhancer Binding Protein alpha antibody
      • CCAAT/enhancer binding protein (C/EBP), alpha antibody
      • CCAAT/enhancer-binding protein alpha antibody
      • CEBP antibody
      • CEBP A antibody
      • CEBP alpha antibody
      • Cebpa antibody
      • CEBPA_HUMAN antibody
      • FADD homologous ICE/CED 3 like protease antibody
      • FADD like ICE antibody
      • FLICE antibody
      • ICE like apoptotic protease 5 antibody
      • ICE8 antibody
      • MACH antibody
      • MCH5 antibody
      • MORT1 associated CED 3 homolog antibody
      see all

    画像

    • Western blot - Anti-CEBP Alpha/CEBPA antibody [EP708Y] (ab40761)
      Western blot - Anti-CEBP Alpha/CEBPA antibody [EP708Y] (ab40761)
      Anti-CEBP Alpha/CEBPA antibody [EP708Y] (ab40761) at 1/1000 dilution + U937 + LPS cell lysate at 10 µg

      Predicted band size: 43 kDa
      Observed band size: 30,43 kDa why is the actual band size different from the predicted?



      We are unsure as to the identity of the 30kda band.

    • Immunocytochemistry/ Immunofluorescence - Anti-CEBP Alpha/CEBPA antibody [EP708Y] (ab40761)
      Immunocytochemistry/ Immunofluorescence - Anti-CEBP Alpha/CEBPA antibody [EP708Y] (ab40761)
      ab40761 (1/250) staining HeLa cells using immunofluorescence.
    • Flow Cytometry (Intracellular) - Anti-CEBP Alpha/CEBPA antibody [EP708Y] (ab40761)
      Flow Cytometry (Intracellular) - Anti-CEBP Alpha/CEBPA antibody [EP708Y] (ab40761)

      Overlay histogram showing HeLa cells stained with ab40761 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab40761, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. 

       

       

    • Western blot - Anti-CEBP Alpha/CEBPA antibody [EP708Y] (ab40761)
      Western blot - Anti-CEBP Alpha/CEBPA antibody [EP708Y] (ab40761)This image is courtesy of an anonymous Abreview
      Anti-CEBP Alpha/CEBPA antibody [EP708Y] (ab40761) at 1/5000 dilution + Human MCF-7 whole cell lysate at 100000 cells

      Secondary
      HRP-conjugated Donkey anti-rabbit IgG polyclonal at 1/2000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 43 kDa
      Observed band size: 40 kDa why is the actual band size different from the predicted?


      Exposure time: 1 minute


      Blocked with 5% Milk for 1 hour at 24°C.

      We are unsure as to the identity of the 30kda band.

      See Abreview

    • Anti-CEBP Alpha/CEBPA antibody [EP708Y] (ab40761)
      Anti-CEBP Alpha/CEBPA antibody [EP708Y] (ab40761)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (19)

    ab40761 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab40761 は 19 報の論文で使用されています。

    • Dong X  et al. METTL14 mediates m6a modification on osteogenic proliferation and differentiation of bone marrow mesenchymal stem cells by regulating the processing of pri-miR-873. Mol Med Rep 28:N/A (2023). PubMed: 37449516
    • Zhang LM  et al. CCAAT enhancer binding protein α suppresses proliferation, metastasis, and epithelial-mesenchymal transition of ovarian cancer cells via suppressing the Wnt/β-catenin signaling. Neoplasma 68:602-612 (2021). PubMed: 33780265
    • Ni T  et al. Dihydrolycorine Attenuates Cardiac Fibrosis and Dysfunction by Downregulating Runx1 following Myocardial Infarction. Oxid Med Cell Longev 2021:8528239 (2021). PubMed: 34725565
    • Pope BD  et al. Fattening chips: hypertrophy, feeding, and fasting of human white adipocytes in vitro. Lab Chip 20:4152-4165 (2020). PubMed: 33034335
    • Zhang W  et al. A novel peptide RIFV suppresses human adipocyte differentiation through the inhibition of C/EBP-ß expression. Nutr Metab (Lond) 16:88 (2019). PubMed: 31889968
    View all Publications for this product

    レビューと Q&A

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    レビューを送る 質問を送る

    1-3 of 3 Abreviews or Q&A

    Western blot abreview for Anti-CEBP Alpha/CEBPA antibody [EP708Y]

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (MCF7)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    100000 cells
    Specification
    MCF7
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 May 11 2012

    Western blot abreview for Anti-CEBP Alpha/CEBPA antibody [EP708Y]

     Average
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HT1080 Cell line)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    200000 cells
    Specification
    HT1080 Cell line
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Jan 04 2010

    Question

    Hello, I am interested in your anti-CEBPalpha antibody (ab40761). I noticed the immunofluorescence and flow cytometry are done in HeLa cells but not the Western blot. Could you provide a Western blot of HeLa cells using this antibody? This is one of few cell types I'd be using the antibody in; I would like to see how clean it is. Please let me know. Thank you so much.

    Read More
    Answer

    Thank you for contacting Abcam.

    We do not currently have an image of how ab40761 works in western blot on HeLa cells, all of the western blot data we have for this antibody is on our website. The antibody is covered under our Abpromise for six months and is guaranteed to work in western blot on human samples.

    If there is anything else I can help you with, please let me know.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com