Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online

Anti-CEBP Alpha/CEBPA 抗体 [15C8] (ab15047)

Submit a review Q&A (5)References (5)
Western blot - Anti-CEBP Alpha/CEBPA antibody [15C8] (ab15047)

    Key features and details

    • Mouse monoclonal [15C8] to CEBP Alpha/CEBPA
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    リコンビナント抗体で、ロット間での高い再現性を実現

    Product image
    Anti-CEBP Alpha/CEBPA antibody [EPR27924-90] (ab317442)
    • 異なるロット間での安定した再現性
    • 容易なスケールアップ
    • 評価試験による特異性の確認済み
    • 倫理基準に準拠 - アニマル・フリーの生産

    製品の概要

    • 製品名

      Anti-CEBP Alpha/CEBPA antibody [15C8]
      CEBP Alpha/CEBPA 一次抗体 製品一覧

    • 製品の詳細

      Mouse monoclonal [15C8] to CEBP Alpha/CEBPA

    • 由来種

      Mouse

    • 特異性

      This antibody may cross react with glutamate--ammonia ligase domain containing 1 protein ((Lengsin) Lens glutamine synthase-like) as it has 8/10 aa identical to the immunogen used to generate ab15047 (this protein is part of the Glutamate synthetase family so its compartmentalization to the cytoplasm) unlike CEDPA which is found in the nucleus.

    • アプリケーション

      適用あり: WBmore details

    • 種交差性

      交差種: Human
      交差が予測される動物種: Hamster, Cow, Xenopus laevis, Schizosaccharomyces pombe, Zebrafish, Goldfish, Aspergillus nidulans, Neurospora crassa

    • 免疫原

      Synthetic peptide corresponding to Mouse CEBP Alpha/CEBPA aa 1-14.
      Sequence:

      MESADFYEVEPRPP


      (Peptide available as ab39791)
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

    • エピトープ

      Amino terminal region.

    • ポジティブ･コントロール

      • Transfected hu293T cells.

    • 特記事項

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    製品の特性

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab15047の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    WB
    Use a concentration of 2 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).
    特記事項
    WB
    Use a concentration of 2 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).

    ターゲット情報

    • 機能

      C/EBP is a DNA-binding protein that recognizes two different motifs: the CCAAT homology common to many promoters and the enhanced core homology common to many enhancers.

    • 配列類似性

      Belongs to the bZIP family. C/EBP subfamily.
      Contains 1 bZIP domain.

    • 細胞内局在

      Nucleus.
    • Information by UniProt

    • 参照データベース

      • 別名

        • Apoptotic cysteine protease antibody
        • Apoptotic protease Mch 5 antibody
        • C/EBP alpha antibody
        • C/ebpalpha antibody
        • CAP4 antibody
        • Caspase 8 precursor antibody
        • CBF-A antibody
        • CCAAT Enhancer Binding Protein alpha antibody
        • CCAAT/enhancer binding protein (C/EBP), alpha antibody
        • CCAAT/enhancer-binding protein alpha antibody
        • CEBP antibody
        • CEBP A antibody
        • CEBP alpha antibody
        • Cebpa antibody
        • CEBPA_HUMAN antibody
        • FADD homologous ICE/CED 3 like protease antibody
        • FADD like ICE antibody
        • FLICE antibody
        • ICE like apoptotic protease 5 antibody
        • ICE8 antibody
        • MACH antibody
        • MCH5 antibody
        • MORT1 associated CED 3 homolog antibody
        see all

      画像

      • Western blot - Anti-CEBP Alpha/CEBPA antibody [15C8] (ab15047)
        Western blot - Anti-CEBP Alpha/CEBPA antibody [15C8] (ab15047)
        Anti-CEBP Alpha/CEBPA antibody [15C8] (ab15047) at 2 µg/ml + 293 cell lysates transfected with CEBP alpha.

        Predicted band size: 45 kDa

      参考文献 (5)

      ab15047 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab15047 は 5 報の論文で使用されています。

      • Bai R  et al. Expression of NFIL3 and CEBPA regulated by IFNT induced-PGE2 in bovine endometrial stromal cells during the pre-implantation period. Front Endocrinol (Lausanne) 14:1075030 (2023). PubMed: 36923228
      • You L  et al. Extract of Acalypha australis L. inhibits lipid accumulation and ameliorates HFD-induced obesity in mice through regulating adipose differentiation by decreasing PPAR? and CEBP/a expression. Food Nutr Res 65:N/A (2021). PubMed: 33776618
      • Xu Q  et al. All-trans retinoic acid controls differentiation, proliferation, and lipolysis in isolated subcutaneous adipocytes from peripartal Holstein cows. J Dairy Sci 104:4999-5008 (2021). PubMed: 33551168
      • Meng X  et al. Heterogeneous nuclear ribonucleoprotein A1 interacts with microRNA-34a to promote chondrogenic differentiation of mesenchymal stem cells. Am J Transl Res 9:1774-1782 (2017). WB ; Mouse . PubMed: 28469782
      • Khanna-Gupta A  et al. Up-regulation of Translation Eukaryotic Initiation Factor 4E in Nucleophosmin 1 Haploinsufficient Cells Results in Changes in CCAAT Enhancer-binding Protein a Activity: IMPLICATIONS IN MYELODYSPLASTIC SYNDROME AND ACUTE MYELOID LEUKEMIA. J Biol Chem 287:32728-37 (2012). WB . PubMed: 22851180

      レビューと Q&A

      Show All レビュー Q&A
      レビューを送る 質問を送る

      1-5 of 5 Abreviews or Q&A

      Question

      The species of the gene that the plasmid contained is human.   I would be very grateful to you if you could replace cepb alpha mouse ab with rabbit cebp alpha ab40764.       

      Read More
      Answer

      Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number **** for ab40764. To check the status of the order please contact our Customer Service team and reference this number. Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know. I wish you the best of luck with your research.  

      Read More

      Question

      Thanks for the prompt answer. See my answers below please: 1.What is the species of the transfected CEBP alpha? -Human 293 T cells were transfected with C/ebp alpha plazmid  2.How much protein did you load per lane of the gel? -Approximately 70µg per lane   3.What was the dilution or concentration of the antibody that you incubated with the membrane? - 1:300   I also used your antibody for WB with IP samples and it showed no signal. If you are interested I could send you this WB result.

      Read More
      Answer

      Thank you for sending the additional details. You mentioned that the human cells were transfected with a plasmid; can you please tell me the species of the gene that the plasmid contained? Was this also human, or some other species, for instance mouse/rat, etc? I would like to replace the antibody for you but ab15047 is the only mouse antibody we have for CEBP alpha. Would you be interested in a rabbit monoclonal, for instance ab40761, or ab40764? Click here (or use the following: https://www.abcam.com/CEBP-AlphaCEBPA-antibody-EP708Y-ab40761.html). Click here (or use the following: https://www.abcam.com/CEBP-AlphaCEBPA-antibody-EP709Y-ab40764.html).

      Read More

      Question

      Recently I've contacted you because of CEBP Alpha antibody work failure.   I attached our standard protocol for WB procedure. Unfortunately we don't have word version, that's why I'm sending out a PDF format. I was using 10 % separating gel and 4 % stacking gel. The transfer time was 25 min.   I also attached the results of WB with 293 T cells transfected with Cebp alpha. First membrane #1 was incubated with your AB and no signal was obtained. Later I incubated the same membrane with Cebp alpha R and got a signal.   Interestingly, we also bought mouse Cebp alpha from Novus Biologicals (it has the same clone as yours: 15C8) and this AB doesn't work as well. I hope there's a possibility to return the AB or sabstitude this with another clone of Cebp alpha mouse AB. Anyway will be grateful to you for any tips you might give me.    

      Read More
      Answer

      Thank you for sending the protocol and image of your blot. I am sorry to see that you are not obtaining a band that corresponds to CEBP Alpha. I am having some difficulty reading the blot image notations. Can you please answer a few questions, to help me understand the problem? What is the species of the transfected CEBP alpha? How much protein did you load per lane of the gel? What was the dilution or concentration of the antibody that you incubated with the membrane? Finally, what was the antibody that produced the signal in lane four of the image you sent? I see your note, "Later I incubated the same membrane with Cebp alpha R and got a signal", but I am not sure what you mean by Cebp alpha R. Please also tell me your order number for ab15047. I look forward to your reply.

      Read More

      Question

      Many thanks for your e-mail.Following our conversation yesterday,I checked the expasy site and found the protein that you suggests cross reacts with the CEBP alpha antibody.The size ie 57KD fits perfectly with the size of the band that we see on a western blot.Also I have undertaked a 2D blot and obtained a very clean spot in the acidic region of the gel at about pI 5.0.The theorectical pI for the ligase is 5.9.This protein is part of the Glutamate synthetase family so its compartmentalization to the cytoplasm may indeed explain our data.We should be able to pull enough of the protein for mass spec analysis to be absolutely certain.When we do I will let you know,but you should warn your customers of the possable cross reactivities.

      Read More
      Answer

      Many thanks for your feedback, I have added a note on the datasheet following your expert advice and would love to find out if indeed the antibody detected the ligase in your cells. Thank you again for your help, I look forward to hearing from you,

      Read More

      Question

      The customer is detecting the expected 45kDa band in THP1 cells but a 50-55kDA band in B and T cell tumours (human), which is not found in a nuclear fraction but in a cytosolic fraction suggesting the antibody detects another protein or that the protein is abnormally expressed in the cytosol.

      Read More
      Answer

      As discussed earlier I enclose the links to the databases that I was looking at to find out if the antibody could be cross reacting with other proteins. The protein Glutamate--ammonia ligase domain containing 1 protein (Lengsin) Lens glutamine synthase-like) has 8/10 aa identical to the immunogen used to generate ab15047. The information can be found at: http://ca.expasy.org/cgi-bin/niceprot.pl?Q91VC6 I was unable to find any references to a cytoplasmic presence of CEBPA, sorry I couldn't be of more help, Please do not hesitate to contact me again if I can be of further assistance,

      Read More

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com