Anti-CEACAM6 抗体 [9A6] (ab78029)
![Immunocytochemistry/ Immunofluorescence - Anti-CEACAM6 antibody [9A6] (ab78029)](https://www.abcam.co.jp/ps/products/78/ab78029/Images/ab78029-312532-ab78029ICC.png "Immunocytochemistry/ Immunofluorescence - Anti-CEACAM6 antibody [9A6] (ab78029)")
Key features and details
- Mouse monoclonal [9A6] to CEACAM6
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG1
製品の概要
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製品名
Anti-CEACAM6 antibody [9A6]
CEACAM6 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [9A6] to CEACAM6 -
由来種
Mouse -
アプリケーション
適用あり: ICC/IF, WBmore details -
種交差性
交差種: Human -
免疫原
Tissue, cells or virus corresponding to Human CEACAM6. Tumor cell line expressing CEACAM6
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.09% Sodium azide
Constituents: 99.8% PBS, 0.1% BSA -
Concentration information loading... -
精製度
Protein G purified -
特記事項（精製）
ab78029 is purified. -
ポリ/モノ
モノクローナル -
クローン名
9A6 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab78029の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
1/100 - 1/200.
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| WB | (1) |
1/500. Predicted molecular weight: 37 kDa.
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| 特記事項 |
|---|
|
ICC/IF
1/100 - 1/200. |
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WB
1/500. Predicted molecular weight: 37 kDa. |
ターゲット情報
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配列類似性
Belongs to the immunoglobulin superfamily. CEA family.
Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
細胞内局在
Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 4680 Human
- Omim: 163980 Human
- SwissProt: P40199 Human
- Unigene: 466814 Human
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別名
- Carcinoembryonic antigen related cell adhesion molecule 6 antibody
- Carcinoembryonic antigen related cell adhesion molecule 6 (non specific cross reacting antigen) antibody
- Carcinoembryonic antigen-related cell adhesion molecule 6 antibody
see all
画像
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Immunocytochemistry/ Immunofluorescence - Anti-CEACAM6 antibody [9A6] (ab78029)
Immunofluorescence of THP-1 cells with (A) mouse IgG1, κ isotype control (Negative) or (B-D) CEACAM6 primary antibody (ab78029). Alexa Fluor® 488 (Green) Goat anti-Mouse IgG was used as secondary antibody. Nuclei were counterstained with DAPI. The image was captured with a 60X objective using KEYENCE BZ-X700 fluorescence microscope. Exposure time (Seconds) for (A) is 1/12, and (B-D) is 1/15. Concentrations for (A, B) is 1/100, (C) is 1/200 and (D) is 1:500.
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![Western blot - Anti-CEACAM6 antibody [9A6] (ab78029)](https://www.abcam.co.jp/ps/products/78/ab78029/Images/ab78029-312534-ab78029WB.png)
Western blot - Anti-CEACAM6 antibody [9A6] (ab78029)All lanes : Anti-CEACAM6 antibody [9A6] (ab78029) at 1/500 dilution
Lane 1 : HeLa
Lane 2 : THP-1
Lane 3 : HL-60 (Low expression negative control)
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : HRP Goat anti-Mouse
Predicted band size: 37 kDaHRP anti-β-actin was used as a loading control 1:8000
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (10)
ab78029 は 10 報の論文で使用されています。
- Burgos M et al. Prognostic value of the immune target CEACAM6 in cancer: a meta-analysis. Ther Adv Med Oncol 14:17588359211072621 (2022). PubMed: 35082925
- Chu C et al. Circ_0008035 promotes the progression of gastric cancer via the regulation of miR-1256/CEACAM6 axis. Cell Cycle 21:1091-1102 (2022). PubMed: 35220873
- Tian C et al. Effect of CEACAM6 silencing on the biological behavior of human gallbladder cancer cells. Oncol Lett 20:2677-2688 (2020). PubMed: 32782584
- Goldson TM et al. Nucleolin mediates the binding of cancer cells to L-selectin under conditions of lymphodynamic shear stress. Am J Physiol Cell Physiol 318:C83-C93 (2020). PubMed: 31644306
- Liu X et al. Heparanase Promotes Tumor Growth and Liver Metastasis of Colorectal Cancer Cells by Activating the p38/MMP1 Axis. Front Oncol 9:216 (2019). PubMed: 31001480
- Zang M et al. Dual role of carcinoembryonic antigen-related cell adhesion molecule 6 expression in predicting the overall survival of gastric cancer patients. Sci Rep 7:10773 (2017). IHC ; Human . PubMed: 28883649
- Jang MH et al. The Diagnostic Usefulness of HMGA2, Survivin, CEACAM6, and SFN/14-3-3 d in Follicular Thyroid Carcinoma. J Pathol Transl Med 49:112-7 (2015). PubMed: 25812733
- Zhang Y et al. CEACAM6 promotes tumor migration, invasion, and metastasis in gastric cancer. Acta Biochim Biophys Sin (Shanghai) 46:283-90 (2014). PubMed: 24492534
- Jarry A et al. Subversion of human intestinal mucosa innate immunity by a Crohn's disease-associated E. coli. Mucosal Immunol N/A:N/A (2014). IHC ; Human . PubMed: 25269707
- Balk-Møller E et al. A marker of endocrine receptor-positive cells, CEACAM6, is shared by two major classes of breast cancer: luminal and HER2-enriched. Am J Pathol 184:1198-208 (2014). IHC-Fr, ICC ; Human . PubMed: 24655379