Anti-CEACAM1 抗体 [EPR26490-88] (BSA and Azide free) (ab300062)
![Western blot - Anti-CEACAM1 antibody [EPR26490-88] (BSA and Azide free) (AB300062)](https://www.abcam.co.jp/ps/products/300/ab300062/Images/AB300062-486001-WB3.jpg "Western blot - Anti-CEACAM1 antibody [EPR26490-88] (BSA and Azide free) (AB300062)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR26490-88] to CEACAM1 - BSA and Azide free
- Suitable for: ICC/IF, IHC-P, WB, IP, Flow Cyt
- Knockout validated
- Reacts with: Human
製品の概要
-
製品名
Anti-CEACAM1 antibody [EPR26490-88] (BSA and Azide free)
CEACAM1 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR26490-88] to CEACAM1 - BSA and Azide free -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, IHC-P, WB, IP, Flow Cytmore details
適用なし: IHC-Fr -
種交差性
交差種: Human
非交差種: Mouse, Rat -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
-
ポジティブ･コントロール
- WB: Wild-type A549, HepG2, Human kidney, Human colon, A-204, SW480, His-tagged human CEACAM-1 recombinant protein aa35-428 recombinant protein lysates. IHC-P: Human: colon, endometrium, liver and hepatic carcinoma tissues ICC/IF: A549 cells. Flow cyt.: Wild-type A549 cell. IP: A549 cell.
-
特記事項
Ab300062 is a carrier free version of ab300061.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 7.2
Constituent: 100% PBS -
キャリア・フリー
はい -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR26490-88 -
アイソタイプ
IgG -
研究分野
関連製品
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab300062の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
Use at an assay dependent concentration.
|
|
| IHC-P |
Use at an assay dependent concentration.
|
|
| WB |
Use at an assay dependent concentration. Predicted molecular weight: 57 kDa.
|
|
| IP |
Use at an assay dependent concentration.
|
|
| Flow Cyt |
Use at an assay dependent concentration.
|
| 特記事項 |
|---|
|
ICC/IF
Use at an assay dependent concentration. |
|
IHC-P
Use at an assay dependent concentration. |
|
WB
Use at an assay dependent concentration. Predicted molecular weight: 57 kDa. |
|
IP
Use at an assay dependent concentration. |
|
Flow Cyt
Use at an assay dependent concentration. |
ターゲット情報
-
配列類似性
Belongs to the immunoglobulin superfamily. CEA family.
Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
細胞内局在
Secreted; Cell membrane and Cell membrane. Localizes to sites of cell-cell contact. - Information by UniProt
-
参照データベース
- Entrez Gene: 634 Human
- Omim: 109770 Human
- SwissProt: P13688 Human
- Unigene: 512682 Human
-
製品の状態
There are 11 isoforms produced by alternative splicing. Isoform 1 = BGPa; CEACAM1-4L; TM1-CEA; Isoform 2 = BGPg; CEACAM1-4C1; Isoform 3 = BGPh; CEACAM1-3; Isoform 4 = BGPi; CEACAM1-3C2; Isoform 5 = BGPy; CEACAM1-3AL; Isoform 6 = BGPb; CEACAM1-3L; TM2-CEA; Isoform 7 = BGPx; CEACAM1-1L; Isoform 8 = BGPc; CEACAM1-4S; TM3-CEA; Isoform 9 = BGPz; CEACAM1-3AS and Isoform 11 = BGPd; CEACAM1-3S. -
別名
- Antigen CD66 antibody
- BGP 1 antibody
- BGP antibody
see all
画像
-
Western blot - Anti-CEACAM1 antibody [EPR26490-88] (BSA and Azide free) (AB300062)All lanes : Anti-CEACAM1 antibody [EPR26490-88] (ab300061) at 1/1000 dilution
Lane 1 : His-tagged human CEACAM-1 recombinant protein aa (aa35-428) 20ng
Lane 2 : His-tagged human CEACAM-5/CD66e recombinant protein (aa35-685) 20ng
Lane 3 : His-tagged human CEACAM-6/CD66c recombinant protein (aa35-320) 20ng
Lane 4 : His-tagged human CEACAM-8/CD66b recombinant protein(aa35-320) 20ng
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 57 kDa
Observed band size: 100-180 kDa why is the actual band size different from the predicted?
Exposure time: 48 secondsThis data was developed using ab300061, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
-
![Western blot - Anti-CEACAM1 antibody [EPR26490-88] (BSA and Azide free) (AB300062)](https://www.abcam.co.jp/ps/products/300/ab300062/Images/AB300062-486000-WB2.jpg)
Western blot - Anti-CEACAM1 antibody [EPR26490-88] (BSA and Azide free) (AB300062)All lanes : Anti-CEACAM1 antibody [EPR26490-88] (ab300061) at 1/1000 dilution
Lane 1 : Human kidney tissue lysate
Lane 2 : Human colon tissue lysate
Lane 3 : A-204 (human muscle rhabdomyosarcoma) whole cell lysate
Lane 4 : SW480 (human colorectal adenocarcinoma epithelial cell) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Lanes 1-2 : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG (Merck DC03L) at 1/2000 dilution
Lanes 3-4 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 57 kDa
Observed band size: 100-180 kDa why is the actual band size different from the predicted?This data was developed using ab300061, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: Lanes 1-3:10 seconds; Lane 4: 48 seconds.
-
![Western blot - Anti-CEACAM1 antibody [EPR26490-88] (BSA and Azide free) (AB300062)](https://www.abcam.co.jp/ps/products/300/ab300062/Images/AB300062-485994-WB1.jpg)
Western blot - Anti-CEACAM1 antibody [EPR26490-88] (BSA and Azide free) (AB300062)All lanes : Anti-CEACAM1 antibody [EPR26490-88] (ab300061) at 1/1000 dilution
Lane 1 : Wild-type A549 (ab255450) whole cell lysate
Lane 2 : CEACAM1 knockout A549 (ab267047) whole cell lysate
Lane 3 : HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) at 1/10000 dilution
Predicted band size: 57 kDa
Observed band size: 100-180 kDa why is the actual band size different from the predicted?This data was developed using ab300061, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer and concentration: Intercept® (TBS) Blocking Buffer diluted with an equal volume of 0.1% TBS.
Lanes 1-3: Merged signal (red and green). Green - ab300061 observed at 100-180 kDa. Red - loading control ab8245 observed at 36 kDa.
ab300061 Anti-CEACAM1 antibody [EPR26490-88] was shown to specifically react with CEACAM1 in wild-type A549 cells (ab255450). Loss of signal was observed when knockout cell line (ab267047) was used. Wild-type and CEACAM1 knockout samples were subjected to SDS-PAGE. ab300061 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at 4? overnight at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEACAM1 antibody [EPR26490-88] (BSA and Azide free) (AB300062)](https://www.abcam.co.jp/ps/products/300/ab300062/Images/AB300062-2-anti-ceacam1-antibody-epr2649088-immunohistochemistry-human-colon.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEACAM1 antibody [EPR26490-88] (BSA and Azide free) (AB300062)This data was developed using ab300061, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human colon tissue labelling CEACAM1 with ab300061 at 1/2000 followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). Positive staining on human colon (PMID: 8543780). The section was incubated with ab300061 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: PBS was used instead of primary antibody (ab300061) followed by ready to use secondary antibody LeicaDS9800 (BOND™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEACAM1 antibody [EPR26490-88] (BSA and Azide free) (AB300062)](https://www.abcam.co.jp/ps/products/300/ab300062/Images/AB300062-3-anti-ceacam1-antibody-epr2649088-immunohistochemistry-human-endometrium.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEACAM1 antibody [EPR26490-88] (BSA and Azide free) (AB300062)This data was developed using ab300061, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human endometrium tissue labelling CEACAM1 with ab300061 at 1/2000 followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). Positive staining on human endometrium (PMID: 9626043). The section was incubated with ab300061 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: PBS was used instead of primary antibody (ab300061) followed by ready to use secondary antibody LeicaDS9800 (BOND™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEACAM1 antibody [EPR26490-88] (BSA and Azide free) (AB300062)](https://www.abcam.co.jp/ps/products/300/ab300062/Images/AB300062-4-anti-ceacam1-antibody-epr2649088-immunohistochemistry-human-liver.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEACAM1 antibody [EPR26490-88] (BSA and Azide free) (AB300062)This data was developed using ab300061, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human liver tissue labelling CEACAM1 with ab300061 at 1/2000 followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). Positive staining on human liver (PMID: 27377843). The section was incubated with ab300061 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: PBS was used instead of primary antibody (ab300061) followed by ready to use secondary antibody LeicaDS9800 (BOND™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEACAM1 antibody [EPR26490-88] (BSA and Azide free) (AB300062)](https://www.abcam.co.jp/ps/products/300/ab300062/Images/AB300062-5-anti-ceacam1-antibody-epr2649088-immunohistochemistry-human-hepatic-carcin.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEACAM1 antibody [EPR26490-88] (BSA and Azide free) (AB300062)This data was developed using ab300061, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human hepatic carcinoma tissue labelling CEACAM1 with ab300061 at 1/2000 followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). Positive staining on human hepatic carcinoma (PMID: 27377843). The section was incubated with ab300061 for 30 mins at room temperature The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: PBS was used instead of primary antibody (ab300061) followed by ready to use secondary antibody LeicaDS9800 (BOND™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
-
![Immunocytochemistry/ Immunofluorescence - Anti-CEACAM1 antibody [EPR26490-88] (BSA and Azide free) (ab300062)](https://www.abcam.co.jp/ps/products/300/ab300062/Images/AB300062-6-anti-ceacam1-antibody-epr2649088-immunocytochemistry-ceacam1-ko-a549.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CEACAM1 antibody [EPR26490-88] (BSA and Azide free) (ab300062)This data was developed using ab300061, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeilized CEACAM1 KO A549 (CEACAM1 knockout human lung carcinoma epithelial cell) (ab267047) cells lebelling CEACAM1 with ab300061 at 1/500 dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green). Confocal image showing membrane and cytoplasmic staining in parental A549 cells, no staining in CEACAM1 KO A549 cells.
ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.
-
![Flow Cytometry - Anti-CEACAM1 antibody [EPR26490-88] (BSA and Azide free) (AB300062)](https://www.abcam.co.jp/ps/products/300/ab300062/Images/AB300062-7-anti-ceacam1-antibody-epr2649088-flow-cytometry-wildtype-a549.jpg)
Flow Cytometry - Anti-CEACAM1 antibody [EPR26490-88] (BSA and Azide free) (AB300062)This data was developed using ab300061, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of Wild-type A549 (Human lung carcinoma epithelial cell, Right)/ CEACAM1 knockout A549 cells (Left) cells labelling CEACAM1 with ab300061 at 1/500 dilution/ Left and Right compared with a isotype control. Goat anti-Rabbit IgG (Alexa Fluor® 647, ab150083) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells. Positive staining on A549 cells (ab255450), while no staining on CEACAM1 knockout A549 cells (ab267047).
-
![Immunoprecipitation - Anti-CEACAM1 antibody [EPR26490-88] (BSA and Azide free) (AB300062)](https://www.abcam.co.jp/ps/products/300/ab300062/Images/AB300062-8-anti-ceacam1-antibody-epr2649088-immunoprecipitation-a549.jpg)
Immunoprecipitation - Anti-CEACAM1 antibody [EPR26490-88] (BSA and Azide free) (AB300062)This data was developed using ab300061, the same antibody clone in a different buffer formulation.
CEACAM1 was immunoprecipitated from 0.35 mg A549 (human lung carcinoma epithelial cell) whole cell lysate 10 µg with ab300061 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab300061 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.Lane 1: A549 (human lung carcinoma epithelial cell) whole cell lysate 10 µg
Lane 2: abab300061 IP in A549 whole cell lysate
Lane 3:Rabbit monoclonal IgG (ab172730) instead of ab300061 in A549 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 32 seconds
-
![Anti-CEACAM1 antibody [EPR26490-88] (BSA and Azide free) (AB300062)](https://www.abcam.co.jp/ps/products/300/ab300062/Images/AB300062-1-Benefits-of-Recombinant-antibodies.jpg)
Anti-CEACAM1 antibody [EPR26490-88] (BSA and Azide free) (AB300062)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
-
Datasheet download
Certificate of Compliance
参考文献 (0)
ab300062 は論文での使用が確認できていません。
