Anti-CDYL 抗体 (ab5188)
Key features and details
- Rabbit polyclonal to CDYL
- Suitable for: IHC-P, ChIP, WB, ChIP-sequencing
- Reacts with: Mouse, Rat, Human, African green monkey
- Isotype: IgG
製品の概要
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製品名
Anti-CDYL antibody
CDYL 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to CDYL -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, ChIP, WB, ChIP-sequencingmore details -
種交差性
交差種: Mouse, Rat, Human, African green monkey -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- This antibody gave a positive signal in COS7 cells transfected with Human CDYL (Whole Cell Lysate)
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab5188の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
Use at an assay dependent concentration.
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ChIP | (1) |
Use at an assay dependent concentration. PubMed: 22009739
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WB | (1) |
1/1000. Detects a band of approximately 80 kDa (predicted molecular weight: 66 kDa).
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ChIP-sequencing |
Use at an assay dependent concentration. PubMed: 22009739
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特記事項 |
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IHC-P
Use at an assay dependent concentration. |
ChIP
Use at an assay dependent concentration. PubMed: 22009739 |
WB
1/1000. Detects a band of approximately 80 kDa (predicted molecular weight: 66 kDa). |
ChIP-sequencing
Use at an assay dependent concentration. PubMed: 22009739 |
ターゲット情報
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機能
Acts as repressor of transcription (By similarity). Has histone acetyltransferase activity, with a preference for histone H4. -
組織特異性
Ubiquitous. Expressed at moderate levels in all tissues examined. -
配列類似性
Contains 1 chromo domain. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 9425 Human
- Entrez Gene: 12593 Mouse
- Entrez Gene: 361237 Rat
- Omim: 603778 Human
- SwissProt: Q9Y232 Human
- SwissProt: Q9WTK2 Mouse
- SwissProt: Q6AYK9 Rat
- Unigene: 269092 Human
see all -
別名
- CDY-like antibody
- CDY-like autosomal antibody
- Cdyl antibody
see all
画像
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All lanes : Anti-CDYL antibody (ab5188) at 1 µg/ml
Lane 1 : COS-7 (African green monkey kidney fibroblast-like cell line) whole cell lysate - Untransfected
Lane 2 : COS7 CDYL (Human) Transfected Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 66 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted?
Additional bands at: 60 kDa, 65 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 12 minutes -
All lanes : Anti-CDYL antibody (ab5188) at 1/1000 dilution
Lane 1 : COS-7 (African green monkey kidney fibroblast-like cell line) whole cell lysate
Lane 2 : COS7 cells transfected with HA-CDYL-FLAG plasmid
Predicted band size: 66 kDa
Observed band size: 83 kDa why is the actual band size different from the predicted?Rabbit polyclonal to CDYL (ab5188) at 1/1000.
Left lane (-) COS7 (monkey) cells.
Right lane (+) COS7 cells transfected with HA-CDYL-FLAG plasmid.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (13)
ab5188 は 13 報の論文で使用されています。
- Tian H et al. EWSR1 affects PRDM9-dependent histone 3 methylation and provides a link between recombination hotspots and the chromosome axis protein REC8. Mol Biol Cell 32:1-14 (2021). PubMed: 33175657
- Pham V et al. Proteomic Analyses Identify a Novel Role for EZH2 in the Initiation of Cancer Cell Drug Tolerance. J Proteome Res 19:1533-1547 (2020). PubMed: 32159963
- Qiu Z et al. CDYL promotes the chemoresistance of small cell lung cancer by regulating H3K27 trimethylation at the CDKN1C promoter. Theranostics 9:4717-4729 (2019). PubMed: 31367252
- Wang J et al. Asymmetric Expression of LincGET Biases Cell Fate in Two-Cell Mouse Embryos. Cell 175:1887-1901.e18 (2018). PubMed: 30550787
- Venkatesan N et al. EZH2 promotes neoplastic transformation through VAV interaction-dependent extranuclear mechanisms. Oncogene 37:461-477 (2018). PubMed: 28967906