Anti-CDKN2A/p19ARF 抗体 [5-C3] - BSA and Azide free (ab174939)
Key features and details
- Rat monoclonal [5-C3] to CDKN2A/p19ARF - BSA and Azide free
- Suitable for: WB, IP, IHC-P, ICC/IF
- Reacts with: Mouse
- Isotype: IgG2b
Related conjugates and formulations
製品の概要
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製品名
Anti-CDKN2A/p19ARF antibody [5-C3] - BSA and Azide free
CDKN2A/p19ARF 一次抗体 製品一覧 -
製品の詳細
Rat monoclonal [5-C3] to CDKN2A/p19ARF - BSA and Azide free -
由来種
Rat -
アプリケーション
適用あり: WB, IP, IHC-P, ICC/IFmore details -
種交差性
交差種: Mouse
非交差種: Hamster, Human -
免疫原
Synthetic peptide corresponding to Mouse CDKN2A/p19ARF conjugated to keyhole limpet haemocyanin.
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ポジティブ・コントロール
- 3T3 cell lystate positive control.
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特記事項
ab174939 is the carrier-free version of ab26696.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Constituent: PBS -
キャリア・フリー
はい -
Concentration information loading...
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精製度
IgG fraction -
ポリ/モノ
モノクローナル -
クローン名
5-C3 -
ミエローマ
Y3/Ag1.2.3 -
アイソタイプ
IgG2b -
研究分野
関連製品
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Alternative Versions
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Conjugation kits
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab174939の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 17 kDa.
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IP |
Use at an assay dependent concentration.
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IHC-P |
Use a concentration of 5 µg/ml.
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ICC/IF |
Use at an assay dependent concentration.
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特記事項 |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 17 kDa. |
IP
Use at an assay dependent concentration. |
IHC-P
Use a concentration of 5 µg/ml. |
ICC/IF
Use at an assay dependent concentration. |
ターゲット情報
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関連性
The gene for CDK2NA generates several transcripts/proteins which differ from each other in their first exons. Three of these transcripts are generated by alternative splicing (isoform 1 a.k.a p16INK4A, isoform 2 and isoform 3 a.k.a p12), two of which are known to function as inhibitors of CDK4 kinase. One other transcript that is generated from this gene contains an alternate reading frame (ARF), with the first exon located 20kb upstream of the remainder of the gene(isoform 4 a.k.a. p14ARF, p19ARF, ARF). In spite of the structural and some functional differences, all the proteins encoded by the CDKN2A gene are involved in cell cycle G1 control. -
細胞内局在
Nuclear -
参照データベース
- Entrez Gene: 12578 Mouse
- SwissProt: Q64364 Mouse
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別名
- ARF antibody
- CDKN2A antibody
- Cyclin dependent kinase inhibitor 2A antibody
see all
画像
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Immunocytochemistry/ Immunofluorescence - Anti-CDKN2A/p19ARF antibody [5-C3] - BSA and Azide free (ab174939)
This ICC/IF data was generated using the same antibody clone in a different buffer formulation (ab26696).
Immunofluorescent detection of p19ARF. MEF cells (1 and 2) or NIH3T3 cells with arf gene deleted (3 and 4) were stained with anti-p19ARF 5C3 followed by a fluorescent-labelled anti-rat IgG (1 and 3) and Hoechst dye to visualise nuclei (2 and 4).
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (0)
ab174939 は論文での使用が確認できていません。