Anti-CDKN2A/p19ARF 抗体 [5-C3] (ab26696)
![Immunocytochemistry/ Immunofluorescence - Anti-CDKN2A/p19ARF antibody [5-C3] (ab26696)](https://www.abcam.co.jp/ps/products/26/ab26696/Images/ab26696-482439-AntiCDKN2A-p19ARF-antibody-immunocytochemistry-mef1.jpg "Immunocytochemistry/ Immunofluorescence - Anti-CDKN2A/p19ARF antibody [5-C3] (ab26696)")
Key features and details
- Rat monoclonal [5-C3] to CDKN2A/p19ARF
- Suitable for: WB, IP, ICC/IF, IHC-FoFr
- Reacts with: Mouse
- Isotype: IgG2b
Related conjugates and formulations
製品の概要
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製品名
Anti-CDKN2A/p19ARF antibody [5-C3]
CDKN2A/p19ARF 一次抗体 製品一覧 -
製品の詳細
Rat monoclonal [5-C3] to CDKN2A/p19ARF -
由来種
Rat -
アプリケーション
適用あり: WB, IP, ICC/IF, IHC-FoFrmore details -
種交差性
交差種: Mouse
非交差種: Hamster, Human -
免疫原
Synthetic peptide corresponding to Mouse CDKN2A/p19ARF conjugated to keyhole limpet haemocyanin.
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ポジティブ･コントロール
- ICC/IF: MEF1 cells WB: MEF1 cell lysate, NIH3T3 cells
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特記事項
The 5-C3 monoclonal has been knockout validated in WB and ICC/IF using wild type and knockout mouse samples. The data are shown in the images section.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.50
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine -
Concentration information loading... -
精製度
Protein G purified -
ポリ/モノ
モノクローナル -
クローン名
5-C3 -
ミエローマ
Y3/Ag1.2.3 -
アイソタイプ
IgG2b -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab26696の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 19 kDa (predicted molecular weight: 17 kDa).
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| IP |
Use at an assay dependent concentration.
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| ICC/IF |
1/1000.
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| IHC-FoFr |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 19 kDa (predicted molecular weight: 17 kDa). |
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IP
Use at an assay dependent concentration. |
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ICC/IF
1/1000. |
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IHC-FoFr
Use at an assay dependent concentration. |
ターゲット情報
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関連性
The gene for CDK2NA generates several transcripts/proteins which differ from each other in their first exons. Three of these transcripts are generated by alternative splicing (isoform 1 a.k.a p16INK4A, isoform 2 and isoform 3 a.k.a p12), two of which are known to function as inhibitors of CDK4 kinase. One other transcript that is generated from this gene contains an alternate reading frame (ARF), with the first exon located 20kb upstream of the remainder of the gene(isoform 4 a.k.a. p14ARF, p19ARF, ARF). In spite of the structural and some functional differences, all the proteins encoded by the CDKN2A gene are involved in cell cycle G1 control. -
細胞内局在
Nuclear -
参照データベース
- Entrez Gene: 12578 Mouse
- SwissProt: Q64364 Mouse
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別名
- ARF antibody
- CDKN2A antibody
- Cyclin dependent kinase inhibitor 2A antibody
see all
画像
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Immunocytochemistry/ Immunofluorescence - Anti-CDKN2A/p19ARF antibody [5-C3] (ab26696)
ab26696 staining CDKN2A/p19ARF in MEF1 cells. The cells were fixed with 4% PFA (10 mins), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab26696 at 5µg/ml and ab190573, Rabbit monoclonal to alpha Tubulin (Alexa Fluor® 647), at 2µg/ml (shown in red). The secondary antibody (shown in green) was ab150165, Alexa Fluor® 488 Goat anti-Rat IgG (H+L) used at a 1/1000 dilution for 1h at room temperature. Nuclear DNA was labelled with DAPI (shown in blue). Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
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![Immunocytochemistry/ Immunofluorescence - Anti-CDKN2A/p19ARF antibody [5-C3] (ab26696)](https://www.abcam.co.jp/ps/products/26/ab26696/Images/ab26696-37662-anti-cdkn2a-p19arf-antibody-5-c3-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CDKN2A/p19ARF antibody [5-C3] (ab26696)Immunofluorescent detection of p19ARF. MEF cells (1 and 2) or NIH3T3 cells with arf gene deleted (3 and 4) were stained with anti-p19ARF 5C3 followed by a fluorescent-labelled anti-rat IgG (1 and 3) and Hoechst dye to visualise nuclei (2 and 4). -
![Western blot - Anti-CDKN2A/p19ARF antibody [5-C3] (ab26696)](https://www.abcam.co.jp/ps/products/26/ab26696/Images/ab26696-482446-anti-cdkn2a-antibody-western-blot.jpg)
Western blot - Anti-CDKN2A/p19ARF antibody [5-C3] (ab26696)Anti-CDKN2A/p19ARF antibody [5-C3] (ab26696) at 2 µg/ml + MEF-1 whole cell lysate at 20 µg
Secondary
Goat polyclonal to Rat IgG - H&L - Pre-Adsorbed (HRP) at 1/10000 dilution
Predicted band size: 17 kDa
Observed band size: 19,85 kDa why is the actual band size different from the predicted?
Exposure time: 1 minuteBlocking buffer: 2% BSA
Gel type: MES
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![Western blot - Anti-CDKN2A/p19ARF antibody [5-C3] (ab26696)](https://www.abcam.co.jp/ps/products/26/ab26696/Images/ab26696-37664-anti-cdkn2a-p19arf-antibody-5-c3-western-blot.jpg)
Western blot - Anti-CDKN2A/p19ARF antibody [5-C3] (ab26696)All lanes : Anti-CDKN2A/p19ARF antibody [5-C3] (ab26696)
Lane 1 : Total cell lysate from NIH3T3 cells with Arf gene deleted
Lane 2 : MEF total cell lysate
Secondary
All lanes : anti-rat IgG-HRP
Predicted band size: 17 kDa
Observed band size: 19 kDa why is the actual band size different from the predicted? -
![Immunoprecipitation - Anti-CDKN2A/p19ARF antibody [5-C3] (ab26696)](https://www.abcam.co.jp/ps/products/26/ab26696/Images/ab26696-37663-anti-cdkn2a-p19arf-antibody-5-c3-immunoprecipitation.jpg)
Immunoprecipitation - Anti-CDKN2A/p19ARF antibody [5-C3] (ab26696)Immunoprecipitation using anti-p19ARF 5C3. NIH3T3 cells expressing HA-p19ARF were incubated with a control rat IgG (lane 1) or anti-p19ARF 5C3 (2). Precipitated proteins were analysed by Western blot using anti-HA antibody.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (22)
ab26696 は 22 報の論文で使用されています。
- Zhu M et al. AMPK Activator O304 Protects Against Kidney Aging Through Promoting Energy Metabolism and Autophagy. Front Pharmacol 13:836496 (2022). PubMed: 35308246
- Zhu M et al. KYA1797K, a Novel Small Molecule Destabilizing β-Catenin, Is Superior to ICG-001 in Protecting against Kidney Aging. Kidney Dis (Basel) 8:408-423 (2022). PubMed: 36466073
- Strauss RP et al. Loss of ARF/INK4A Promotes Liver Progenitor Cell Transformation Toward Tumorigenicity Supporting Their Role in Hepatocarcinogenesis. Gene Expr 20:39-52 (2020). PubMed: 32317048
- Sun L et al. Nicotine triggers islet ß cell senescence to facilitate the progression of type 2 diabetes. Toxicology 441:152502 (2020). PubMed: 32473187
- Chu B et al. ALOX12 is required for p53-mediated tumour suppression through a distinct ferroptosis pathway. Nat Cell Biol 21:579-591 (2019). PubMed: 30962574