Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
リコンビナントRabMAb

Anti-CDKN2A/p16INK4A + CDKN2B/p15INK4B 抗体 [EP435Y] - BSA and Azide free (ab247292)

Submit a review Submit a question
Flow Cytometry (Intracellular) - Anti-CDKN2A/p16INK4A + CDKN2B/p15INK4B antibody [EP435Y] - BSA and Azide free (ab247292)
  • Western blot - Anti-CDKN2A/p16INK4A + CDKN2B/p15INK4B antibody [EP435Y] - BSA and Azide free (ab247292)
  • Western blot - Anti-CDKN2A/p16INK4A + CDKN2B/p15INK4B antibody [EP435Y] - BSA and Azide free (ab247292)
  • Western blot - Anti-CDKN2A/p16INK4A + CDKN2B/p15INK4B antibody [EP435Y] - BSA and Azide free (ab247292)
  • Immunoprecipitation - Anti-CDKN2A/p16INK4A + CDKN2B/p15INK4B antibody [EP435Y] - BSA and Azide free (ab247292)
  • Anti-CDKN2A/p16INK4A + CDKN2B/p15INK4B antibody [EP435Y] - BSA and Azide free (ab247292)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP435Y] to CDKN2A/p16INK4A +CDKN2B/p15INK4B - BSA and Azide free
  • Suitable for: WB, Flow Cyt (Intra), IP
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Unconjugated

製品の概要

  • 製品名

    Anti-CDKN2A/p16INK4A + CDKN2B/p15INK4B antibody [EP435Y] - BSA and Azide free
    CDKN2A/p16INK4A +CDKN2B/p15INK4B 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EP435Y] to CDKN2A/p16INK4A +CDKN2B/p15INK4B - BSA and Azide free

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, Flow Cyt (Intra), IPmore details
    適用なし: ICC/IF or IHC

  • 種交差性

    交差種: Human

  • 免疫原

    Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: Human CDKN2A full-length recombinant protein with His-tag, Human CDKN2B full-length recombinant protein with GST-tag, HEK 293, HeLa Flow Cyt (intra): HEK-293 cells IP: HeLa whole cell lysate.

  • 特記事項

    ab247292 is the carrier-free version of ab40803.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab247292の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
Use at an assay dependent concentration. Detects a band of approximately 17 kDa (predicted molecular weight: 17 kDa).
Flow Cyt (Intra)
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
特記事項
WB
Use at an assay dependent concentration. Detects a band of approximately 17 kDa (predicted molecular weight: 17 kDa).
Flow Cyt (Intra)
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
追加情報
Is unsuitable for ICC/IF or IHC.

ターゲット情報

  • 機能

    Acts as a negative regulator of the proliferation of normal cells by interacting strongly with CDK4 and CDK6. This inhibits their ability to interact with cyclins D and to phosphorylate the retinoblastoma protein.

  • 組織特異性

    Widely expressed but not detected in brain or skeletal muscle. Isoform 3 is pancreas-specific.

  • 関連疾患

    The association between cutaneous and uveal melanomas in some families suggests that mutations in CDKN2A may account for a proportion of uveal melanomas. However, CDKN2A mutations are rarely found in uveal melanoma patients.
    Melanoma, cutaneous malignant 2
    Familial atypical multiple mole melanoma-pancreatic carcinoma syndrome
    Melanoma-astrocytoma syndrome

  • 配列類似性

    Belongs to the CDKN2 cyclin-dependent kinase inhibitor family.
    Contains 4 ANK repeats.

  • 翻訳後修飾

    Phosphorylation seems to increase interaction with CDK4.

  • 細胞内局在

    Cytoplasm. Nucleus.
  • Information by UniProt

  • 参照データベース

    • 別名

      • CDK4I antibody
      • CDKN2A antibody
      • CDN2A_HUMAN antibody
      • Cyclin-dependent kinase 4 inhibitor A antibody
      • Cyclin-dependent kinase inhibitor 2A antibody
      • MTS-1 antibody
      • Multiple tumor suppressor 1 antibody
      • p16-INK4 antibody
      • p16-INK4a antibody
      • p16INK4A antibody
      see all

    画像

    • Flow Cytometry (Intracellular) - Anti-CDKN2A/p16INK4A + CDKN2B/p15INK4B antibody [EP435Y] - BSA and Azide free (ab247292)
      Flow Cytometry (Intracellular) - Anti-CDKN2A/p16INK4A + CDKN2B/p15INK4B antibody [EP435Y] - BSA and Azide free (ab247292)

      This data was developed using ab40803, the same antibody clone in a different buffer formulation.

      Intracellular Flow Cytometry analysis of HEK-293 (Human embryonic kidney epithelial cell) cells labeling CDKN2A/p16INK4a with purified ab40803 at 1/100 dilution (10 µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cells without incubation with primary antibody and secondary antibody (Blue).

    • Western blot - Anti-CDKN2A/p16INK4A + CDKN2B/p15INK4B antibody [EP435Y] - BSA and Azide free (ab247292)
      Western blot - Anti-CDKN2A/p16INK4A + CDKN2B/p15INK4B antibody [EP435Y] - BSA and Azide free (ab247292)
      Anti-CDKN2A/p16INK4A + CDKN2B/p15INK4B antibody [EP435Y] (ab40803) at 1/1000 dilution + 293 cell lysate at 10 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051)

      Predicted band size: 17 kDa
      Observed band size: 17 kDa



      This data was developed using ab40803, the same antibody clone in a different buffer formulation.

    • Western blot - Anti-CDKN2A/p16INK4A + CDKN2B/p15INK4B antibody [EP435Y] - BSA and Azide free (ab247292)
      Western blot - Anti-CDKN2A/p16INK4A + CDKN2B/p15INK4B antibody [EP435Y] - BSA and Azide free (ab247292)
      All lanes : Anti-CDKN2A/p16INK4A + CDKN2B/p15INK4B antibody [EP435Y] (ab40803) at 1/1000 dilution

      Lane 1 : HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate
      Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate

      Lysates/proteins at 15 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 17 kDa
      Observed band size: 17 kDa



      This data was developed using ab40803, the same antibody clone in a different buffer formulation. 

      5% NFDM/TBST was used as a blocking and diluting buffer. 

      Exposure times: 

      Lane 1: 20 seconds

      Lane 2: 180 seconds

    • Western blot - Anti-CDKN2A/p16INK4A + CDKN2B/p15INK4B antibody [EP435Y] - BSA and Azide free (ab247292)
      Western blot - Anti-CDKN2A/p16INK4A + CDKN2B/p15INK4B antibody [EP435Y] - BSA and Azide free (ab247292)
      All lanes : Anti-CDKN2A/p16INK4A + CDKN2B/p15INK4B antibody [EP435Y] (ab40803) at 1/10000 dilution

      Lane 1 : Human CDKN2A full-length recombinant protein with His-tag
      Lane 2 : Human CDKN2B full-length recombinant protein with GST-tag

      Lysates/proteins at 0.01 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 17 kDa
      Observed band size: 17, 40 kDa why is the actual band size different from the predicted?


      Exposure time: 10 seconds


      This data was developed using ab40803, the same antibody clone in a different buffer formulation.

      5% NFDM/TBST was used as a blocking and diluting buffer. 

    • Immunoprecipitation - Anti-CDKN2A/p16INK4A + CDKN2B/p15INK4B antibody [EP435Y] - BSA and Azide free (ab247292)
      Immunoprecipitation - Anti-CDKN2A/p16INK4A + CDKN2B/p15INK4B antibody [EP435Y] - BSA and Azide free (ab247292)

      This data was developed using ab40803, the same antibody clone in a different buffer formulation.

      Purified ab40803 at 1/50 dilution (2µg) immunoprecipitating CDKN2A/p16INK4a in HeLa whole cell lysate.
      Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10µg
      Lane 2 (+): ab40803 + HeLa whole cell lysate.
      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab40803 in HeLa whole cell lysate.
      VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
      Blocking Buffer and concentration: 5% NFDM/TBST.
      Diluting buffer and concentration: 5% NFDM/TBST.
      Observed band size: 17 kDa

    • Anti-CDKN2A/p16INK4A + CDKN2B/p15INK4B antibody [EP435Y] - BSA and Azide free (ab247292)
      Anti-CDKN2A/p16INK4A + CDKN2B/p15INK4B antibody [EP435Y] - BSA and Azide free (ab247292)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab247292 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab247292 は論文での使用が確認できていません。

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    There are currently no Customer reviews or Questions for ab247292.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com