Anti-CDKN2A/p16INK4A + CDKN2B/p15INK4B 抗体 [EP435Y-129R] (ab81278)
![Flow Cytometry (Intracellular) - Anti-CDKN2A/p16INK4A + CDKN2B/p15INK4B antibody [EP435Y-129R] (ab81278)](https://www.abcam.co.jp/ps/products/81/ab81278/Images/ab81278-483458-anti-cdkn2a-p16ink4a-cdkn2b-p15ink4b-antibody-ep435y-129r-flow-cytometry-hek293-human.jpg "Flow Cytometry (Intracellular) - Anti-CDKN2A/p16INK4A + CDKN2B/p15INK4B antibody [EP435Y-129R] (ab81278)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP435Y-129R] to CDKN2A/p16INK4A +CDKN2B/p15INK4B
- Suitable for: Flow Cyt (Intra), WB, ICC/IF, IP
- Reacts with: Human
Related conjugates and formulations
製品の概要
-
製品名
Anti-CDKN2A/p16INK4A + CDKN2B/p15INK4B antibody [EP435Y-129R]
CDKN2A/p16INK4A +CDKN2B/p15INK4B 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EP435Y-129R] to CDKN2A/p16INK4A +CDKN2B/p15INK4B -
由来種
Rabbit -
アプリケーション
適用あり: Flow Cyt (Intra), WB, ICC/IF, IPmore details
適用なし: IHC-P -
種交差性
交差種: Human -
免疫原
Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
-
ポジティブ･コントロール
- HeLa cells and 293 cell lysate WB: Human CDKN2A full-length recombinant protein with His-tag, Human CDKN2B full-length recombinant protein with GST-tag
-
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EP435Y-129R -
アイソタイプ
IgG -
研究分野
関連製品
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab81278の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt (Intra) |
1/50 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
| WB |
1/2000. Predicted molecular weight: 17 kDa.
|
|
| ICC/IF |
1/100 - 1/500.
|
|
| IP |
Use at an assay dependent concentration.
|
| 特記事項 |
|---|
|
Flow Cyt (Intra)
1/50 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB
1/2000. Predicted molecular weight: 17 kDa. |
|
ICC/IF
1/100 - 1/500. |
|
IP
Use at an assay dependent concentration. |
ターゲット情報
-
機能
Acts as a negative regulator of the proliferation of normal cells by interacting strongly with CDK4 and CDK6. This inhibits their ability to interact with cyclins D and to phosphorylate the retinoblastoma protein. -
組織特異性
Widely expressed but not detected in brain or skeletal muscle. Isoform 3 is pancreas-specific. -
関連疾患
The association between cutaneous and uveal melanomas in some families suggests that mutations in CDKN2A may account for a proportion of uveal melanomas. However, CDKN2A mutations are rarely found in uveal melanoma patients.
Melanoma, cutaneous malignant 2
Familial atypical multiple mole melanoma-pancreatic carcinoma syndrome
Melanoma-astrocytoma syndrome -
配列類似性
Belongs to the CDKN2 cyclin-dependent kinase inhibitor family.
Contains 4 ANK repeats. -
翻訳後修飾
Phosphorylation seems to increase interaction with CDK4. -
細胞内局在
Cytoplasm. Nucleus. - Information by UniProt
-
参照データベース
- Entrez Gene: 1029 Human
- SwissProt: P42771 Human
- Unigene: 512599 Human
-
別名
- CDK4I antibody
- CDKN2A antibody
- CDN2A_HUMAN antibody
see all
画像
-
Flow Cytometry (Intracellular) - Anti-CDKN2A/p16INK4A + CDKN2B/p15INK4B antibody [EP435Y-129R] (ab81278)
Flow cytometric analysis of 4% paraformaldehyde fixed and 90% methanol permeabilised HEK-293 (Human embryonic kidney epithelial cell) with ab81278 at 1/20 dilution (red) compared with a Rabbit monoclonal IgG (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.
-
![Immunocytochemistry/ Immunofluorescence - Anti-CDKN2A/p16INK4A +CDKN2B/p15INK4B antibody [EP435Y-129R] (ab81278)](https://www.abcam.co.jp/ps/products/81/ab81278/Images/ab81278-483457-anti-cdkn2a-p16ink4a-cdkn2b-p15ink4b-antibody-ep435y-129r-immunocytochemistry-immunofluorescence-hela-human.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CDKN2A/p16INK4A +CDKN2B/p15INK4B antibody [EP435Y-129R] (ab81278)Immunofluorescent analysis of 4% Paraformaldehyde fixed 0.1% Triton X-100 permeabilised HeLa (human cervix adenocarcinoma epithelial cell) labelling CDKN2A/p16INK4a with ab81278 at 1/250 dilution, followed by AlexaFluor® 488 Goat anti-Rabbit secondary antibody (ab150077) at 1/1000 dilution (green). Confocal image showing cytoplasmic and nuclear staining in HeLa is observed. Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) was used to counterstain alpha Tubulin at 1/200 dilution (red). The Nuclear counterstain was DAPI (blue).
Secondary antibody only control: Secondary antibody is AlexaFluor® 488 Goat anti-Rabbit secondary antibody (ab150077) at 1/1000 dilution (green).
-
![Anti-CDKN2A/p16INK4A +CDKN2B/p15INK4B antibody [EP435Y-129R] (ab81278)](https://www.abcam.co.jp/ps/products/81/ab81278/Images/ab81278-482806-Benefits-of-Recombinant-antibodies.jpg)
Anti-CDKN2A/p16INK4A +CDKN2B/p15INK4B antibody [EP435Y-129R] (ab81278) -
![Western blot - Anti-CDKN2A/p16INK4A +CDKN2B/p15INK4B antibody [EP435Y-129R] (ab81278)](https://www.abcam.co.jp/ps/products/81/ab81278/Images/ab81278-482805-WB-81278.jpg)
Western blot - Anti-CDKN2A/p16INK4A +CDKN2B/p15INK4B antibody [EP435Y-129R] (ab81278)All lanes : Anti-CDKN2A/p16INK4A + CDKN2B/p15INK4B antibody [EP435Y-129R] (ab81278) at 1/20000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : 293 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labeled goat anti rabbit at 1/2000 dilution
Predicted band size: 17 kDa
Observed band size: 17 kDa -
![Western blot - Anti-CDKN2A/p16INK4a antibody [EP435Y-129R] (ab81278)](https://www.abcam.co.jp/ps/products/81/ab81278/Images/ab81278-482797-ab81278-WB.jpg)
Western blot - Anti-CDKN2A/p16INK4a antibody [EP435Y-129R] (ab81278)All lanes : Anti-CDKN2A/p16INK4A + CDKN2B/p15INK4B antibody [EP435Y-129R] (ab81278) at 1/1000 dilution
Lane 1 : Human CDKN2A full-length recombinant protein with His-tag
Lane 2 : Human CDKN2B full-length recombinant protein with GST-tag
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 17 kDa
Observed band size: 17, 40 kDa why is the actual band size different from the predicted?
Exposure time: 10 seconds5% NFDM/TBST was used as blocking and iluting buffer.
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (33)
ab81278 は 33 報の論文で使用されています。
- Stanzione M et al. Translesion DNA synthesis mediates acquired resistance to olaparib plus temozolomide in small cell lung cancer. Sci Adv 8:eabn1229 (2022). PubMed: 35559669
- Horn G et al. Sulfur mustard single-dose exposure triggers senescence in primary human dermal fibroblasts. Arch Toxicol 96:3053-3066 (2022). PubMed: 35906424
- Cho HJ et al. Nintedanib induces senolytic effect via STAT3 inhibition. Cell Death Dis 13:760 (2022). PubMed: 36055997
- Li Z et al. P14AS upregulates gene expression in the CDKN2A/2B locus through competitive binding to PcG protein CBX7. Front Cell Dev Biol 10:993525 (2022). PubMed: 36176277
- Chintala S et al. High expression of NFX1-123 in HPV positive head and neck squamous cell carcinomas. Head Neck 44:177-188 (2022). PubMed: 34693597