Anti-CDKL5 抗体 (ab22453)
Key features and details
- Rabbit polyclonal to CDKL5
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
製品の概要
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製品名
Anti-CDKL5 antibody
CDKL5 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to CDKL5 -
由来種
Rabbit -
特異性
Swissprot predicts mouse CDKL5 protein to be ~105 kDa (Q3UTQ8), however a recent paper by Lin et al, 2006 (PMID: 16330482) indicated CDKL5 to run at ~118 kDa on WB. On this basis we think that the ~120 kDa band we observe using Rabbit polyclonal to CDKL5 (ab22453) on mouse tissue is CDKL5 specific. Futhermore the ~120 kDa band is blocked by the immunising peptide. The larger ~200 kDa is potentially also CDKL5 as a similar band is also observed in the Lin et al (2006) publication. There is evidence for larger forms of CDKL5 for the human version of the protein, so it may not be surprising to find a similar scenario for the mouse protein. -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse
交差が予測される動物種: Human
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab22453の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 120,160 kDa (predicted molecular weight: 105 kDa).
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| 特記事項 |
|---|
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WB
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 120,160 kDa (predicted molecular weight: 105 kDa). |
ターゲット情報
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機能
Mediates phosphorylation of MECP2. -
組織特異性
Expressed in brain, lung, kidney, prostate, ovary, placenta, pancreas and testis. -
関連疾患
Note=Chromosomal aberrations involving CDKL5 are found in patients manifesting early-onset seizures and spams and psychomotor impairment. Translocation t(X;6)(p22.3;q14); translocation t(X;7)(p22.3;p15).
Defects in CDKL5 are a cause of epileptic encephalopathy early infantile type 2 (EIEE2) [MIM:300672]; also known as atypical CDKL5-related Rett syndrome. EIEE2 is a severe form of epilepsy characterized by seizures or spasms beginning in infancy. Patients manifest features resembling Rett syndrome such as microcephaly, lack of speech development, stereotypic hand movements. -
配列類似性
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
Contains 1 protein kinase domain. -
翻訳後修飾
Autophosphorylated. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 6792 Human
- Entrez Gene: 382253 Mouse
- Omim: 300203 Human
- SwissProt: O76039 Human
- SwissProt: Q3UTQ8 Mouse
- Unigene: 659851 Human
- Unigene: 443717 Mouse
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別名
- Cdkl5 antibody
- CDKL5_HUMAN antibody
- Cyclin dependent kinase 5 transcript antibody
see all
画像
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Western blot - Anti-CDKL5 antibody (ab22453)This image is courtesy of Chris Anderson, Wellcome Trust Sanger Institute, United KingdomAll lanes : Anti-CDKL5 antibody (ab22453) at 3 µg/ml
Lane 1 : mouse cortex
Lane 2 : mouse cortex with Human CDKL5 peptide (ab25756) at 2 µg/ml
Lysates/proteins at 20 µg per lane.
Predicted band size: 105 kDa
Observed band size: 120,200 kDa why is the actual band size different from the predicted?
Exposure time: 10 minutes
Swissprot predicts mouse CDKL5 protein to be ~105kDa (Q3UTQ8), however a recent paper by Lin et al, 2006 (PMID: 16330482) indicated CDKL5 to run at ~118kDa on WB. On this basis we think that the ~120kDa band we observe using Rabbit polyclonal to CDKL5 (ab22453) on mouse tissue is CDKL5 specific. Futhermore the ~120kDa band is blocked by the immunising peptide. The larger >150kDa is potentially also CDKL5 as a similar band is also observed in the Lin et al (2006) publication. There is evidence for larger forms of CDKL5 for the human version of the protein, so it may not be surprising to find a similar scenario for the mouse protein.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab22453 は 5 報の論文で使用されています。
- Clemm von Hohenberg K et al. Cyclin B/CDK1 and Cyclin A/CDK2 phosphorylate DENR to promote mitotic protein translation and faithful cell division. Nat Commun 13:668 (2022). PubMed: 35115540
- Kim JY et al. A kinome-wide screen identifies a CDKL5-SOX9 regulatory axis in epithelial cell death and kidney injury. Nat Commun 11:1924 (2020). PubMed: 32317630
- Baltussen LL et al. Chemical genetic identification of CDKL5 substrates reveals its role in neuronal microtubule dynamics. EMBO J 37:N/A (2018). PubMed: 30266824
- Wang IT et al. Loss of CDKL5 disrupts kinome profile and event-related potentials leading to autistic-like phenotypes in mice. Proc Natl Acad Sci U S A : (2012). WB . PubMed: 23236174
- Nemos C et al. Mutational spectrum of CDKL5 in early-onset encephalopathies: a study of a large collection of French patients and review of the literature. Clin Genet 76:357-71 (2009). WB, ICC/IF ; Human . PubMed: 19793311