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リコンビナントRabMAb

Anti-CDKAL1 抗体 [EPR11621] (ab168390)

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Western blot - Anti-CDKAL1 antibody [EPR11621] (ab168390)
  • Immunoprecipitation - Anti-CDKAL1 antibody [EPR11621] (ab168390)
  • Anti-CDKAL1 antibody [EPR11621] (ab168390)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11621] to CDKAL1
  • Suitable for: WB, IP
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-CDKAL1 antibody [EPR11621]
    CDKAL1 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR11621] to CDKAL1

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IPmore details
    適用なし: Flow Cyt or IHC-P

  • 種交差性

    交差種: Human
    交差が予測される動物種: Mouse, Rat

  • 免疫原

    Recombinant fragment within Human CDKAL1 aa 1-200. The exact sequence is proprietary.
    Database link: Q5VV42

  • ポジティブ･コントロール

    • HepG2, Raji, MDA-MB-435 and HeLa cell lysates.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.

  • バッファー

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • 精製度

    Tissue culture supernatant

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR11621

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab168390の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/1000 - 1/5000. Predicted molecular weight: 65 kDa.
IP
1/10 - 1/100.
特記事項
WB
1/1000 - 1/5000. Predicted molecular weight: 65 kDa.
IP
1/10 - 1/100.
追加情報
Is unsuitable for Flow Cyt or IHC-P.

ターゲット情報

  • 機能

    Catalyzes the methylthiolation of N6-threonylcarbamoyladenosine (t(6)A), leading to the formation of 2-methylthio-N6-threonylcarbamoyladenosine (ms(2)t(6)A) at position 37 in tRNAs that read codons beginning with adenine.

  • 組織特異性

    Expressed in pancreatic islets.

  • 関連疾患

    Genetic variations in CDKAL1 are a cause of susceptibility to diabetes mellitus, non-insulin-dependent (NIDDM) [MIM:125853]. A multifactorial disorder of glucose homeostasis caused by a lack of sensitivity to the body's own insulin. Affected individuals usually have an obese body habitus and manifestations of a metabolic syndrome characterized by diabetes, insulin resistance, hypertension and hypertriglyceridemia. The disease results in long-term complications that affect the eyes, kidneys, nerves, and blood vessels.

  • 配列類似性

    Belongs to the methylthiotransferase family. CDKAL1 subfamily.
    Contains 1 MTTase N-terminal domain.
    Contains 1 TRAM domain.

  • 細胞内局在

    Endoplasmic reticulum membrane. Is a tail-anchored protein that exploits the TCR40 pathway for insertion into the endoplasmic reticulum.
  • Information by UniProt

  • 参照データベース

    • 別名

      • CDK5 regulatory subunit associated protein 1 like 1 antibody
      • CDK5 regulatory subunit-associated protein 1-like 1 antibody
      • CDKAL_HUMAN antibody
      • CDKAL1 antibody
      • Threonylcarbamoyladenosine tRNA methylthiotransferase antibody
      • tRNA t 6 A37 methylthiotransferase antibody
      • tRNA-t(6)A37 methylthiotransferase antibody
      see all

    画像

    • Western blot - Anti-CDKAL1 antibody [EPR11621] (ab168390)
      Western blot - Anti-CDKAL1 antibody [EPR11621] (ab168390)
      All lanes : Anti-CDKAL1 antibody [EPR11621] (ab168390) at 1/1000 dilution

      Lane 1 : HepG2 cell lysate
      Lane 2 : Raji cell lysate
      Lane 3 : MDA-MB-435 cell lysate
      Lane 4 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 65 kDa

    • Immunoprecipitation - Anti-CDKAL1 antibody [EPR11621] (ab168390)
      Immunoprecipitation - Anti-CDKAL1 antibody [EPR11621] (ab168390)
      Detection of CDKAL1 by Western Blot of Immunprecipitate. HepG2 cell lysate immunoprecipitated using ab168390 at 1/10 dilution; HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
    • Anti-CDKAL1 antibody [EPR11621] (ab168390)
      Anti-CDKAL1 antibody [EPR11621] (ab168390)

    参考文献 (0)

    ab168390 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab168390 は論文での使用が確認できていません。

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