Anti-Cdk9 (phospho T186) 抗体 (ab79178)
Key features and details
- Rabbit polyclonal to Cdk9 (phospho T186)
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
製品の概要
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製品名
Anti-Cdk9 (phospho T186) antibody
Cdk9 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Cdk9 (phospho T186) -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse
交差が予測される動物種: Rat
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免疫原
Synthetic peptide corresponding to Human Cdk9 (phospho T186).
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab79178の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/1000. Predicted molecular weight: 43 kDa.
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| 特記事項 |
|---|
|
WB
1/500 - 1/1000. Predicted molecular weight: 43 kDa. |
ターゲット情報
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機能
Member of the cyclin-dependent kinase pair (CDK9/cyclin-T) complex, also called positive transcription elongation factor b (P-TEFb), which facilitates the transition from abortive to production elongation by phosphorylating the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAP II), SUPT5H and RDBP. The CDK9/cyclin-K complex has also a kinase activity toward CTD of RNAP II and can substitute for P-TEFb in vitro. -
組織特異性
Ubiquitous. -
配列類似性
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
Contains 1 protein kinase domain. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 107951 Mouse
- Entrez Gene: 362110 Rat
- SwissProt: Q99J95 Mouse
- SwissProt: Q641Z4 Rat
- Unigene: 27557 Mouse
- Unigene: 98228 Rat
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別名
- C-2K antibody
- CDC2 related kinase antibody
- CDC2L4 antibody
see all
画像
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Western blot - Anti-Cdk9 (phospho T186) antibody (ab79178)All lanes : Anti-Cdk9 (phospho T186) antibody (ab79178)
Lane 1 : 3T3 cells treated with Forskolin (40 nM, 30 minutes)
Lane 2 : 3T3 cells treated with Forskolin (40 nM, 30 minutes) with phospho peptide
Predicted band size: 43 kDa
Observed band size: 43 kDa
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab79178 は 3 報の論文で使用されています。
- Huang Y et al. Human cytomegalovirus RNA2.7 inhibits RNA polymerase II (Pol II) Serine-2 phosphorylation by reducing the interaction between Pol II and phosphorylated cyclin-dependent kinase 9 (pCDK9). Virol Sin 37:358-369 (2022). PubMed: 35537980
- Li J et al. CDK9 inhibitor CDKI-73 is synergetic lethal with PARP inhibitor olaparib in BRCA1 wide-type ovarian cancer. Am J Cancer Res 10:1140-1155 (2020). PubMed: 32368391
- Pinto DO et al. Effect of transcription inhibition and generation of suppressive viral non-coding RNAs. Retrovirology 16:13 (2019). PubMed: 31036006