Anti-Cdk9 抗体 (ab6544)
Key features and details
- Rabbit polyclonal to Cdk9
- Suitable for: IHC-P, ELISA, IP, WB
- Reacts with: Mouse, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Cdk9 antibody
Cdk9 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Cdk9 -
由来種
Rabbit -
特異性
Antiserum will specifically react with a 43 kDa cdk9 (PITALRE) protein from human, rat and mouse tissue. No reaction was observed against other related cyclin dependent kinases. Cross reactivity with cdk9 (PITALRE) from other species may also occur. The murine cDNA is shown to be 98% identical with human. For immunohistochemistry use paraffin embedded tissue.
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アプリケーション
適用あり: IHC-P, ELISA, IP, WBmore details -
種交差性
交差種: Mouse, Human -
免疫原
Synthetic peptide corresponding to Human Cdk9. Multiple synthetic peptides corresponding to C-terminal and N-terminal domains.
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ポジティブ･コントロール
- WB: human kidney lysate, PC3 lysate IHC: mouse tissue
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特記事項
cdk9(PITALRE) interacts with a conserved domain in the TRAF-C region of the tumor necrosis factor signal transducer TRAF2. The positive transcription elongation factor b (P-TEFb) is identified as cdk9 paired with cyclin T1.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.01% Sodium azide
Constituents: 0.88% Sodium chloride, 0.42% Potassium phosphate -
Concentration information loading... -
精製度
Whole antiserum -
特記事項（精製）
This product was prepared from monospecific antiserum by delipidation and defibrination. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab6544の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P |
1/200 - 1/1000.
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| ELISA |
1/10000 - 1/50000.
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| IP |
1/100.
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| WB | (2) |
1/500 - 1/3000. Predicted molecular weight: 43 kDa.
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| 特記事項 |
|---|
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IHC-P
1/200 - 1/1000. |
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ELISA
1/10000 - 1/50000. |
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IP
1/100. |
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WB
1/500 - 1/3000. Predicted molecular weight: 43 kDa. |
ターゲット情報
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機能
Member of the cyclin-dependent kinase pair (CDK9/cyclin-T) complex, also called positive transcription elongation factor b (P-TEFb), which facilitates the transition from abortive to production elongation by phosphorylating the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAP II), SUPT5H and RDBP. The CDK9/cyclin-K complex has also a kinase activity toward CTD of RNAP II and can substitute for P-TEFb in vitro. -
組織特異性
Ubiquitous. -
配列類似性
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
Contains 1 protein kinase domain. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 1025 Human
- Entrez Gene: 107951 Mouse
- Omim: 603251 Human
- SwissProt: P50750 Human
- SwissProt: Q99J95 Mouse
- Unigene: 150423 Human
- Unigene: 706809 Human
- Unigene: 27557 Mouse
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別名
- C-2K antibody
- CDC2 related kinase antibody
- CDC2L4 antibody
see all
画像
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Western blot - Anti-Cdk9 antibody (ab6544)All lanes : Anti-Cdk9 antibody (ab6544) at 1/1000 dilution
Lane 1 : Opal PreStain 11-245 kDa (MB-210-0500) at 10 µl
Lane 2 : Human kidney lysate at 5 µg
Lane 3 : PC3 lysate at 20 µg
Lane 4 : Blank well
Predicted band size: 43 kDa
Exposure time: 46 seconds -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdk9 antibody (ab6544)Immunohistochemical staining of mouse tissue using anti-cdk9 (PITALRE) antiserum. The staining shows the location of cdk9 / PITALRE protein in developing mouse tissue. Arrow indicates area of high expression.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (9)
ab6544 は 9 報の論文で使用されています。
- Yang Y et al. Structural basis of RNA conformational switching in the transcriptional regulator 7SK RNP. Mol Cell 82:1724-1736.e7 (2022). PubMed: 35320752
- Sithole N et al. DDX5 potentiates HIV-1 transcription as a co-factor of Tat. Retrovirology 17:6 (2020). PubMed: 32228614
- Cary DC et al. HIV Transcription is Independent of Mediator Kinases. AIDS Res Hum Retroviruses N/A:N/A (2019). PubMed: 31044597
- Shan J et al. The C/ebp-Atf response element (CARE) location reveals two distinct Atf4-dependent, elongation-mediated mechanisms for transcriptional induction of aminoacyl-tRNA synthetase genes in response to amino acid limitation. Nucleic Acids Res 44:9719-9732 (2016). PubMed: 27471030
- Kuzmina A et al. A single point mutation in cyclin T1 eliminates binding to Hexim1, Cdk9 and RNA but not to AFF4 and enforces repression of HIV transcription. Retrovirology 11:51 (2014). WB . PubMed: 24985467
- Zhang H et al. SIRT2 directs the replication stress response through CDK9 deacetylation. Proc Natl Acad Sci U S A 110:13546-51 (2013). PubMed: 23898190
- Qi T et al. G-actin participates in RNA polymerase II-dependent transcription elongation by recruiting positive transcription elongation factor b (P-TEFb). J Biol Chem 286:15171-81 (2011). ICC/IF ; Human . PubMed: 21378166
- Kruse U et al. Chemoproteomics-based kinome profiling and target deconvolution of clinical multi-kinase inhibitors in primary chronic lymphocytic leukemia cells. Leukemia : (2010). PubMed: 20944678
- Iankova I et al. Peroxisome proliferator-activated receptor gamma recruits the positive transcription elongation factor b complex to activate transcription and promote adipogenesis. Mol Endocrinol 20:1494-505 (2006). PubMed: 16484339