Anti-CDK5RAP3 抗体 [EPR11293] (ab168353)
![Western blot - Anti-CDK5RAP3 antibody [EPR11293] (ab168353)](https://www.abcam.co.jp/ps/products/168/ab168353/Images/ab168353-166625-anti-cdk5rap3-antibody-epr11293-western-blot.jpg "Western blot - Anti-CDK5RAP3 antibody [EPR11293] (ab168353)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11293] to CDK5RAP3
- Suitable for: WB, ICC/IF
- Reacts with: Human
Related conjugates and formulations
製品の概要
-
製品名
Anti-CDK5RAP3 antibody [EPR11293]
CDK5RAP3 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR11293] to CDK5RAP3 -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IFmore details
適用なし: Flow Cyt or IHC-P -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat
-
免疫原
Synthetic peptide corresponding to Human CDK5RAP3.
-
ポジティブ･コントロール
- 293T, HeLa, U87-MG and A431 cell lysates; U87-MG cells; Immunoprecipitation pellet from 293T cell lysate.
-
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR11293 -
アイソタイプ
IgG -
研究分野
関連製品
-
Alternative Versions
-
Isotype control
-
Positive Controls
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab168353の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000 - 1/5000. Predicted molecular weight: 57 kDa.
|
|
| ICC/IF |
1/50 - 1/100.
|
| 特記事項 |
|---|
|
WB
1/1000 - 1/5000. Predicted molecular weight: 57 kDa. |
|
ICC/IF
1/50 - 1/100. |
追加情報
Is unsuitable for Flow Cyt or IHC-P.
ターゲット情報
-
機能
Potential regulator of CDK5 activity. May be involved in cell proliferation. Regulates CDK5 activity via its interaction with CDK5R1. -
組織特異性
Ubiquitous. Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Isoform 3 is expressed in kidney, liver, skeletal muscle and placenta. -
配列類似性
Belongs to the CDK5RAP3 family. -
翻訳後修飾
Phosphorylated in vitro by CDK5. - Information by UniProt
-
参照データベース
- Entrez Gene: 80279 Human
- Entrez Gene: 80280 Mouse
- Entrez Gene: 80278 Rat
- Omim: 608202 Human
- SwissProt: Q96JB5 Human
- SwissProt: Q99LM2 Mouse
- SwissProt: Q9JLH7 Rat
- Unigene: 20157 Human
see all -
別名
- C53 antibody
- CDK5 activator binding protein C53 antibody
- CDK5 activator-binding protein C53 antibody
see all
画像
-
Western blot - Anti-CDK5RAP3 antibody [EPR11293] (ab168353)All lanes : Anti-CDK5RAP3 antibody [EPR11293] (ab168353) at 1/1000 dilution
Lane 1 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 3 : U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) whole cell lysate
Lane 4 : A-431 (Human epidermoid carcinoma cell line) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labeled goat anti-rabbit at 1/2000 dilution
Predicted band size: 57 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-CDK5RAP3 antibody [EPR11293] (ab168353)](https://www.abcam.co.jp/ps/products/168/ab168353/Images/ab168353-166624-anti-cdk5rap3-antibody-epr11293-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CDK5RAP3 antibody [EPR11293] (ab168353)Immunofluorescent analysis of U87-MG cells labeling CDK5RAP3 with ab168353 at 1/50 dilution. -
![Western blot - Anti-CDK5RAP3 antibody [EPR11293] (ab168353)](https://www.abcam.co.jp/ps/products/168/ab168353/Images/ab168353-166623-anti-cdk5rap3-antibody-epr11293-western-blot.jpg)
Western blot - Anti-CDK5RAP3 antibody [EPR11293] (ab168353)Anti-CDK5RAP3 antibody [EPR11293] (ab168353) at 1/1000 dilution + immunoprecipitation pellet from 293T cell lysate
Secondary
HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG
Predicted band size: 57 kDa -
![Anti-CDK5RAP3 antibody [EPR11293] (ab168353)](https://www.abcam.co.jp/ps/products/168/ab168353/Images/ab168353-4-benefits-of-recombinant-antibodies.png)
Anti-CDK5RAP3 antibody [EPR11293] (ab168353)
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (1)
ab168353 は 1 報の論文で使用されています。
- Chen QY et al. CDK5RAP3 Inhibits the Translocation of MCM6 to Influence the Prognosis in Gastric Cancer. J Cancer 10:4488-4498 (2019). PubMed: 31528213