Anti-CDK5RAP2 抗体 (ab86340)
Key features and details
- Rabbit polyclonal to CDK5RAP2
- Suitable for: ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-CDK5RAP2 antibody
CDK5RAP2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to CDK5RAP2 -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IFmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to a region between residues 1843 and the C-terminus (residue 1893) of Human CDK5RAP2 (NP_060719.4)
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ポジティブ･コントロール
- HeLa cells
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab86340の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
| 特記事項 |
|---|
追加情報
ICC/IF: 1/100 - 1/500. Fix with formaldehyde of methanol. Do not fix with acetone. Permeabilization with Triton-X 100 recommended.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
ターゲット情報
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機能
Potential regulator of CDK5 activity via its interaction with CDK5R1. Negative regulator of centriole disengagement (licensing) which maintains centriole engagement and cohesion. Involved in regulation of mitotic spindle orientation (By similarity). Plays a role in the spindle checkpoint activation by acting as a transcriptional regulator of both BUBR1 and MAD2 promoter. Together with MAPRE1, it may promote microtubule polymerization, bundle formation, growth and dynamics at the plus ends. -
組織特異性
Widely expressed. Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. -
関連疾患
Defects in CDK5RAP2 are the cause of microcephaly primary type 3 (MCPH3) [MIM:604804]. A disorder defined as a head circumference more than 3 standard deviations below the age-related mean. Brain weight is markedly reduced and the cerebral cortex is disproportionately small. Despite this marked reduction in size, the gyral pattern is relatively well preserved, with no major abnormality in cortical architecture. Primary microcephaly is further defined by the absence of other syndromic features or significant neurological deficits. -
翻訳後修飾
Phosphorylated in vitro by CDK5. -
細胞内局在
Cytoplasm > cytoskeleton > centrosome. Golgi apparatus. Cytoplasm. Found in the pericentriolar region adhering to the surface of the centrosome and in the region of the centrosomal appendages. Localizes to microtubule plus ends. - Information by UniProt
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参照データベース
- Entrez Gene: 55755 Human
- Omim: 608201 Human
- SwissProt: Q96SN8 Human
- Unigene: 269560 Human
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別名
- C48 antibody
- CDK5 activator-binding protein C48 antibody
- CDK5 regulatory subunit associated protein 2 antibody
see all
画像
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Immunocytochemistry/ Immunofluorescence - Anti-CDK5RAP2 antibody (ab86340)ab86340 at 1/250 dilution staining CDK5RAP2 in NBF-fixed asynchronous HeLa cells by Imunofluorescence.
Detection: Anti-rabbit IgG-FITC conjugated used at a dilution of 1/100. -
Immunocytochemistry/ Immunofluorescence - Anti-CDK5RAP2 antibody (ab86340)ab86340 at 1/250 dilution staining CDK5RAP2 in NBF-fixed asynchronous HeLa cells by Imunofluorescence.
Detection: Anti-rabbit IgG-FITC conjugated used at a dilution of 1/100.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (7)
ab86340 は 7 報の論文で使用されています。
- Lee HJ et al. Idebenone Regulates Aβ and LPS-Induced Neurogliosis and Cognitive Function Through Inhibition of NLRP3 Inflammasome/IL-1β Axis Activation. Front Immunol 13:749336 (2022). PubMed: 35222363
- Kim J et al. Sorafenib Modulates the LPS- and Aß-Induced Neuroinflammatory Response in Cells, Wild-Type Mice, and 5xFAD Mice. Front Immunol 12:684344 (2021). PubMed: 34122447
- Meitinger F et al. TRIM37 prevents formation of condensate-organized ectopic spindle poles to ensure mitotic fidelity. J Cell Biol 220:N/A (2021). PubMed: 33983387
- Meitinger F et al. TRIM37 controls cancer-specific vulnerability to PLK4 inhibition. Nature 585:440-446 (2020). PubMed: 32908304
- Watanabe S et al. Centriole-independent mitotic spindle assembly relies on the PCNT-CDK5RAP2 pericentriolar matrix. J Cell Biol 219:N/A (2020). PubMed: 33170211
- Munkley J et al. The androgen receptor controls expression of the cancer-associated sTn antigen and cell adhesion through induction of ST6GalNAc1 in prostate cancer. Oncotarget 6:34358-74 (2015). PubMed: 26452038
- Astuti D et al. Germline mutations in DIS3L2 cause the Perlman syndrome of overgrowth and Wilms tumor susceptibility. Nat Genet 44:277-84 (2012). PubMed: 22306653