Anti-CDK5RAP2 抗体 (ab70213)
Key features and details
- Rabbit polyclonal to CDK5RAP2
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-CDK5RAP2 antibody
CDK5RAP2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to CDK5RAP2 -
由来種
Rabbit -
特異性
We have data to indicate that this antibody may not cross react with Mouse. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues. -
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Human
交差が予測される動物種: Chimpanzee, Gorilla, African green monkey
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免疫原
Synthetic peptide correponding to a region between residues 900 and 950 of human CDK5RAP2
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ポジティブ･コントロール
- Whole cell lysate from HeLa cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab70213の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/2500 - 1/15000. Detects a band of approximately 238 kDa (predicted molecular weight: 215 kDa).
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| IP |
Use at 3-5 µg/mg of lysate.
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| 特記事項 |
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WB
1/2500 - 1/15000. Detects a band of approximately 238 kDa (predicted molecular weight: 215 kDa). |
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IP
Use at 3-5 µg/mg of lysate. |
ターゲット情報
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機能
Potential regulator of CDK5 activity via its interaction with CDK5R1. Negative regulator of centriole disengagement (licensing) which maintains centriole engagement and cohesion. Involved in regulation of mitotic spindle orientation (By similarity). Plays a role in the spindle checkpoint activation by acting as a transcriptional regulator of both BUBR1 and MAD2 promoter. Together with MAPRE1, it may promote microtubule polymerization, bundle formation, growth and dynamics at the plus ends. -
組織特異性
Widely expressed. Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. -
関連疾患
Defects in CDK5RAP2 are the cause of microcephaly primary type 3 (MCPH3) [MIM:604804]. A disorder defined as a head circumference more than 3 standard deviations below the age-related mean. Brain weight is markedly reduced and the cerebral cortex is disproportionately small. Despite this marked reduction in size, the gyral pattern is relatively well preserved, with no major abnormality in cortical architecture. Primary microcephaly is further defined by the absence of other syndromic features or significant neurological deficits. -
翻訳後修飾
Phosphorylated in vitro by CDK5. -
細胞内局在
Cytoplasm > cytoskeleton > centrosome. Golgi apparatus. Cytoplasm. Found in the pericentriolar region adhering to the surface of the centrosome and in the region of the centrosomal appendages. Localizes to microtubule plus ends. - Information by UniProt
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参照データベース
- Entrez Gene: 464695 Chimpanzee
- Entrez Gene: 55755 Human
- Omim: 608201 Human
- SwissProt: Q19UN5 Chimpanzee
- SwissProt: Q96SN8 Human
- Unigene: 269560 Human
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別名
- C48 antibody
- CDK5 activator-binding protein C48 antibody
- CDK5 regulatory subunit associated protein 2 antibody
see all
画像
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Western blot - Anti-CDK5RAP2 antibody (ab70213)All lanes : Anti-CDK5RAP2 antibody (ab70213) at 0.1 µg/ml
Lane 1 : Whole cell lysate from HeLa cells at 50 µg
Lane 2 : Whole cell lysate from HeLa cells at 15 µg
Lane 3 : Whole cell lysate from HeLa cells at 5 µg
Lane 4 : Whole cell lysate from NIH3T3 cells at 50 µg
Developed using the ECL technique.
Predicted band size: 215 kDa
Observed band size: 238 kDa why is the actual band size different from the predicted?
Additional bands at: 330 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds -
Immunoprecipitation - Anti-CDK5RAP2 antibody (ab70213)Detection of Human CDK5RAP2 by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg/IP, 1/4 of IP loaded) using affinity purified rabbit anti-CDK5RAP2 antibody ab70213 at 3 µg/mg of lysate for IP and at 0.1 µg/ml for subsequent WB detection. Note: bands between 55 and 71 kDa represent the IgG chains.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab70213 は 4 報の論文で使用されています。
- Huang F et al. Cartwheel disassembly regulated by CDK1-cyclin B kinase allows human centriole disengagement and licensing. J Biol Chem 298:102658 (2022). PubMed: 36356903
- Chi W et al. PLK4-phosphorylated NEDD1 facilitates cartwheel assembly and centriole biogenesis initiations. J Cell Biol 220:N/A (2021). PubMed: 33351100
- Tungadi EA et al. Human microcephaly ASPM protein is a spindle pole-focusing factor that functions redundantly with CDK5RAP2. J Cell Sci 130:3676-3684 (2017). PubMed: 28883092
- Cervenka I et al. Dishevelled is a NEK2 kinase substrate controlling dynamics of centrosomal linker proteins. Proc Natl Acad Sci U S A 113:9304-9 (2016). WB, ICC/IF . PubMed: 27486244