AB70213

Anti-CDK5RAP2 抗体

Name: Anti-CDK5RAP2 抗体 (ab70213) | Abcam
Brand: Abcam Limited
SKU: AB70213
Price: 76000 JPY
Availability: InStock

Anti-CDK5RAP2 antibody

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(6 Publications)

Rabbit Polyclonal CDK5RAP2 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human CDK5RAP2.

別名を表示する

CEP215, KIAA1633, CDK5RAP2, CDK5 regulatory subunit-associated protein 2, CDK5 activator-binding protein C48, Centrosome-associated protein 215

2 Images

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Immunoprecipitation - Anti-CDK5RAP2 antibody (AB70213)

Detection of Human CDK5RAP2 by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg/IP, 1/4 of IP loaded) using affinity purified rabbit anti-CDK5RAP2 antibody ab70213 at 3 µg/mg of lysate for IP and at 0.1 µg/ml for subsequent WB detection. Note : bands between 55 and 71 kDa represent the IgG chains.

All lanes:

Immunoprecipitation - Anti-CDK5RAP2 antibody (ab70213)

Predicted band size: 215 kDa

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Western blot - Anti-CDK5RAP2 antibody (AB70213)

All lanes:

Western blot - Anti-CDK5RAP2 antibody (ab70213) at 0.1 µg/mL

Lane 1:

Whole cell lysate from HeLa cells at 50 µg

Lane 2:

Whole cell lysate from HeLa cells at 15 µg

Lane 3:

Whole cell lysate from HeLa cells at 5 µg

Lane 4:

Whole cell lysate from NIH3T3 cells at 50 µg

Predicted band size: 215 kDa

Observed band size: 238 kDa,330 kDa

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Exposure time: 30s

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

WB, IP

applications

免疫原

Synthetic Peptide within Human CDK5RAP2. The exact immunogen used to generate this antibody is proprietary information.

Q96SN8

特異性

We have data to indicate that this antibody may not cross react with Mouse. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "3-5 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2500 - 1/15000", "WB-species-notes": "<p></p>" }, "African green monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Immunogen

バッファー組成

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: PBS, 1.815% Tris, 1.764% Sodium citrate

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Potential regulator of CDK5 activity via its interaction with CDK5R1 (PubMed : 15164053). Negative regulator of centriole disengagement (licensing) which maintains centriole engagement and cohesion. Involved in regulation of mitotic spindle orientation (By similarity). Plays a role in the spindle checkpoint activation by acting as a transcriptional regulator of both BUBR1 and MAD2 promoter (PubMed : 19282672). Together with EB1/MAPRE1, may promote microtubule polymerization, bundle formation, growth and dynamics at the plus ends (PubMed : 18042621, PubMed : 17959831, PubMed : 19553473). Regulates centrosomal maturation by recruitment of the gamma-tubulin ring complex (gTuRC) onto centrosomes (PubMed : 18042621, PubMed : 17959831, PubMed : 26485573, PubMed : 39321809). In complex with PDE4DIP isoform 13/MMG8/SMYLE, MAPRE1 and AKAP9, contributes to microtubules nucleation and extension from the centrosome to the cell periphery (PubMed : 29162697). Required for the recruitment of AKAP9 to centrosomes (PubMed : 29162697). Plays a role in neurogenesis (By similarity).

See full target information CDK5RAP2

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文献 (6)

Recent publications for all applications. Explore the full list and refine your search

Science advances 10:eadn0086 PubMed39661677

2024

MLL/WDR5 complex recruits centriolar satellite protein Cep72 to regulate microtubule nucleation and spindle formation.

Applications

Unspecified application

Species

Unspecified reactive species

Swathi Chodisetty,Aditi Arora,Kausika Kumar Malik,Himanshu Goel,Shweta Tyagi

Cell death & disease 14:107 PubMed36774351

2023

CDK5RAP2 is a Wnt target gene and promotes stemness and progression of oral squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Yuehong Shen,Yuling Chen,Yuntao Lin,Yicun Li,Pengfei Liu,Biru Zhang,Yufan Wang,King-Chi Chan,Nai-Ki Mak,Michael Kahn,Robert Z Qi,Hongyu Yang

The Journal of biological chemistry 298:102658 PubMed36356903

2022

Cartwheel disassembly regulated by CDK1-cyclin B kinase allows human centriole disengagement and licensing.

Applications

Unspecified application

Species

Unspecified reactive species

Fan Huang,Xiaowei Xu,Guangwei Xin,Boyan Zhang,Qing Jiang,Chuanmao Zhang

The Journal of cell biology 220: PubMed33351100

2020

PLK4-phosphorylated NEDD1 facilitates cartwheel assembly and centriole biogenesis initiations.

Applications

Unspecified application

Species

Unspecified reactive species

Wangfei Chi,Gang Wang,Guangwei Xin,Qing Jiang,Chuanmao Zhang

Journal of cell science 130:3676-3684 PubMed28883092

2017

Human microcephaly ASPM protein is a spindle pole-focusing factor that functions redundantly with CDK5RAP2.

Applications

Unspecified application

Species

Unspecified reactive species

Elsa A Tungadi,Ami Ito,Tomomi Kiyomitsu,Gohta Goshima

Proceedings of the National Academy of Sciences of 113:9304-9 PubMed27486244

2016

Dishevelled is a NEK2 kinase substrate controlling dynamics of centrosomal linker proteins.

Applications

WB, ICC/IF

Species

Unspecified reactive species, Unspecified reactive species

Igor Cervenka,Jana Valnohova,Ondrej Bernatik,Jakub Harnos,Matej Radsetoulal,Katerina Sedova,Katerina Hanakova,David Potesil,Miroslava Sedlackova,Alena Salasova,Zachary Steinhart,Stephane Angers,Gunnar Schulte,Ales Hampl,Zbynek Zdrahal,Vitezslav Bryja

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CDK5RAP2 抗体

Anti-CDK5RAP2 antibody

Immunoprecipitation - Anti-CDK5RAP2 antibody (AB70213)

Western blot - Anti-CDK5RAP2 antibody (AB70213)

Key facts

宿主種

クローン性

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

特異性

Reactivity data

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

製品プロトコール

ターゲットの情報

文献 (6)

代替製品

Primary Antibodies

組合せ製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise