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リコンビナントRabMAb

Anti-Cdk4 抗体 [EPR2513Y] (ab68266)

Submit a review Submit a question References (21)
Western blot - Anti-Cdk4 antibody [EPR2513Y] (ab68266)
  • Immunocytochemistry/ Immunofluorescence - Anti-Cdk4 antibody [EPR2513Y] (ab68266)
  • Immunoprecipitation - Anti-Cdk4 antibody [EPR2513Y] (ab68266)
  • Flow Cytometry (Intracellular) - Anti-Cdk4 antibody [EPR2513Y] (ab68266)
  • Anti-Cdk4 antibody [EPR2513Y] (ab68266)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2513Y] to Cdk4
  • Suitable for: Flow Cyt (Intra), ICC/IF, WB, IP
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Carrier Free PE

製品の概要

  • 製品名

    Anti-Cdk4 antibody [EPR2513Y]
    Cdk4 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR2513Y] to Cdk4

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: Flow Cyt (Intra), ICC/IF, WB, IPmore details
    適用なし: IHC-P

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide corresponding to Human Cdk4 aa 1-100 (N terminal).

  • ポジティブ･コントロール

    • WB: HeLa, Ramos, and K562 cell lysates. Rat and mouse lung tissue lysates. ICC/IF: MCF7 cells. Flow Cyt (intra) (Intra): A549 cells. IP: K562 whole cell lysate.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab68266の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt (Intra)
1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF
1/100.
WB
1/500 - 1/1000. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa).
IP
1/30.
特記事項
Flow Cyt (Intra)
1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF
1/100.
WB
1/500 - 1/1000. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa).
IP
1/30.
追加情報
Is unsuitable for IHC-P.

ターゲット情報

  • 機能

    Ser/Thr-kinase component of cyclin D-CDK4 (DC) complexes that phosphorylate and inhibit members of the retinoblastoma (RB) protein family including RB1 and regulate the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complexes and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also phosphorylates SMAD3 in a cell-cycle-dependent manner and represses its transcriptional activity. Component of the ternary complex, cyclin D/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex.

  • 関連疾患

    Defects in CDK4 are a cause of susceptibility to cutaneous malignant melanoma type 3 (CMM3) [MIM:609048]. Malignant melanoma is a malignant neoplasm of melanocytes, arising de novo or from a pre-existing benign nevus, which occurs most often in the skin but also may involve other sites.

  • 配列類似性

    Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
    Contains 1 protein kinase domain.

  • 翻訳後修飾

    Phosphorylation at Thr-172 is required for enzymatic activity. Phosphorylated, in vitro, at this site by CCNH-CDK7, but, in vivo, appears to be phosphorylated by a proline-directed kinase. In the cyclin D-CDK4-CDKN1B complex, this phosphorylation and consequent CDK4 enzyme activity, is dependent on the tyrosine phosphorylation state of CDKN1B. Thus, in proliferating cells, CDK4 within the complex is phosphorylated on Thr-172 in the T-loop. In resting cells, phosphorylation on Thr-172 is prevented by the non-tyrosine-phosphorylated form of CDKN1B.

  • 細胞内局在

    Cytoplasm. Nucleus. Membrane. Cytoplasmic when non-complexed. Forms a cyclin D-CDK4 complex in the cytoplasm as cells progress through G(1) phase. The complex accumulates on the nuclear membrane and enters the nucleus on transition from G(1) to S phase. Also present in nucleoli and heterochromatin lumps. Colocalizes with RB1 after release into the nucleus.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • Cdk 4 antibody
    • cdk4 antibody
    • CDK4 protein antibody
    • CDK4_HUMAN antibody
    • Cell division kinase 4 antibody
    • Cell division protein kinase 4 antibody
    • CMM 3 antibody
    • CMM3 antibody
    • Crk3 antibody
    • Cyclin dependent kinase 4 antibody
    • Cyclin-dependent kinase 4 antibody
    • Melanoma cutaneous malignant 3 antibody
    • MGC14458 antibody
    • p34 cdk4 antibody
    • PSK J3 antibody
    • PSK-J3 antibody
    see all

画像

  • Western blot - Anti-Cdk4 antibody [EPR2513Y] (ab68266)
    Western blot - Anti-Cdk4 antibody [EPR2513Y] (ab68266)
    All lanes : Anti-Cdk4 antibody [EPR2513Y] (ab68266) at 1/2000 dilution

    Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
    Lane 2 : K562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate
    Lane 3 : Ramos (Human Burkitt's lymphoma B lymphocyte) whole cell lysate
    Lane 4 : Rat lung lysate
    Lane 5 : Mouse lung lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 34 kDa
    Additional bands at: 34 kDa. We are unsure as to the identity of these extra bands.



    Exposure times:  Lane 1-3: 30 secs; Lane 4-5: 180 secs.

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunocytochemistry/ Immunofluorescence - Anti-Cdk4 antibody [EPR2513Y] (ab68266)
    Immunocytochemistry/ Immunofluorescence - Anti-Cdk4 antibody [EPR2513Y] (ab68266)

    Immunocytochemistry/ Immunofluorescence analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labeling Cdk4 with ab68266 at 1:100 (5.4 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% triton X-100. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain.

    PBS instead of the primary antibody was used as the secondary antibody only control.

  • Immunoprecipitation - Anti-Cdk4 antibody [EPR2513Y] (ab68266)
    Immunoprecipitation - Anti-Cdk4 antibody [EPR2513Y] (ab68266)

    ab68266 at 1:30 dilution (2 µg) immunoprecipitating Cdk4 in K562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate.
    Lane 1 (input): K562 whole cell lysate 10 µg
    Lane 2 (+): ab68266 & K562 whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab68266 in K562 whole cell lysate
    For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST.

  • Flow Cytometry (Intracellular) - Anti-Cdk4 antibody [EPR2513Y] (ab68266)
    Flow Cytometry (Intracellular) - Anti-Cdk4 antibody [EPR2513Y] (ab68266)

    Intracellular Flow Cytometry analysis of A549 (Human lung carcinoma epithelial cell) cells labeling Cdk4 with ab68266 at 1/50 dilution (1µg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue). 

     

     

  • Anti-Cdk4 antibody [EPR2513Y] (ab68266)
    Anti-Cdk4 antibody [EPR2513Y] (ab68266)

参考文献 (21)

ab68266 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab68266 は 21 報の論文で使用されています。

  • Zhu Q  et al. 7-Ketocholesterol accelerates pancreatic β-cell senescence by inhibiting the SIRT1/CDK4-Rb-E2F1 signaling pathway. Islets 15:2219105 (2023). PubMed: 37265106
  • Leung CON  et al. Broad-spectrum kinome profiling identifies CDK6 upregulation as a driver of lenvatinib resistance in hepatocellular carcinoma. Nat Commun 14:6699 (2023). PubMed: 37872167
  • Fu Q  et al. Long non-coding RNA-PCGEM1 contributes to prostate cancer progression by sponging microRNA miR-129-5p to enhance chromatin licensing and DNA replication factor 1 expression. Bioengineered 13:9411-9424 (2022). PubMed: 35412947
  • Gharbi SI  et al. Crystal structure of active CDK4-cyclin D and mechanistic basis for abemaciclib efficacy. NPJ Breast Cancer 8:126 (2022). PubMed: 36446794
  • Lv T  et al. Knockdown of lncRNA DLEU1 inhibits the tumorigenesis of oral squamous cell carcinoma via regulation of miR-149-5p/CDK6 axis. Mol Med Rep 23:N/A (2021). PubMed: 33880596
View all Publications for this product

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