AB137675

Anti-Cdk4 抗体

Name: Anti-Cdk4 抗体 (ab137675) | Abcam
Brand: Abcam Limited
SKU: AB137675
Price: 76000 JPY
Availability: InStock
Rating: 5 (1 reviews)

Anti-Cdk4 antibody

(1 Review)

(96 Publications)

Rabbit Polyclonal CDK4 antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 96 publications. Immunogen corresponding to Recombinant Fragment Protein within Human CDK4 aa 100 to C-terminus.

別名を表示する

Cyclin-dependent kinase 4, Cell division protein kinase 4, PSK-J3, CDK4

6 Images

Immunocytochemistry/ Immunofluorescence - Anti-Cdk4 antibody (AB137675)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Cdk4 antibody (AB137675)

HeLa cells stained for CDK4 (green) using ab137675 at 1/500 dilution in ICC/IF.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Cdk4 antibody (AB137675)

MCF7 cells stained for CDK4 (green) using ab137675 at 1/1000 dilution in ICC/IF. Red staining of p21 Cip1 (a nucleus marker) at 1/500 dilution and Hoechst 33342 (blue) staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdk4 antibody (AB137675)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdk4 antibody (AB137675)

Immunohistochemical analysis of paraffin-embedded Human gastric carcinoma tissue, labelling Cdk4 using ab137675 at 1/500 dilution.

AbReview58781****

Western blot - Anti-Cdk4 antibody (AB137675)

10% SDS-PAGE.

Blocked with 5% milk for 12 hours at 4°C.

Incubated with the primary antibody in 2% milk for 12 hours at 4°C.

All lanes:

Western blot - Anti-Cdk4 antibody (ab137675) at 1/1000 dilution

Lane 1:

Epithelial OVCAR whole cell lysate - untreated at 100 µg

Lane 2:

Epithelial OVCAR whole cell lysate treated with 100µM Perifosine for 24 hours at 100 µg

Lane 3:

Epithelial OVCAR whole cell lysate treated with 200µM Perifosine for 24 hours at 100 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG at 1/10000 dilution

Predicted band size: 34 kDa

Observed band size: 30 kDa

true

Exposure time: 5min

This image is courtesy of an anonymous Abreview.

Supplier Data

Western blot - Anti-Cdk4 antibody (AB137675)

12% SDS-PAGE

All lanes:

Western blot - Anti-Cdk4 antibody (ab137675) at 1/500 dilution

All lanes:

PC-12 whole cell extract at 30 µg

Predicted band size: 34 kDa

false

Supplier Data

Western blot - Anti-Cdk4 antibody (AB137675)

Samples were separated by 12% SDS-PAGE.

All lanes:

Western blot - Anti-Cdk4 antibody (ab137675) at 1/500 dilution

All lanes:

NIH-3T3 Whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 34 kDa

false

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Mouse, Rat, Human

アプリケーション

WB, ICC/IF, IHC-P

applications

免疫原

Recombinant Fragment Protein within Human CDK4 aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P11802

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Immunogen

バッファー組成

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Cdk4 also known as Cyclin-dependent kinase 4 is a serine/threonine kinase involved in cell cycle regulation. Cdk4 has a molecular weight of approximately 34 kDa. This protein mainly locates in the nucleus of the cell and its expression occurs in diverse tissues. Cdk4 partners with D-type cyclins to exert its function which is essential for the transition from the G1 phase to the S phase of the cell cycle. Researchers often study Cdk4 using techniques like immunohistochemistry (IHC) to determine its expression and localization in different tissues.

Biological function summary

Cdk4 plays a fundamental role in cell cycle regulation by forming a complex with D-type cyclins. Together they phosphorylate the retinoblastoma protein (Rb) resulting in the release of E2F transcription factors which promote the expression of genes necessary for DNA replication. The Cdk4/cyclin D complex regulates the cell's commitment to enter S phase and continue cell division. This regulation is key for normal cell proliferation and tissue homeostasis. Cdk4 activity is strictly controlled by INK4 family members acting as inhibitors and adding an extra regulation layer.

Pathways

Cdk4 is an integral part of important signaling pathways like the cell cycle and PI3K/AKT pathways. In the cell cycle pathway Cdk4 relays signals downstream that drive the transition from the G1 to S phase through its interaction with cyclin D and Rb. In the PI3K/AKT pathway signaling can influence cyclin D expression indirectly affecting Cdk4 activity. Proteins such as Cdk6 closely relate to Cdk4 and often compensate or partner with Cdk4 in these pathways to maintain proper cell cycle progression.

Abnormalities in Cdk4 function or regulation relate to cancer and certain metabolic syndromes. Hyperactivity of Cdk4 due to overexpression or mutation frequently occurs in several cancers including melanoma and breast cancer. In these malignancies Cdk4 aberrantly drives excessive cell proliferation. Mutations or alterations in proteins such as p16 an inhibitor of Cdk4 are often found in these cancers highlighting the critical role of Cdk4 in disease progression. Understanding the role of Cdk4 allows researchers to develop targeted therapies such as Cdk4/6 inhibitors offering new treatment options.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Ser/Thr-kinase component of cyclin D-CDK4 (DC) complexes that phosphorylate and inhibit members of the retinoblastoma (RB) protein family including RB1 and regulate the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complexes and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also phosphorylates SMAD3 in a cell-cycle-dependent manner and represses its transcriptional activity. Component of the ternary complex, cyclin D/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex.

See full target information CDK4

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文献 (96)

Recent publications for all applications. Explore the full list and refine your search

Experimental & molecular medicine 57:1996-2009 PubMed40887499

2025

Novel small molecules downregulate CDK1 expression and inhibit Wnt/β-catenin signaling in cutaneous squamous cell carcinoma by targeting its distinct tumor-specific cellular landscape.

Applications

Unspecified application

Species

Unspecified reactive species

Soung-Hoon Lee,Min-Jeong Kang,Mi Ryung Roh,Kang-Yell Choi

Carcinogenesis 46: PubMed40823720

2025

Core transcriptional regulatory circuit-regulated IGF2BP3 stabilizes E2F2 mRNA via m6A modification in neuroblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Pengcheng Yang,Jianwei Wang,Ziheng Wu,Ran Zhuo,Yanling Chen,Gen Li,Yanfang Tao,Xiaolu Li,Fang Fang,Di Wu,Yang Yang,Hongli Yin,Guanghui Qian,Hairong Wang,Xin Li,Juanjuan Yu,Randong Yang,Yunyun Xu,Zhiheng Li,Lei Shi,Zimu Zhang,Jian Pan,Jian Wang

Journal of experimental & clinical cancer research : CR 44:170 PubMed40462177

2025

PSMB10 maintains the stemness of chemotherapeutic drug-resistant leukemia cells by inhibiting senescence and cytotoxic T lymphocyte-mediated killing in a ubiquitinated degradation manner.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao Ma,Yingnan Li,Di Wang,Jialan Niu,Qian Li,Ying Chen,Mengyuan Wang,Jin Wen,Chenxi Liao,Nan Wang,Xiaolan Zhang,Jiwei Chang,Jiayi Yang,Lei Li,Jing Zou,Danyue Peng,Lingbo Liu

Cancers 17: PubMed39796711

2025

DNAJB1-PKAc Kinase Is Expressed in Young Patients with Pediatric Liver Cancers and Enhances Carcinogenic Pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Yasmeen Fleifil,Ruhi Gulati,Katherine Jennings,Alexander Miethke,Alexander Bondoc,Gregory Tiao,James I Geller,Rebekah Karns,Lubov Timchenko,Nikolai Timchenko

Cell & bioscience 14:109 PubMed39210450

2024

MST1R-targeted therapy in the battle against gallbladder cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Wang,Chao Huang,Li Zhang,Liqin Yu,Yangming Liu,Puxiongzhi Wang,Rongmu Xia

Cell 187:1666-1684.e26 PubMed38490194

2024

First-in-class MKK4 inhibitors enhance liver regeneration and prevent liver failure.

Applications

Unspecified application

Species

Unspecified reactive species

Stefan Zwirner,Anan A Abu Rmilah,Sabrina Klotz,Bent Pfaffenroth,Philip Kloevekorn,Athina A Moschopoulou,Svenja Schuette,Mathias Haag,Roland Selig,Kewei Li,Wei Zhou,Erek Nelson,Antti Poso,Harvey Chen,Bruce Amiot,Yao Jia,Anna Minshew,Gregory Michalak,Wei Cui,Elke Rist,Thomas Longerich,Birgit Jung,Philipp Felgendreff,Omelyan Trompak,Prem K Premsrirut,Katharina Gries,Thomas E Muerdter,Georg Heinkele,Torsten Wuestefeld,David Shapiro,Markus Weissbach,Alfred Koenigsrainer,Bence Sipos,Eiso Ab,Magdalena Ortiz Zacarias,Stephan Theisgen,Nicole Gruenheit,Saskia Biskup,Matthias Schwab,Wolfgang Albrecht,Stefan Laufer,Scott Nyberg,Lars Zender

American journal of cancer research 13:2323-2341 PubMed37424824

2023

USP37 promotes angiogenesis and metastasis in colorectal cancer by facilitating β-catenin stability.

Applications

Unspecified application

Species

Unspecified reactive species

Dafeng Tong,Jie Yuan,Zhaoming Wang,Enda Yu,Jifu E

Cell death & disease 14:314 PubMed37160873

2023

TFEB inhibition induces melanoma shut-down by blocking the cell cycle and rewiring metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

C Ariano,F Costanza,M Akman,C Riganti,D Corà,E Casanova,E Astanina,V Comunanza,F Bussolino,G Doronzo

British journal of pharmacology 180:2172-2188 PubMed36967633

2023

Palbociclib blocks neutrophilic phosphatidylinositol 3-kinase activity to alleviate psoriasiform dermatitis.

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Unspecified application

Species

Unspecified reactive species

Po-Jen Chen,Hsin-Hui Tseng,Yi-Hsuan Wang,Shu-Yen Fang,Shun-Hua Chen,Chun-Hong Chen,Sheng-Chieh Tsai,Yu-Chia Chang,Yung-Fong Tsai,Tsong-Long Hwang

Cell reports 42:112235 PubMed36920905

2023

Single-cell heterogeneity of EGFR and CDK4 co-amplification is linked to immune infiltration in glioblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Kacper A Walentynowicz,Dalit Engelhardt,Simona Cristea,Shreya Yadav,Ugoma Onubogu,Roberto Salatino,Melanie Maerken,Cristina Vincentelli,Aashna Jhaveri,Jacob Geisberg,Thomas O McDonald,Franziska Michor,Michalina Janiszewska

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