Anti-CDK1 (phospho Y15) 抗体 (ab47594)
Key features and details
- Rabbit polyclonal to CDK1 (phospho Y15)
- Suitable for: WB
- Reacts with: Human, African green monkey
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-CDK1 (phospho Y15) antibody
CDK1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to CDK1 (phospho Y15) -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human, African green monkey -
免疫原
Synthetic peptide corresponding to Human CDK1 aa 1-100 (phospho Y15).
Database link: P06493 -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab47594の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (1) |
1/500 - 1/1000. Detects a band of approximately 35 kDa (predicted molecular weight: 34 kDa).
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| 特記事項 |
|---|
|
WB
1/500 - 1/1000. Detects a band of approximately 35 kDa (predicted molecular weight: 34 kDa). |
ターゲット情報
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機能
Plays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. p34 is a component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II. -
組織特異性
Isoform 2 is found in breast cancer tissues. -
配列類似性
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
Contains 1 protein kinase domain. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 983 Human
- Omim: 116940 Human
- SwissProt: P06493 Human
- Unigene: 334562 Human
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製品の状態
CDK1 can be located to the Nucleus, cytoplasm and Mithocondria. It's cytoplasmic during interphase and reversibly translocated from cytoplasm to the nucleus when phosphorilated before G2-M transition when associated with cyclin-B1. Accumulates in mitochondria in G2-arrested cells upon DNA-damage. -
別名
- Cdc 2 antibody
- Cdc2 antibody
- CDC28A antibody
see all
画像
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Western blot - Anti-CDK1 (phospho Y15) antibody (ab47594)Anti-CDK1 (phospho Y15) antibody (ab47594) at 1/500 dilution + COS-7 cells (African green monkey kidney fibroblast-like)
Predicted band size: 34 kDa -
Western blot - Anti-CDK1 (phospho Y15) antibody (ab47594)Anti-CDK1 (phospho Y15) antibody (ab47594) at 1/500 dilution + HepG2 cell extract
Predicted band size: 34 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted? -
Western blot - Anti-CDK1 (phospho Y15) antibody (ab47594)This image is courtesy of an anonymous abreview.All lanes : Anti-CDK1 (phospho Y15) antibody (ab47594) at 1/1000 dilution
Lane 1 : untreated HeLa cell lysate
Lane 2 : HeLa cell lysate treated with nocodazole (500ng/mL) for 16 h
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : polyclonal goat HRP conjugated at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 34 kDa
Observed band size: 34 kDa
Exposure time: 1 minuteBlocking: 5% milk for 1 hour at 25°C.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (41)
ab47594 は 41 報の論文で使用されています。
- Yan JX et al. Design and synthesis of guaianolide-germacranolide heterodimers as novel anticancer agents against hepatocellular carcinoma. Drug Dev Res 84:1285-1298 (2023). PubMed: 37345274
- Xu X et al. Post-translational modification of CDK1-STAT3 signaling by fisetin suppresses pancreatic cancer stem cell properties. Cell Biosci 13:176 (2023). PubMed: 37743465
- Debnath S et al. Eriodictyol mediated selective targeting of the TNFR1/FADD/TRADD axis in cancer cells induce apoptosis and inhibit tumor progression and metastasis. Transl Oncol 21:101433 (2022). PubMed: 35462210
- Liu JCY et al. Mechanism and function of DNA replication-independent DNA-protein crosslink repair via the SUMO-RNF4 pathway. EMBO J 40:e107413 (2021). PubMed: 34346517
- Zhang X et al. Treatment of Melanoma by Nano-conjugate-Delivered Wee1 siRNA. Mol Pharm 18:3387-3400 (2021). PubMed: 34375118