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リコンビナントRabMAb

Anti-CDK1 抗体 [YE324] (ab32094)

Reviews (1) Submit a question References (24)
Western blot - Anti-CDK1 antibody [YE324] (ab32094)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDK1 antibody [YE324] (ab32094)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDK1 antibody [YE324] (ab32094)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDK1 antibody [YE324] (ab32094)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDK1 antibody [YE324] (ab32094)
  • Anti-CDK1 antibody [YE324] (ab32094)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [YE324] to CDK1
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 594 Alexa Fluor® 647 Alexa Fluor® 750 APC Carrier Free PE

製品の概要

  • 製品名

    Anti-CDK1 antibody [YE324]
    CDK1 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [YE324] to CDK1

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IHC-Pmore details

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: Hela cell lysate. IHC-P: Human lymphoma tissue.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab32094の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/10000. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa).

For unpurified - use at 1/2000 dilution
IHC-P
1/800.
特記事項
WB
1/10000. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa).

For unpurified - use at 1/2000 dilution
IHC-P
1/800.

ターゲット情報

  • 機能

    Plays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. p34 is a component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II.

  • 組織特異性

    Isoform 2 is found in breast cancer tissues.

  • 配列類似性

    Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
    Contains 1 protein kinase domain.

  • 細胞内局在

    Nucleus.
  • Information by UniProt

  • 参照データベース

    see all

  • 製品の状態

    CDK1 can be located to the Nucleus, cytoplasm and Mithocondria. It's cytoplasmic during interphase and reversibly translocated from cytoplasm to the nucleus when phosphorilated before G2-M transition when associated with cyclin-B1. Accumulates in mitochondria in G2-arrested cells upon DNA-damage.

  • 別名

    • Cdc 2 antibody
    • Cdc2 antibody
    • CDC28A antibody
    • CDK 1 antibody
    • CDK1 antibody
    • CDK1_HUMAN antibody
    • CDKN1 antibody
    • CELL CYCLE CONTROLLER CDC2 antibody
    • Cell division control protein 2 antibody
    • Cell division control protein 2 homolog antibody
    • Cell division cycle 2 G1 to S and G2 to M antibody
    • Cell division protein kinase 1 antibody
    • Cell Divsion Cycle 2 Protein antibody
    • Cyclin Dependent Kinase 1 antibody
    • Cyclin-dependent kinase 1 antibody
    • DKFZp686L20222 antibody
    • MGC111195 antibody
    • p34 Cdk1 antibody
    • p34 protein kinase antibody
    • P34CDC2 antibody
    see all

画像

  • Western blot - Anti-CDK1 antibody [YE324] (ab32094)
    Western blot - Anti-CDK1 antibody [YE324] (ab32094)
    All lanes : Anti-CDK1 antibody [YE324] (ab32094) at 1/10000 dilution (Purified)

    Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
    Lane 2 : RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate
    Lane 3 : PC-12 (Rat adrenal gland pheochromocytoma ) whole cell lysate

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 34 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDK1 antibody [YE324] (ab32094)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDK1 antibody [YE324] (ab32094)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue sections labeling CDK1 with Purified ab32094 at 1:800 dilution (0.90 µg/ml). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control. 

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDK1 antibody [YE324] (ab32094)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDK1 antibody [YE324] (ab32094)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat spleen tissue sections labeling CDK1 with Purified ab32094 at 1:800 dilution (0.90 µg/ml). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control. 

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDK1 antibody [YE324] (ab32094)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDK1 antibody [YE324] (ab32094)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse spleen tissue sections labeling CDK1 with Purified ab32094 at 1:800 dilution (0.90 µg/ml). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control. 

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDK1 antibody [YE324] (ab32094)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDK1 antibody [YE324] (ab32094)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma sections labeling CDK1 with Purified b32094 at 1:800 dilution (0.90 µg/ml). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control. 

  • Anti-CDK1 antibody [YE324] (ab32094)
    Anti-CDK1 antibody [YE324] (ab32094)

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (24)

ab32094 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab32094 は 24 報の論文で使用されています。

  • Du J  et al. MDIG-mediated H3K9me3 demethylation upregulates Myc by activating OTX2 and facilitates liver regeneration. Signal Transduct Target Ther 8:351 (2023). PubMed: 37709738
  • Liu W  et al. Suppression of CCT3 inhibits melanoma cell proliferation by downregulating CDK1 expression. J Cancer 13:1958-1971 (2022). PubMed: 35399722
  • Zhang T  et al. Downregulation of SHCBP1 Inhibits Proliferation, Migration, and Invasion in Human Nasopharyngeal Carcinoma Cells. Evid Based Complement Alternat Med 2021:8262502 (2021). PubMed: 34484405
  • Che J  et al. Neochlorogenic acid enhances the antitumor effects of pingyangmycin via regulating TOP2A. Mol Med Rep 23:N/A (2021). PubMed: 33355372
  • Pal-Ghosh R  et al. CDC2 Is an Important Driver of Vascular Smooth Muscle Cell Proliferation via FOXM1 and PLK1 in Pulmonary Arterial Hypertension. Int J Mol Sci 22:N/A (2021). PubMed: 34203295
View all Publications for this product

レビューと Q&A

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レビューを送る 質問を送る

Immunohistochemistry (Frozen sections) abreview for Anti-CDK1 antibody [YE324]

 Excellent
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Pig Tissue sections (granulosa cells)
Permeabilization
No
Specification
granulosa cells
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Qi Yu

Verified customer

投稿 Feb 07 2024

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