Anti-CDK1 + Cdk2 + Cdk3 (phospho T14) 抗体 [E161] (ab32384)

Blocking/Diluting buffer 5% NFDM/TBST

34kDa band represents endogenous CDK, and 62kDa represents CDK-GFP.

Immunofluorescence staining of HeLa cells with purified ab32384 at a working dilution of 1/200, counter-stained with DAPI. The secondary antibody was Alexa Fluor^® 488 goat anti-rabbit (ab150077), used at a dilution of 1/1000. ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with ab150120 (Alexa Fluor^® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified ab32384 was used at a dilution of 1/500 followed by an Alexa Fluor^® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500. For negative control 2, ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor^® 488 goat anti-rabbit antibody (ab150077) at a dilution of 1/400.

Blocking/Diluting buffer 

Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST

Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST

ab32384 (purified) at 1/50 immunoprecipitating CDK1 in 10 μg HEK293 (Lanes 1 and 2, observed at 34 kDa). Lane 3 - PBS. For western blotting, HRP Veriblot for IP Detection Reagent (ab131366) was used for detection (1/10 000). Blocking buffer and concentration: 5% NFDM/TBST Dilution buffer and concentration: 5% NFDM/TBST

Unpurified ab32384, at a 1/50 dilution, staining Cdc2 in paraffin embedded human lymphoma tissue sections by Immunohistochemistry.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.