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AB1377

Anti-CDCP1 抗体

Anti-CDCP1 antibody

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(19 Publications)

Goat Polyclonal CDCP1 antibody. Suitable for IP, WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 19 publications. Immunogen corresponding to Synthetic Peptide within Human CDCP1 aa 800 to C-terminus.

別名を表示する

CD318, TRASK, UNQ2486/PRO5773, CDCP1, CUB domain-containing protein 1, Membrane glycoprotein gp140, Subtractive immunization M plus HEp3-associated 135 kDa protein, Transmembrane and associated with src kinases, SIMA135

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-CDCP1 antibody (AB1377)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-CDCP1 antibody (AB1377)

ICC/IF image of ab1377 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab1377, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 donkey anti-goat IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Western blot - Anti-CDCP1 antibody (AB1377)
  • WB

Unknown

Western blot - Anti-CDCP1 antibody (AB1377)

ab1377 (1 µg/ml) staining CDCP1 human colon whole cell lysate (35 µg) in RIPA buffer. Incubation was for 1 hour and detection was by chemiluminescence.

All lanes:

Western blot - Anti-CDCP1 antibody (ab1377)

Predicted band size: 92 kDa

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Key facts

宿主種

Goat

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

No

交差種

Human

アプリケーション

ICC/IF, IP, IHC-P, WB

applications

免疫原

Synthetic Peptide within Human CDCP1 aa 800 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

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特異性

This antibody is expected to recognize isoform 1 (NP_073753.3) only. Approx 90kDa band observed in Human Colon lysates (calculated MW of 92.3kDa according to NP_073753.3). Recommended concentration: 1-3µg/ml. An additional band of unknown identity was also consistently observed at 200kDa. This band was successfully blocked by incubation with the immunising peptide.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p>1 hour primary incubation is recommended for this product.</p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "5 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Immunogen
精製に関する特記事項
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
バッファー組成
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: Tris buffered saline, 0.5% BSA
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

May be involved in cell adhesion and cell matrix association. May play a role in the regulation of anchorage versus migration or proliferation versus differentiation via its phosphorylation. May be a novel marker for leukemia diagnosis and for immature hematopoietic stem cell subsets. Belongs to the tetraspanin web involved in tumor progression and metastasis.
See full target information CDCP1
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文献 (19)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 16:1585597 PubMed40391211

2025

Itolizumab regulates activating and inhibitory signals on effector cells, improving their cytotoxicity against CD318+ tumor cell lines.

Applications

Unspecified application

Species

Unspecified reactive species

Cynthia González Muñoz,Rydell Álvarez Arzola,Adanays Calvo Pérez,Milagro de la Caridad Frometa Campanón,Patricia Hernández Casaña,Aymé Fernández-Calienes Valdés,Patricia Lorenzo-Luaces,Zaima Mazorra Herrera,Tania Crombet Ramos,Mayrel Labrada Mon

Journal for immunotherapy of cancer 12: PubMed39455095

2024

Targeting CDCP1 boost CD8+ T cells-mediated cytotoxicity in cervical cancer via the JAK/STAT signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Hua Huang,Yuwen Pan,Qiuwen Mai,Chunyu Zhang,Qiqiao Du,Yuandong Liao,Shuhang Qin,Yili Chen,Jiaming Huang,Jie Li,Tianyu Liu,Qiaojian Zou,Yijia Zhou,Li Yuan,Wei Wang,Yanchun Liang,Chao Yun Pan,Junxiu Liu,Shuzhong Yao

Molecular oncology 17:1648-1665 PubMed37013960

2023

Inhibition of CDCP1 by 8-isopentenylnaringenin synergizes with EGFR inhibitors in lung cancer treatment.

Applications

Unspecified application

Species

Unspecified reactive species

Sze-Ching Wong,Chun-Chieh Yeh,Xun-Yu Zhang,Chih-Ying Hsieh,Chia-Chien Lo,Ting-Ting Kuo,Ching-Chan Lin,Chih-Hua Chao,Jing-Pei Liu,Ling-Chu Chang,Lu-Hai Wang,Yuh-Pyng Sher

Cancer cell international 22:151 PubMed35410293

2022

Molecular mechanism by which CDCP1 promotes proneural-mesenchymal transformation in primary glioblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Zhiying Lin,Zhu Zhang,Haojie Zheng,Haiyan Xu,Yajuan Wang,Chao Chen,Junlu Liu,Guozhong Yi,Zhiyong Li,Xiaoyan Wang,Guanglong Huang

Aging 13:12224-12238 PubMed33882457

2021

Methylation of microRNA-338-5p by EED promotes METTL3-mediated translation of oncogene CDCP1 in gastric cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Fangbin Zhang,Yan Yan,Xinguang Cao,Jinping Zhang,Yingxia Li,Changqing Guo

American journal of cancer research 10:3828-3837 PubMed33294270

2020

Resveratrol-mediated ADAM9 degradation decreases cancer progression and provides synergistic effects in combination with chemotherapy.

Applications

Unspecified application

Species

Unspecified reactive species

Yu-Sen Lin,Chih-Ying Hsieh,Ting-Ting Kuo,Ching-Chan Lin,Chen-Yuan Lin,Yuh-Pyng Sher

Cancer management and research 12:3759-3769 PubMed32547212

2020

CUB Domain-Containing Protein-1 Promotes Proliferation, Migration and Invasion in Cervical Cancer Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Lijun Huang,Yihong Chen,Shuyu Lai,Hongmei Guan,Xiaoling Hu,Jie Liu,Hanrong Zhang,Zhenfei Zhang,Jueyu Zhou

Oncogene 35:4762-72 PubMed26876198

2016

CDCP1 cleavage is necessary for homodimerization-induced migration of triple-negative breast cancer.

Applications

IF

Species

Unspecified reactive species

H J Wright,J Arulmoli,M Motazedi,L J Nelson,F S Heinemann,L A Flanagan,O V Razorenova

Scientific reports 5:16426 PubMed26553452

2015

ADAM9 enhances CDCP1 protein expression by suppressing miR-218 for lung tumor metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Kuo-Liang Chiu,Ting-Ting Kuo,Qian-Yu Kuok,Yu-Sen Lin,Chung-Hung Hua,Chen-Yuan Lin,Pei-Yuan Su,Liang-Chuan Lai,Yuh-Pyng Sher

BMC cancer 14:754 PubMed25301083

2014

CUB domain containing protein 1 (CDCP1) modulates adhesion and motility in colon cancer cells.

Applications

IP

Species

Human

David J Orchard-Webb,Thong Chuan Lee,Graham P Cook,G Eric Blair
View all publications
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